Iraqi Journal of Agricultural Sciences

This study was aimed to test four types of different plants for the purpose of selecting the optimal plant as a source of the amylase enzyme, including wheat, barley, rice, and potatoes. Among these plants, wheat was selected as the best plant source for enzyme extraction because it possessed the highest effectiveness of the enzyme specific activity (3.0 U/mg protein). Furthermore, sodium acetate buffer (0.2 M, pH 6.0) was determined to be the optimal extraction buffer with a 1:5 (w:v) ratio after 75 min, and it provided 68 U/mg protein. The enzyme was concentrated with sucrose before being purified by gel filtration chromatography using Sephadex G-150. The results demonstrated a 1.8-fold increases in final purification folds with a 332% yield of enzymes. At pH 6.0, the purified enzyme showed its highest activity and stability. The activity of the purified enzyme was most effective at 45 °C and remained stable up to 37 °C. At concentrations of 5 and 10 M, various ionic and chemical substances had an impact on amylase.

Indole molecules as a signal has an important role in bacterial ecosystem, this study was aimed and conducted on the extraction and partial purification of indole from pathogenic E. coli and study several indole characterizations parameters compared with synthetic standard one. A total of 134 urine specimens and other stool 152 specimens were obtained from Al-Ramadi Hospitals, during the period from November / 2018 to March / 2019.The results show the percentage of isolated E. coli from Urinary tract infection was 100 (74.6%)/134, While 152 (100%)/ 152 are isolated from stool. Primary and secondary screening concluded, there are ten isolates are considered as the best in production of indole. The most producible one is selected with indole concentration 165.667 µM/ml. Also, indole production needs several optimal parameters to elevate its production, the study improved that the best pH is 9, temperature is 35 °C, incubation period is 18 hrs. and indole concentration is directly proportional with the amount of tryptophan added. Thin layer chromatography result reveals that no significant difference between extracted indole Rf 0.9 and 0.91 of synthetic standard one. Fourier-Transform-Infrared Spectroscopy, the results show there are some differences in the analysis in some structural positions.

Indole mechanism as an interspecies signal particle to adjust various physiological actions, like virulence, antimicrobial and acid resistance. This study was aimed to use partial purified indole extracted from uropathogenic E. coli and study the cytotoxic effect on some cancer cell lines (A375, HepG2, PC3) and comparable with normal cell line (WRL- 68). Serial dilutions of natural and synthetic standard indole were tested (400, 200, 100, 50, 25, 12.5, and 6.25 μg/mL). The results show a low cytotoxic effect for both natural and synthetic indoles in HepG2 and A375 cell lines as well as this degree of cytotoxicity was obviously appeared in normal cell line WRL-68 with no significant differences among them. While, both indoles effect was appeared high cytotoxic effect on PC3 cell line, this increasing has a significant difference with WRL-68. Thus, it can be suggesting that, this type of indole is not favorable for treatment of some types of cancer, and may has a specific biological activity against exact cancer cells.

This study was aimed to produce zinc oxide nanoparticles (ZnO-NPs) using the supernatant of Weissella confusa UPM22MT04 and assess their effectiveness in inhibiting methicillin-resistant Staphylococcus aureus (MRSA). An isolate of Weissella confusa UPM22MT04 was isolated from a wastewater treatment plant in Johor, Malaysia, and was utilized to synthesize ZnO-NPs. The synthesized ZnO-NPs were characterized through several techniques, including UV-visible spectroscopy, Fourier-transform infrared spectroscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, and dynamic light scattering. Monodisperse spherical ZnO-NPs of 1.7 - 7.9 nm were obtained with 0.1 M zinc nitrate at 80°C. The biosynthesized ZnO-NPs exhibited vigorous inhibitory activity against MRSA. Results found that ZnO-NPs inhibited MRSA at a minimum concentration of 0.625 mg/mL and were bactericidal at a minimum concentration of 1.25 mg/mL. In MTT assays, ZnO-NPs showed no toxicity to HS-27 fibroblasts. The supernatant of Weissella confusa UPM22MT04 could be used to synthesize ZnO-NPs, which are an antibacterial agent, eco-friendly and non-toxic.

