NTU Journal of Pure Sciences

Corona virus2019(Covid-19) or severe Acute respiratory syndrome coronavirus-2 (SARS-Cov-2) has been reported by the World Health Organization (WHO) are global clinical biomarkers have been important in determining its severity, although its aspect have been well characterized, so the aim of this study is to investigate some common alteration in some biochemical parameters in serum of covid-19patients (hospitalized and non-hospitalized, vaccinated and control group.

Study Design: The study conducted across sectional study in cohort of 167(40 hospitalized covid-19 whom paid a visit to Al-Shifaa-14 hospital, Kirkuk, from Iraq, 30 non-hospitalized covid-19, 67vaccinated individuals and 30 control healthy group), from december20,2021 till 20 March 2022. Their ages ranging from (20-75) years. The total 167 patients ,79 males and 88 were females.

Age, comorbidities, and abnormalities in several clinical biomarkers have all been shown to be important in determining the severity of covid-19 illness in several investigations. Although the clinical aspects of COVID-19 have been well characterized, the assessment of changes in the most common biochemical indicators reported in COVID-19 patients is still lacking. So the study aimed to investigate common alterations in some biochemical parameters in the serum of COVID-19 patients (hospitalized and non-hospitalized), vaccinated individuals and control group.

Sampling: Blood samples were collected from 167 individuals, then centrifuged to obtain serum for the biochemical tests that included B. urea and creatinine, Glutamic-pyruvic transaminase(GPT), Glutamic-oxaloacetic transaminase(GOT) and alkaline phosphatase (ALP). Nasopharyngeal swabs were collected from non-hospitalized of COVID-19 infection which was confirmed using real-time PCR technique. The study's findings revealed substantial variations in biochemical parameters between the examined groups in terms of creatinine (value >0.001) and urea (value >0.0001), as well as significant differences in GOT (value >0.0001) and ALP (value >0.0001). So this study can have concluded that patients infected with Covid-19 (hospitalized and infected outside the hospital- subsequently) have an increase in most biochemical parameters, however the effect was lower in the vaccinated group.

Pseudomonas aeruginosa is one of the most prevalent nosocomial pathogenic microorganisms that cause life-threatening infections. The formation of biofilm is one of the important features that lead resistance to antibiotics. The study aimed to clarify the relationship between biofilm formation, antibiotic resistance, and pigment production P. aeruginosa. A total of 33 isolates have been obtained from 220 different clinical samples, 42% of isolates obtained from burn swabs,15% wound swabs,12% from urine samples, 12% ear swabs ,6% sputum samples, and 3% from bronchial washes. The highest prevalence of these bacteria in our study was found in isolates obtained from burn swabs. Pyocyanin and pyoverdine are frequently seen on Cetrimide agar. P. aeruginosa can be completely isolated on Cetrimide agar. The production of biofilms detected by microtiter plate method and the result distributed as, 22% of strongly, 50% moderately, 27% weakly, and 4% were non biofilm producer. The Kirby-Bauer method (disc diffusion susceptibility method) was used to measure antibiotic resistance, and the results revealed a correlation as 86% multidrug resistance were strongly biofilm former, 75% multidrug resistance were moderately biofilm former and 11% multidrug resistance were weakly biofilm producers.

Conclusion, P. aeruginosa from clinical isolates has highly proportional capability to form biofilm and recorded a positive correlation with multidrug resistance, while this relation was not significant with pigment production.

The aim of the research is to identify the main factors affecting the evaluation of the annual performance of the teaching staff using the Binary Logistic Regression technique by applying to a random sample of size(217) teaching and teaching staff from four different disciplines (technology, administrative, agricultural, The results of the analysis showed that the completion and publication of scientific research was the most important factor affecting the evaluation of the performance of the teaching staff, followed by the teaching use of modern teaching methods and its incorporation of technology into education.

The estimation of the parameters is one of an important issues in the mathematical statistics. The development of estimation methods requires accurate estimation and finds the best estimator for parameters. The aim of this research is to build a regression model. A dependent variable has a truncated t-distribution for this model. It was depended to the method of maximum likelihood for finding the parameters of the model, the parameters ? and ? 2 were estimated when they were unknown, the approximation of the cumulative function of the truncated t-distribution from two sides is used to represent the function of the dependent variable. It was concluded that the value ? is equal ?ols plus a certain amount, this amount is equal zero when the values of the truncated points a, b are equal in value and different in sign.

As a practical application, simulation was used in data generation by using the program (Matlab 2020a), the value of ? was estimated, The comparison was between maximum likelihood method and according to the formula that we reached with its estimated value according to the least squares method based on the mean square errors, where its value according to the maximum likelihood method was less than its value according to the method of least squares, which indicates the advantage of the first method over the second.

Severe acute respiratory syndrome coronavirus 2 (SAR-CoV-2) is the novel infectious disease agent that causes COVID-19. The laboratory confirmation of COVID-19 is based on nucleic acid-based virus genome sequencing and real-time PCR and serological methods, rapid antigenic testing. The aim of the study was to evaluate the diagnostic sensitivity of the immunochromatography method by comparing it with PCR in covid-19 diagnosis. The study involved 116 samples, 90 samples included covid-19 patients and 26 samples included healthy, as a control group. Two nasopharyngeal swabs (NP) were taken, one for an antigenic test cassette and the other swab for re-confirmation of the infection by a real-time RT-PCR. The result of this study showed that there was a significant difference (P value = 0.0001) between RT PCR and rapid Ag tests in patients and healthy groups. The low sensitivity recorded for rapid antigen detection kits was 69% and 70%, respectively, compared with the 100% sensitivity of PCR. This study demonstrated an elevated positive rate of rapid Ag positive during the period (8–14 days) of symptoms onset. This study concluded that the rapid antigen test is a helpful tool for detecting the presence of COVID-19 infection, but it may not be the best alternative due to its low sensitivity.

The most common non-viral sexual transmission disease in humans, trichomoniasis, is caused by the protozoan parasite Trichomonas vaginalis (STD). Metronidazole is the most frequently recommended of the imidazole drugs used to treat trichomoniasis. In any case, these substances may result in undesired side effects and parasite resistance. As a result, this infection needs to be treated in a different way.

Aim: This study was designed to determine the relation between zinc intake and the Trichomonas vaginalis occurrence and also to find out the in vitro effect of zinc on T. vaginalis viability.

Methods: This study was carried out on 63 pregnant women whom attended (Azadi Teaching, Kirkuk General and Al-Nasr maternity) Hospitals from December 2021 till April 2022. Specimens of vaginal swabs and serum samples were obtained from all pregnant women. The swabs were first examined directly by normal saline on slide microscopically then inoculated onto Modified Diamond medium. Zinc levels and concentrations in the blood were assessed using serum. Result: T. vaginails was isolated from pregnant women. The overall prevalence among 63 examined samples were positive by direct microscopically and culture with a rate of 5(8%) while 58 (92%) were negative. The 1.5mg/ml zinc concentration was the most effective on T. vaginalis trophozoites number than other concentration of zinc. Conclusion: Zinc is a very important anti- T. vaginalis and can be used it as dilution washing for treatment process.