Abstract
Genistein (GEN) is one of the predominant dietary isoflavones found in legumes
such as soybeans. Genistein has been recommended as an osteoporosis treatment for
postmenopausal women and elderly men, with the intention of reducing
cardiovascular disease and hormone-dependent malignancies. Therefore, two
sensitive and simple methods for quantifying it in the supplements preparation were
developed.The first method (A) comprised employing the surfactant Triton X-114 to
extract the result of the diazotization reaction with 4-Aminoacetophenone(4AMA)
utilizing a cloud point extraction technique. The product was extracted using
micelles of a non-ionic surfactant (TritonX-114) and then spectrophotometrically
detected at a specified wavelength of 437nm. The linearity of the calibration curve
was in the range of 0.25 -2.75 μg/mL of GEN(r = 0.9978) under optimal conditions.
The average sample recovery was determined to be between 96 and 101 %. For 2
μg/mL of GEN, the relative standard deviation (RSD) was (0.27%). The second
method (B) involved using the spectrophotometric-flow injection methodology for
the same diazotization coupling reaction described above, and the orange colored
dyewas measured at a wave length of 437 nm.Calibration curve were linear at
different concentration range of GEN 10 to 100 μg/mL , relative standard deviation
RSD% were 0.23 to 1.21. The proposed methods were applied to determine the
presence of GEN in Supplements.
such as soybeans. Genistein has been recommended as an osteoporosis treatment for
postmenopausal women and elderly men, with the intention of reducing
cardiovascular disease and hormone-dependent malignancies. Therefore, two
sensitive and simple methods for quantifying it in the supplements preparation were
developed.The first method (A) comprised employing the surfactant Triton X-114 to
extract the result of the diazotization reaction with 4-Aminoacetophenone(4AMA)
utilizing a cloud point extraction technique. The product was extracted using
micelles of a non-ionic surfactant (TritonX-114) and then spectrophotometrically
detected at a specified wavelength of 437nm. The linearity of the calibration curve
was in the range of 0.25 -2.75 μg/mL of GEN(r = 0.9978) under optimal conditions.
The average sample recovery was determined to be between 96 and 101 %. For 2
μg/mL of GEN, the relative standard deviation (RSD) was (0.27%). The second
method (B) involved using the spectrophotometric-flow injection methodology for
the same diazotization coupling reaction described above, and the orange colored
dyewas measured at a wave length of 437 nm.Calibration curve were linear at
different concentration range of GEN 10 to 100 μg/mL , relative standard deviation
RSD% were 0.23 to 1.21. The proposed methods were applied to determine the
presence of GEN in Supplements.
Keywords
4-Aminoacetophenone
Cloud point extraction
Flow injection analysis
Genistein
Spectrophotometry
Triton X-114