Abstract
Periodontal disease, also known as gum disease, is a chronic inflammatory
condition that affects the supporting structures of the teeth, including the gums and
underlying bone. It is primarily caused by a bacterial infection resulting from poor
oral hygiene practices. The main objectives of the current study were to synthesize
iron oxide nanoparticles (Fe2O3-NPs) and their characteristics were analyzed through
UV, SEM, and X-ray analysis. Additionally, the dispersion of the synthesized NPs
was investigated using UV and TEM techniques. The study also aimed to assess the
kinetic behavior and the impact of Fe2O3-NPs on catalase activity in the saliva of the
patient and control groups. The findings revealed a significant increase in catalase
activity (p>0.05) in both serum and saliva of all patient groups compared to the
control group. The UV-visible spectrum shows that the absorption peaks for
synthesized α- and γ-iron oxide nanoparticles was at 225 nm. According to the SEM
image analysis, the primary particle size of the nano-particles is approximately 26.8
nm. The X-ray diffraction results indicate that the Fe2O3-NPs range in size from 14
nm to 26 nm. UV and TEM study conclude that water a suitable solvent for Fe2O3-
NPs dispersion on kinetic study of catalase (CAT) activity. The kinetic data cleared
that the α-Fe2O3-NPs have an inhibitory effect for the total activity of salivary CAT
in the samples of both healthy and patients with chronic periodontitis. Lineweaver–
Burk graph showed that (α-Fe2O3) inhibit catalase by un comp. inhibition. γ-Fe2O3-
NPs inhibited total salivary CAT activity in healthy and patients with chronic
periodontitis samples. Lineweaver –Burk graph showed that (γ-Fe2O3) inhibit catalase
by uncompetitive inhibition.
condition that affects the supporting structures of the teeth, including the gums and
underlying bone. It is primarily caused by a bacterial infection resulting from poor
oral hygiene practices. The main objectives of the current study were to synthesize
iron oxide nanoparticles (Fe2O3-NPs) and their characteristics were analyzed through
UV, SEM, and X-ray analysis. Additionally, the dispersion of the synthesized NPs
was investigated using UV and TEM techniques. The study also aimed to assess the
kinetic behavior and the impact of Fe2O3-NPs on catalase activity in the saliva of the
patient and control groups. The findings revealed a significant increase in catalase
activity (p>0.05) in both serum and saliva of all patient groups compared to the
control group. The UV-visible spectrum shows that the absorption peaks for
synthesized α- and γ-iron oxide nanoparticles was at 225 nm. According to the SEM
image analysis, the primary particle size of the nano-particles is approximately 26.8
nm. The X-ray diffraction results indicate that the Fe2O3-NPs range in size from 14
nm to 26 nm. UV and TEM study conclude that water a suitable solvent for Fe2O3-
NPs dispersion on kinetic study of catalase (CAT) activity. The kinetic data cleared
that the α-Fe2O3-NPs have an inhibitory effect for the total activity of salivary CAT
in the samples of both healthy and patients with chronic periodontitis. Lineweaver–
Burk graph showed that (α-Fe2O3) inhibit catalase by un comp. inhibition. γ-Fe2O3-
NPs inhibited total salivary CAT activity in healthy and patients with chronic
periodontitis samples. Lineweaver –Burk graph showed that (γ-Fe2O3) inhibit catalase
by uncompetitive inhibition.
Keywords
Catalase
Chronic periodontitis
enzyme inhibition
Iron oxide nanoparticles
saliva.