Al-Kufa University Journal for Biology

The current the study is carried out investigate of antimicrobial activity of uracil isophenol compound against some bacteria pathogens causing UTI infection . The urine sample were collecting to hospitals in Najaf of (AL Sadr Medical City ) for the period from (2 in July 2013 to 2 in September 2013 up) The results were as follows: The proportion of isolated bacteria is E.coli 40% higher and the proportion of bacteria isolated is Staphylococcus aureus 32% and isolation of bacteria Pseudomonas.aeruginosa 28% in addition to some of the genera were diagnosed. .
In the course of the program directed towards the synthesis of fused nitrogen heterocyclic compound and as an extension of efforts directed towards the development of convenient synthetic approaches for the synthesis one of pyrimidine derivatives called uracil isophenol with an expected broad spectrum of biological activity. Studies have shown that uracil isophenol exhibit various biological activities. In biological study was to demonstrate in trial the new compound "uracil isophenol" in different concentration observed the following E.coli exhibit sensitivity to all concentration with differ inhibition zone size ; but the same zone size for (10,15,20) mg/ml the inhibition area was reached for 21 mm . in spite of difference in concentration. While Staphylococcus aureus also sensitive and the zone larger than E.coli ,and have the same zone size of E.coli of concentration(3)mg/ml was reached for 15 mm . While Pseudomonas.aeruginosa also exhibit sensitivity but less than others except for (25) mg/ml it have inhibition zone was reached for size of 25 mm . Results were compared with control groups were analyzed statistically by results analysis system completely random ANOVA and LSD.

The macrophyte distribution of Majnoon Marsh is described in relation to water depth, light penetration, turbidity, air temperature, water temperature, pH, electrical conductivity, salinity, dissolved oxygen, chloride, reactive phosphate, reactive nitrate, and reactive nitrite. Changes in species composition of macrophytes are visualized by means of multivariate analysis. CANOCO ordination programs (CCA) were used to analyze the data set. The classification of 100 stands revealed nine aquatic macrophyte communities, which have 15 aquatic macrophyte species can be recognized in the marsh. These macrophytes species belong to three categories: Submerged species, representing by Ceratophyllum demersum, Myriophyllum spicatum, Najas armata, Najas minor, Potamogeton crispus, Potamogeton lucens, Potamogeton perfoliatus, Potamogeton pectinatus, Hydrilla verticillata, Chara sp.,; free-floating hydrophytes, Lemna minor, Salvinia natans; and the dominant emergent species, Phragmites australis, Typha domingensis, and Schoenoplectus litoralis. Species diversity increases with decreasing salinity and water depth; also, increasing water nutrient and light penetration play important role in increasing species diversity. Free-floating macrophytes (Lemna minor and Salvinia natans) have significant relationship with water nutrient.

Thirty five patients aged between 7 months-17 years were all positive for Leishmania tropica parasite, using Bios Company test kit called ''Kalazar Detect Rapid Test" . Blood cell counts for leukocytes were 4 x103, lymphocytes where, neutrophils were and platelets. the cell count were done using Sysmex model KX-21 cell count, Japan (Sysmex Corporation). Using 12 micro liters blood taken from the patient and using glass slide inserted in pocket in the system. Five patients were spleenactomyzed

Not previously studied metalloprotease was tested in four species of leguminosae, purified and characterized from Acacia farnesiana which have maximum specific activity 25.01 U/mg. Precipitation by 50% ammonium sulfate referred to increase of specific activity (35.84) U/mg while loading on DEAE-Cellulose exchanger pointed out elevation of specific activity and purification fold, 51.39 unit/mg and 2.06 respectively. Molecular mass, pH and temperature optima of purified metalloprotease were 55.11kDa, 7.5 and 50oC respectively. All metal ions were decreased enzyme activity except the zinc showed the increasing about 16%. EDTA was inhibiting the activity in otherwise other inhibitor not affected on enzyme activity. Optimum substrate for activity was BSA and has Km and Vmax 1.11mM and 625 mM/ min respectively.

Objectives: A study was carried out to assess isolation and detection of Moraxella catarrhalis(M. catarrhalis) isolates from 100 specimens received from Children at 1-3 years of age suffered with Acute Otitis Media (AOM) in Al-Kadhemiya Pediatric Hospital.
Methods: The identity of isolates was confirmed by staining method (Gram stain) , culturing, biochemical test(catalase, citrate utilization, indole production, urease production, motility, methyl red, Voges-Proskauer and DNase production) and Sensitivity test.
Results: from 100 samples, 8 isolates of M. catarrhalis were collected. The biochemical activities especially the DNase production, motility, methyl red and catalase were fixed as features of it.
All the isolates were resistant to Ampicillin (AMP), Penicillin (PEN) and Amoxicillin (AMX) because it has the ability to produce ß-lactamase. While the isolates were susceptible to Erythromycin (ERY),Tetracycline (TET) and Chloramphenicol (CLH).
Conclusion: This study showed that M. catarrhalis is one of the main bacterial agents that causing Acute Otitis Media (AOM). The production of DNase enzyme was the characteristic feature of it from others bacterial causing agents of AOM. Also most strains of M. Catarrhalis produce ß-lactamase, which make it resistant to many antibiotics like Ampicillin, Penicillin and Amoxicillin.