This experiment was conducted to investigate the effect of different zinc oxide nanoparticle (ZnONPs) concentrations and salicylic acid (SA) on growth characteristics and antioxidant defence from Cucurbita pepo L. callus culture in response to drought stress conditions. The study included two levels of drought induced by sorbitol (0 and 20 g l-1), two concentration of SA (0 and 20 mg l-1), and four concentrations of ZnONPs (0, 100, 200, and 300 ppm). Drought stress by sorbitol showed negative effects on some characteristics of callus culture fresh weight (FW), dry weight (DW), and hydrogen peroxide (H2O2). The exogenous SA showed a positive in most characteristics except for DW, H2O2 and CAT. ZnONPs positively affected all study indicators on tissue culture under drought conditions. The exogenous of both SA and ZnONPs without sorbitol increased significantly in FW and DW. The SA and ZnO NPs with sorbitol increased significantly in biochemical characteristics such as H2O2, SOD, CAT, proline, and phenolic compounds such as coumaric acid, ferulic acid, caffeic acid, luteolin, and rutin.

An experiment was conducted to evaluate different parts of the plant Capparis spinosa L. extracts for their phytochemical compounds by two different solvents (ethanol &hexane) . The current results showed the superiority of the ethanolic extracts by their quantity and quality of active compounds compared to the hexane extracts. The yield percentage of ethanolic Capparis spinosa L extracts ranges between (3-31%), and color range between Dark brown, and olive with oily texture. Whereas, the yield of the hexane extract ranges between (1-27.9%) and it takes a longer time for extract with difficult to obtain, Ultimately, the results of the phytochemical analysis demonstrated the presence of numerous active compounds with higher degree of the sediment color in ethanol extracts such as: alkaloids, Phenols, Glycosides, Tannins, flavonoids and Saponin comparing with hexane extracts with lower color strength.

Several studies have shown that the presence of Helicobacter pylori (H. pylori) may be a risk factor for Ischemic Heart Diseases (IHD). Therefore, it is needed to look into the relation between IHD and H. pylori infection. One hundred Iraqi patients with IHD and 60 healthy Iraqis as a control group participated in the current study. The ELISA assay was used for the measurement of the sera levels for anti- H. pylori IgG, IgM, and Interleukin-6 (IL-6). Also, H. pylori DNA in the human venous blood was investigated by the polymerase chain reaction (PCR) using 16S rDNA primers. Between IHD patients and the control group, H. pylori-IgG and IL-6 mean ± SD were (Patient =1.814±0.08, Control = 0.823±0.09, Patient = 49.73±6.60, Control = 0.21±0.02, respectively), and this results showed a highly significant variation (p < 0.05) in anti-H. pylori IgG and IL-6, while no significant variations (p > 0.05) were found in anti- H. pylori IgM. It was also found that PCR amplification of Helicobacter pylori DNA using 16S rDNA primers was positive in the venous blood of 81 (81%) IHD patients. The current study results proposed that there was a relation between H. pylori and Ischemic Heart Disease.

Beans (Vicia faba) contain some anti-nutritional compounds, especially vicine and convicine and their non-sugar derivatives, divicine and isouramil, which are responsible for the occurrence of Favism in people with hereditary deficiency of Glucose-6-phosphate dehydrogenase (G6PD). These study objective was estimation of vicine and convicine content and their distribution in dry and green bean parts during the growth stages, and the effect of beta-glucosidase from defatted apricot seed powder and some home-based processing conditions on elimination of these compounds from the bean. The results showed that the content of vicine & convicine in bean ranged from 22.7 mg/100 g in the seedling stage to 46.8 mg/100 g in the pods stage at 139 days. As for vicine, it ranged from 12.6 mg /100g to 29 mg/100g at the above stages respectively. While for convicine it ranged from 10.1 mg/100g to 17.8 mg/100 respectively. The enzymatic treatments showed significant elimination of those compounds as compared to non-enzymatic treatments. Additionally, other factors such as soaking temperature, pH, and time as well as cooking process had a positive effect on the elimination of those glucosides.

The objective of this study, is characterization of partially purified beta-glucosidase (BGS) extracted from apricot defatted seeds powder. The enzyme was extracted using sodium phosphate solution (0.2M, pH 7) containing 2% sodium chloride with mixing ratio of (1:5 solid: liquid) for extraction period of 3 hours at 4C. The crude extract was precipitated by ammonium sulfate at 20-70% saturation, dialyzed then concentrated. The specific activity of the enzyme, folds of purification and the enzymatic yield were 5.45 units/mg, 7.8 fold and 84.5%, respectively. The results showed that the optimum conditions for the enzyme activity were at pH 5.5 and 6.5, the optimum temperature for the enzyme’s activity was 40°C and 55°C, while the thermal stability of the enzyme was between 25-45°C when the enzyme retained more than 90% of its activity over 30 minutes incubation at the optimum pH for activity, and retained 88% of its activity at 50° C. The results also revealed the inhibitory and activator effects of minerals and chemical compounds on the BGS activity.

