Al-Kufa University Journal for Biology

115 samples were collected (42 wound samples, 38 burn samples, 17 urine samples, 10 ear infections and 8 vaginal samples) from Hilla Teaching Hospital and Babylon Maternity and Children's Hospital in Babylon Governorate for the period between September 2010-June 2011. 45 isolates of Pseudomonas aeuroginosa bacteria were obtained and the isolates were identified according to morphological and biochemical tests and using the Api 20 E system. The percentage of isolation from different infection sites was as follows: wound infections 18 isolates (40%) and burns 15 isolates ((33..33%), and the P. aeruginosa isolates isolated from urine came in third place 8 (17.77%). Followed by those isolated from the ear 3 (6.66%) and then the isolates isolated from the vagina 1 (2.22%).
The polysaccharide was extracted The lipid of Pseudomonas aeuroginosa bacteria isolated from wounds. New Zealand white rabbits (using three animals for each experiment) were immunized at the age of 2-3 months with the lipopolysaccharide antigen as well as using the complete Freunds immune adjuvant. After the end of the immunization period, some criteria of the systemic humoral immune response of rabbits immunized with the bacterial antigen were determined and compared with the control animal immunized with normal saline. The results of the current study indicated that the germ antigen led to a humoral systemic immune response that was determined using the diffusion immunoassay to determine the concentration levels of immunoglobulins (IgA, IgM, and (IgG) and complement components (C3 and C4) and the total protein concentration rate, as the concentration rate of immunoglobulins, complement components, and protein concentration rate for the test animals increased, as the concentration rate of IgM reached (304.3) mg/dl and the concentration rate of IgG was (1907.4.) mg/dl, while the IgA rate reached (493.6) compared to the control animals, as the levels of IgM, IgG, and IgA reached 208.7, 1488.4, and 433.6, respectively. As for the third complement component, it reached (93.4) ​​mg/dl and the concentration rate of the fourth component reached (13.4) mg/dl, and the total protein concentration rate also increased, as it reached (15.3) gm/dl. All these values ​​are for animals immunized with lipopolysaccharide antigen and the results of all tests were significant at the probability level (P<0.05) when compared with control animals.

This study was conducted at the Fertility Center / Al-Sadr Medical City / Al-Najaf Governorate for the period from 11/1/2012 to 4/30/2013. The semen samples were divided into two groups: fertile men, whose number was 17 samples (Fertile Normozoospermia), and patients with asthenozoospermia, whose number was (32) samples. The average age of fertile men was (1.36±30.9) and for patients with asthenozoospermia (1.63±31.4). The total number of samples was (49) samples. This study aimed to know the relationship between the concentration of Malondialdehyde (MDA) and sperm parameters and the percentage of abnormal sperm chromatin in patients with asthenozoospermia. The results of this study showed a significant decrease (p<0.05) in the concentration of sperm, the percentage of sperm with progressive motility and the percentage of normal sperm in patients with asthenozoospermia compared to fertile men, while a significant increase (p<0.05) was observed in the concentration of Malondialdehyde (MDA) and the percentage of abnormal sperm chromatin in patients with asthenozoospermia compared to fertile men. The results of this study also showed a negative relationship between the concentration of Malondialdehyde (MDA) and sperm parameters represented by the concentration of sperm (r=-0.5543, p<0.05), and the percentage of sperm with progressive motility (R=-0.7756,P<0.05), and the percentage of normal sperm, while a positive relationship was observed between the concentration of malondialdehyde (MDA) and the percentage of abnormal sperm chromatin (r=0.7066,P<0.05). It is concluded from the current research that an increase in the concentration of malondialdehyde (MDA) negatively affects sperm parameters and the integrity of sperm chromatin.

During the period from November 15, 2009 to July 10, 2010, 256 samples were examined, including vaginal swabs (soft drops), urine samples from women suffering from reproductive and urinary system problems in Babylon Governorate, in addition to 4 samples from some couples in an attempt to determine the prevalence of the Trichomonas vaginalis parasite among women visiting the Maternity Hospital in Hillah city and some health centers in addition to some private clinics, which examined these samples by wet mount and centrifugation of urine samples. The results were as follows:
# The first group: included married and unmarried women. The infection rate with the parasite in general was 21.87%, while the infection rate among married women was 21.68%, in addition to diagnosing the parasite among unmarried women, with the parasite being observed in some couples. #The second group: included rural and urban women, and the infection rate of married women in the countryside was higher than in the city, reaching 24.85% and 14.47% respectively, with significant differences.

#The third group: showed the infection rate of housewives and employees, and it was higher among employees at 31.25% compared to housewives at 21.03% without reaching significant differences.

#The fourth group: included age groups, as the highest infection rate was 31.50% among the age group 26-30 compared to other groups, with significant differences.

The present study aims to determine the incidence of giardia parasite in different ages and genders in Diwaniyah city and Afak district for the period from 3/1/2011 to 3/6/2011 in addition to showing the effect of parasitic infection on the level of hemoglobin in the blood. The study included examining (760) random samples of stool and blood from people visiting Diwaniyah General Hospital in Diwaniyah city center and the health center in Afak district (semi-rural area) and from both genders. The results of the examination recorded (102) positive cases (ve+) and (658) negative cases (ve-). The results of the study showed the highest rate of infection with the parasite in Afak district (69.5%), while the rate was (30.5%) in the center of Diwaniyah city. As for age, the infection rate in children was (70.5%) compared to adults (29.5%). On the other hand, the infection rate in females (79.5%) was higher than males (20.5%). The study showed that most of the infected children suffer from low hemoglobin and a rate of (70%), while adults were (30%). The study recommendations include health awareness and education for all members of society and attention to personal hygiene. Drinking sterile water and using modern methods of sanitary drainage of sewage.

The study was conducted in the laboratory of the Department of Life Sciences at the University of Babylon during the period from 15-1-2014 to 15-4-2014. The study included the effect of two factors, the first is auxin dissolved in distilled water and the second is auxin dissolved in distilled water treated magnetically with three magnetic flux intensities of (1000, 2000, 3000 Gauss) and studying their effect on some physiological and biochemical characteristics (rooting characteristic, CAT, SOD, MDA content, GSH content) in the cuttings of the mung bean plant. The study showed that auxin dissolved in distilled water treated magnetically with a magnetic intensity of 1000 Gauss showed significant differences in most of the studied characteristics compared to distilled water.