Abstract
ABSTRACT
The research aimed to investigate the effect of salicylic acid, and some plant extracts some factors on growth indicators and stimulating cardiac glycosides in digitalis leaves, in the Plant Tissue Culture Laboratory - College of Agricultural Engineering Sciences - University of Baghdad from 10-2-2022 to 6-1-2023. Branch cultures of the digitalis plant were obtained from planting the 1 cm length shoot tips, grown from sterilized seeds cultured in MS medium provided with 0.5 mg.L-1 of BA. After obtaining a sufficient number of vegetative branches by proliferating them more than once, they were cultured in MS medium provided with salicylic acid at the concentrations of 0, 1, 3, 5, and 7 mg.L-1, ginger extract at the concentrations of 0, 3, 5 and 7 g.L-1, and black pepper extract at the concentrations of 0, 3,5, and 7 g.L-1 as independent experiments. The results showed that salicylic acid concentration was superior 3mg.L-1 was significantly superior in the number of branches, number of leaves, fresh and dry weight, chlorophyll content, and the cardiac digoxin, reordering 2.67 branches.plant-1, 0.467cm, 9.67 leaves.plant-1, 1.328 g, 0.879 g., 1.376 mg, and 28.48 µg.g-1; and the treatment of ginger extract at a concentration of 5 gm l-1 and black pepper extract at a concentration of 3 gm l-1 was significantly superior separately in same traits, attaining 4.33 branches.plant-1, 14.43 leaves.plant-1, 1.567 g, 0.823 g, 1.497mg, and 29.32 µg.g-1 for ginger extract; and 2.133 branches.plant-1, 1,243 cm, 14.99 leaves.plant-1, 1.174 g, 0.659 g, 0.855 g.100 g-1fresh weight, and 29.66 µg.g-1 for black pepper extract, respectively.
The research aimed to investigate the effect of salicylic acid, and some plant extracts some factors on growth indicators and stimulating cardiac glycosides in digitalis leaves, in the Plant Tissue Culture Laboratory - College of Agricultural Engineering Sciences - University of Baghdad from 10-2-2022 to 6-1-2023. Branch cultures of the digitalis plant were obtained from planting the 1 cm length shoot tips, grown from sterilized seeds cultured in MS medium provided with 0.5 mg.L-1 of BA. After obtaining a sufficient number of vegetative branches by proliferating them more than once, they were cultured in MS medium provided with salicylic acid at the concentrations of 0, 1, 3, 5, and 7 mg.L-1, ginger extract at the concentrations of 0, 3, 5 and 7 g.L-1, and black pepper extract at the concentrations of 0, 3,5, and 7 g.L-1 as independent experiments. The results showed that salicylic acid concentration was superior 3mg.L-1 was significantly superior in the number of branches, number of leaves, fresh and dry weight, chlorophyll content, and the cardiac digoxin, reordering 2.67 branches.plant-1, 0.467cm, 9.67 leaves.plant-1, 1.328 g, 0.879 g., 1.376 mg, and 28.48 µg.g-1; and the treatment of ginger extract at a concentration of 5 gm l-1 and black pepper extract at a concentration of 3 gm l-1 was significantly superior separately in same traits, attaining 4.33 branches.plant-1, 14.43 leaves.plant-1, 1.567 g, 0.823 g, 1.497mg, and 29.32 µg.g-1 for ginger extract; and 2.133 branches.plant-1, 1,243 cm, 14.99 leaves.plant-1, 1.174 g, 0.659 g, 0.855 g.100 g-1fresh weight, and 29.66 µg.g-1 for black pepper extract, respectively.
Keywords
black pepper extract.
Digoxin
ginger extract
Micro propagation
Abstract
نفذت التجربة بهدف اختبار تاثير حامض السالسيليك وبعض المستخلصات النباتية في المؤشرات الخضرية وتحفيز اهم مركبات الكلايكوسيدات القلبية لاوراق نبات الدجتالس، في مختبر زراعة الانسجة النباتية- كلية علوم الهندسة الزراعية – جامعة بغداد للمدة من 2-10 - 2022 ولغاية 1-6-2023 .تم الحصول على مزارع الافرع الخضرية لنبات الدجتالس من زراعة اطراف الافرع بطول 1سم والتي تم الحصول عليها من البذور المعقمة والمزروعة في وسط MS المجهز بالـBA بالتركيز ( 0.5 ملغم .لتر 1-) ولحين الحصول على العدد الكافي من الافرع الخضرية زرعت في الوسط MS المجهز بحامض السالسيليك بالتراكيز (0 ، 1 ، 3 ، 5 ،7 ملغم .لتر -1) و الوسط MS المجهز بمستخلص الزنجبيل بالتراكيز (0 ، 3 ، 5 ،7 غم .لتر 1-) و الوسط MS المجهز بمستخلص الفلفل الاسود بالتراكيز (0 ، 3 ، 5 ،7 غم .لتر -1) في تجارب مستقلة ، اظهرت نتائج تجربة حامض السالسيليك تفوق التركيز ( 3 ملغم .لتر -1) معنويا في عدد الافرع وعدد الاوراق والوزن الرطب والجاف والكلوروفيل وdigoxin القلبية ( 2.67 فرع . نبات 1- ، 9.67 ورقة . نبات 1- ، 1.328 غم ، 0.879غم 1.376 ملغم 28.48 مايكروغرام. غم-1 ، كما تفوقت معاملة مستخلص الزنجبيل بتركيز5غم .لتر1- ومستخلص الفلفل الاسود بتركيز 3غم . لتر 1- كلا على حدي معنوياً في معدل الصفات نفسها اذ بلغت 4.33فرع .نبات-1 ، 14.43 ورقة . نبات-1 ، 1.567غم ، 0.823غم ، 1.497 ملغم .وزن طري ، 29.32 مايكروغرام.غم-1 ،2.133 فرع. نبات 1- ، 1.243 سم ، 14.99 ورقة. نبات 1- ، 1.174 غم ،0.659 غم ،0.855غم100. غم وزن طري 29.66 مايكروغرام. غم 1- ).
Keywords
الاكثار الدقيق ، مستخلص الزنجبيل، digoxin ، مستخلص الفلفل الاسود