This study was aimed to investigate active compounds in the aqueous extract of Ganoderma applanatum mycelium. The aqueous extract of fungus mycelium was prepared and some active compounds were detected, including phenols, flavonoids, tannins, alkaloids, glycosides and resins, all of which gave a positive detection. The effective compounds were diagnosed by GC-Mass Chromatography technique, was revealed that there are a number of compounds that can produce mycelium with ability to inhibit microorganisms, inhibit free radicals and prevent oxidation, as well as their effect in reducing cancerous tumors. As for the effective groups, they were diagnosed by FTIR technique.

This research was carried out to investigate the effect of chemically (using L-Cysteine) and enzymatically (by papain) modification on the functional properties of hard wheat gluten. The modification process is run by both methods individually and synergistically. The results showed an increase in the degree of hydrolysis (DH) of lyophilized wheat gluten upon adding the reducing agent at different concentrations (50, 100, 150, 200, 250 ppm). The values for (DH) were (5.29, 6.18, 6.22, 6.26, and 7.48%) respectively. While for papain (2000 units / g protein) those values were (10.25, 12.71, 13.54, 13.88, 15.62%) respectively when the reducing agent (same concentration above) and papain were used synergistically. The functional properties (water holding capacity, emulsifying ability and stability, foam formation and stability, protein solubility, and oil holding capacity) of the gluten under study were examined. The results showed that the enzymatic modification significantly improved the functional properties of the experimental proteins compared to the reducing agent modification.

This study was aimed to determine whether chromium (Cr III) supplements have a beneficial effect on the insulin secretion and lipid profile of layers vaccinated against H5 avian influenza (AI). The study included serological parameter by ELISA kit and biochemical parameter for lipid profile test as well as to compare between highly pathogenic avian influenza (HPAI) recombinant (rH5N1) vaccine and classical (cH5N8) vaccine. The study was designed as follow: {G1: given rH5N1 vaccine only; G2: given cH5N8 only; G3: given rH5N1 with Cr III supplement; G4: given cH5N8 with Cr III supplement; G5: given Cr III supplement; G6: is negative control with no vaccination or supplementation}. Levels of insuline at 21 days old there were showed a significant increase in G3 and G5 as compared with another groups. At 42 days old, G3, G4 and G5 revealed the highest levels as compared with another groups. Similarity results for 60 days old groups. The results of lipid profile test at 60 days old showed the lowest level of cholesterol in G5 as compared to another groups and lowest levels of vLDL in G3, G4 and G5. However, there were no significant differences in triglycerides (Tri.Gs), HDL and LDL levels in all groups. In conclusion, Cr III supplementation can improve insulin secretion in layers vaccinated with AI H5 vaccines and it has a positive impact on lipid profile.

Streptomyces spp. is one of Actinomycete that produce variety of secondary metabolite (antibacterial, antifungal and anticancer product). Bacteria that used in this study was isolated from oily soil in Baghdad /Iraq. The morphological characters was gram positive, branching, spore former. The goal of this research was to find genetic polymorphisms of one bacterial isolate (assigned S1) and to assess the accurate phylogenetic distribution of this isolated based on ribosomal sequences. In this work one genomic region was amplified that covered a portion of the 16S rRNA sequences. According to the identified results, the currently investigated sample was positioned within the major phylogenetic clade of the Streptomyces albidoflavus sequences in the generated comprehensive tree. In conclusion, the present tree provided an inclusive tool for the confirmed identity of the sample under investigation As a result, the PCR-sequencing approach was used in the studied sample has presented a confirmed identity of this sample and showed the pattern of its phylogenetic distribution. This sort of observed diversity may suggest the possibility to utilize the current 16S rRNA sequences in the accurate detection and discrimination for the Streptomyces albidoflavus sequences.

This experiment was conducted at the poultry \ Animal Production Department - College of Agricultural Engineering Sciences - University of Baghdad, for the period from 22/11/2020 to 2/1/2021 . To study the effect of adding germinated red grains sorghum (GRGS) and raw red grains sorghum(RRGS) as a complete substitute for maize to broiler diets on production performance. 225 broiler chicks were used in this experiment and randomly distributed to five treatments by 45 chicks/treatment with three replicates/treatment , 15 chicks/replicate : T1 control treatment (maize) , T2 (20%) GRGS + (80%) RRGS , T3 (40%) GRGS + (60%) RRGS , T4 (60%) GRGS + (40%) RRGS , T5 (80%) GRGS + (20%) RRGS . the data was analyzed statistically according to complete randomize design (CRD) , register Qualities understudy over three periods 1 to 10 days a starter diet, and during the period from 11 to 24 days on a growth diet, and during the period from 25 to 42 days on a finisher diet according to the production guide of Ross 308. The results were showed significant superiority (P<0.01) of the T2 treatment as compared with other treatments for the body weight and weight gain and feed intake, a significant superiority (P<0.01) in the feed conversion ratio of the T1 treatment as compared with other treatments T2, T3, T5.

This study was conducted to evaluate the impact of two types of cryopreservation and different concentrations of Aloe vera as an antioxidant (0,10% and 20%) on the viability and normal morphology of immature and in vitro mature buffalo cryopreserved oocytes. The in vitro mature and immature oocytes were kept in cryopreservation solutions for rapid and vitrification techniques. The results revealed that the proportion of buffalo oocytes found to be viable and normal morphology were significantly (P<0.01) higher produced when using a high concentration (20%) of aloe vera on in vitro mature buffalo oocyte which is79.11% and 59.02%; respectively. We conclude from the present study that the viability and normal morphology of post-thawed buffalo cryopreserved in vitro matured oocytes were significantly (P < 0.01) higher produced in using a high concentration of aloe vera than other concentrations of aloe vera, from the side while with other side the vitrification technique than a rapid technique.

This study was conducted to evaluate the effect of two types of cryoprotectants and two cryopreservation methods on viability and normal morphology of the immature and in vitro mature of post-thawed buffalo oocytes. The in vitro mature and immature oocytes were kept in the vitrification solution (VS1) for 5 min and the vitrification solution (VS2) for 15 sec. The results revealed that the proportion of buffalo oocytes found to be viable and normal morphology were significantly (P< 0.01) higher in using glycerol which is 76.14% and 55.1%; respectively. We conclude from the present study that the viability and normal morphology of post-thawed buffalo vitrified in vitro matured oocytes were significantly (P < 0.01) higher in glycerol than those obtained in dimethyl sulfoxide (DMSO) and in vitrification technique than rapid technique.

This study was aimed to investigate the combined effect of flushing and hormonal treatment on reproductive aspects of local goats, 60 adult does were divided equally into two groups, the first was fed concentrate plus grazing commencing 3 weeks prior to mating and continue for 4 weeks post mating (flushed) F, whereas the 2nd group was raised on pasture only (P). All does were synchronized using impregnated with 40 mg medroxy acetate for 14 days. Following sponges withdrawn, the flushed and non- flushed does were then sub-divided in to four equal groups, the 1st flushed does were injected with 250 i.u hCG (T1), the 2nd only flushed (T2), the 3rd was only injected with hCG (T3) and the 4th was the control (T4). Results revealed that estrus response was significantly higher in T1 (100%), followed by T3 and T4 (86.66%) and T2 (80%). Fertility rate was significantly (P˂0.01) higher in T1 (80%) followed by T3 (73.33%), T4 (66.66%) and T2 (60.0%). Highest kidding rate (120.0%) and litter size (1.50) was noticed in T1, while the lowest kidding rate (86.66%) were found in T2 and T4. Insulin level was significantly higher in flushed does (6.04) compared to non-flushed does (2.81 uIU/ML).

This experiment was carried out to evaluate the effects of vitamin E (Alpha tocopherol acetate) supplementation and melatonin implants on growth performance, and meat quality traits of Awassi male lambs fed by a high and normal energy concentrated diet in Iraq. Lambs were divided equally to two main groups to be fed either high or normal energy diet ,and each of them divided in to five treatment, the first as a control (T1,T6),two level of melatonin (18, 36mg/lamb) were applied to (T2,T3,T7,T8) and two level of vitamin E (200,400mg/lamb/day) to (T4,T5,T9,T10).All animals were slaughtered at the end of the fattening period. Lambs received 400 g/day vitamin E with high energy diet surpassed significantly other treatment in final weight gain, vitamin E 400 mg/lamb/day and melatonin 18-36 mg/lamb at day 14 of storage increased retail color stability. enhanced meat color parameters. Melatonin implantation 18 mg/lamb, 36 mg /lamb significantly at day 7 of storage reduced MDA value, but at day 14 of storage vitamin E 200 and 400 mg/day/lamb reduced significantly MDA value of the Muscle Longissimus dorsi (LD) during storage.

Two field experiments were conducted during the spring season 2020 in Karbala governorate, to study the effect of sprinkler and surface drip irrigation systems to determine water consumption of potatoes and irrigation intervals using polymers and bio-fertilizers in desert soils. The experiment included three factors: 1-Irrigation system surface drip T1 and sprinkler T2, 2- The Irrigation interval: every 2 days I1, 4 days I2 and 6 days I3, 3- Addition of soil conditioners: control without any addition C, bio-organic fertilizers (seek) B, polymer (zeba) P, and polymer+ bio-organic fertilizers P+B. The experiment was designed according to the nested design with three replicates. Potato tuber class (Hermosa) rank E was planted. The results showed that the operating pressure of 50-150 kPa was drip and sprinkler irrigation, respectively. Irrigation interval treatment I1 also obtained the lowest added water depth of 212.64 and 486.70 mm at the drip and sprinkler irrigation systems, respectively. Moreover, the actual consumptive use ETa values for irrigation interval I1, I2, and I3 at drip irrigation were 276.44, 428.31, and 593.04 mm, respectively. Though at sprinkler irrigation were 550.50, 959.46, and 1385.08 mm for irrigation intervals I1, I2, and I3, respectively. The highest values of crop coefficient at tuber formation and filling stage were 0.66, 0.79, 1.06, 1.86, 3.42, and 4.73 for the irrigation interval I1, I2, and I3, respectively, at the drip and sprinkler irrigation systems.

This study was investigated the P transformation in three soils gathered from different locations (Agricultural researches center in Tel Afar, Ain-Talawi and Sinu in Singar) in north of Iraq representing different gypsum content and land use [wheat (W), vegetable (V), uncultivated (Un)] after incubation period of (0, 5, 10, 30, 60, and 90) days. The soils were treated with 200 mg P kg-1 as superphosphate (S) or 30 g kg-1 soil manure as a cattle manure (A) or compost (p). The appropriate amounts of water were added to bring soil to estimated field capacity. After the specified time of each incubation period, sub-sample of soil analyzed for P, pH, EC and Ca. The results showed that P status affected by amendments and incubation periods. The soil which has more available P was the soil which treated with cattle manure. Generally, P was more available under (V) use compared to (W) use. After 90 days of incubation P solubility generally was higher than after 30 days. It appears from P solubility diagram that the best treatment was (A) which almost located between the two more soluble P compounds DCPD and DCP.

This research aimed to measure and analyze the impact of exchange rate shocks on some variables of the Iraqi economy during (1990-2022), because of the different effects of these shocks on the macroeconomic variables represented in money supply and agricultural output. Exchange rates are related to the policy chosen by the Central Bank of Iraq in managing the foreign exchange market and in the rentier nature of the Iraqi economy. The research uses a quantitative method in estimating the levels of the impact of exchange rate shocks on some economic variables. Several methods are conducted to achieve the goals, including the VAR model, variance decomposition and Impulse Response Functions. The results showed that exchange rate variance reached 100% in the same variable in the first year and decrease to 97% at the end of the period, and the same in the impulse response. It was an internal reaction that predicts what can be called the self-wave of an exchange rate rise, while both the variation and the response to the money supply shock in Iraq were dependent on the policy of the Central Bank and compatible with what was happening in the exchange rate, as the analysis of variance in the first year reached 38.98% for the same variable and 61% of it is due to the exchange rate. The results also showed that agricultural output was weakly affected by the exchange rate shock and money supply.

This study was aimed to investigate the potential adsorptive behavior of mixed algae for decolorization of methylene blue dye from aqueous solution in the form of free and immobilized. Effects of initial pH value (3-9), biosorbent dosage (0.02-0.5)g/L, and initial concentration (10-50) mg/L for different contact time (0-180) min at 200 rpm shaking speed were investigated. In addition, experimental data were analyzed using adsorption (Langmuir and Freundlich) isotherms as well as kinetic models. The results demonstrated that the maximum decolorization percentage was 93% and 91% by free and immobilized algae, respectively for pH 6, 10 initial concentration, 0.3g/100mL algae dosage, and 90 min contact time. The results revealed that the Langmuir isotherm model was adequate for describing the dye removal process (R2= 0.99) for both forms. Results of pseudo-second order kinetic model revealed best fitting with the sorption data indicating that chemisorption process is the dominant mechanism controlling the dye removal. Furthermore, the characterization study performed using FT-IR and scanning electron micrograph techniques, revealed that the used algal biomass has good biosorption capabilities associated to active groups and surface structure and confirming that the immobilization was successful.

The experiments were carried out at the College of Agricultural Engineering Sciences - University of Baghdad during the fall season of 2021-2020 to study the effect of foliar application of ascorbic acid (0, 25, 50, 75 mg. L-1) which were symbolized as A0, A1, A2, and A3, and chelated calcium (0, 1.0, 1.5, 2.0 g. L-1) which were symbolized as Ca0, Ca1, Ca2, and Ca3 on the growth, yield and marketability of broccoli. A factorial experiment was carried out within the RCBD design, in a total of 16 treatments with three replicates. The main flowering heads were kept at temperatures 6-8° C for 20 days and 18-25° C for 4 days. The results were revealed that the individual and interaction treatments were recorded a significant values among the studied parameters, and the interaction treatment of A3Ca3 achieved the most significant increment of leaves area, total yield, heads weight, heads diameter, calcium content in leaves and heads, the chlorophyll and vitamin C content in the heads, 1962.54dm2.plant-1, 55.10 ton.ha-1, 889.78 g.head-1, 21.84 cm, 1.85% in leave, 1.68%, 152.35 mg.100g-1 and 305.07 mg.100g-1 respectively decreased weight loss, increased total soluble solids, and maintained chlorophyll and vitamin C in the flowering heads after storage reached 11.40%, 134.72 and 96.41 mg.100g-1, 136.40 and 130.53 mg.100g-1 respectively at two temperatures.

Levofloxacin (LEV) is an important and widely used antibiotic. It is used to treat many bacterial infections. It may be found in water sources as a result of incomplete metabolism in humans and other sources. In this study, the adsorption of Levofloxacin by raw rice husk (RRH) was investigated. The effect of pH, time, temperature, RRH weight, and initial Levofloxacin concentration on the adsorption process were determined. Kinetic, isotherm and thermodynamic models were studied to explain the Levofloxacin adsorption mechanism on raw rice husk. The results of adsorption kinetics and isotherms revealed that the adsorption of LEV by RRH was better fitted with the Intraparticle model with coefficient of determination (R2=96%), while the Langmuir model represents the best isotherm model to describe the adsorption process with (R2 =95%). The removal efficiency reached 99% , the monolayer maximum uptake qmax is 2.47 mg/g and the adsorption intensity parameter (1/n) value is 0.763, which means that the adsorption is favorable. According to the thermodynamic coefficients, the adsorption process was spontaneous and feasible for the temperatures under investigation; the sorption efficiency was more favorable at higher temperatures, and the adsorption process is endothermic.

A field experiment was conducted in the experimental field of the Crop Sciences Department at the College of Agricultural Engineering Sciences - University of Baghdad during fall seasons of 2021 and 2022. The aim of this study was to investigate the role of nano and metallic boron foliar nutrition on yield, components, water use efficiency, and water consumption under water stress for sweet corn (Zea mays L.). Randomized Complete Block Design was used within split -plot arrangement with three replicates, where the main plots included three levels of water stress (irrigation at 40, 60, and 80% of available water) coded as W1, W2, and W3, respectively. The nano and metallic boron spray concentrations represented 5, 10, 20, and 40 mg L-1 coded as N1, N2, M1, and M2, respectively. Results showed that nano and metallic boron concentrations significantly affected all the studied traits. The concentration of 5 mg L-1 of nano boron N1 significantly exceeded other concentrations under study in increasing ear length, number of rows per ear, number of grains per row, and weight of 500 grains, which positively reflected on improving grain yield of 5.93 and 5.96 t ha-1. The interaction between water stress treatments and nano and metallic boron concentrations was significant for all the studied traits except for ear length.

This study was aimed to convert agricultural waste to organic fertilizer and find the best combinations from agricultural waste, that were used in the research, Composting has always shown to be a reliable and ecologically beneficial technique for removing large amounts of bio-waste. Bio-waste is produced in a variety of sorts (forest residue, farms, agricultural, food industry, and municipal garbage) and is expanding on a daily basis, offering a management and disposal challenge. Millions of tons of cereal vegetable crops, industrial crops, dates and fruits, are produces in Iraq, there are about 20 million tons per year of agriculture waste materials in Iraq, according to estimates, and only a small part of it is used. Composting is an alternate method of material recovery that has no negative consequences. The composting was performed for study with agricultural waste from tree leaves, twigs, date palm, rise husk as a carbon source and manure (sheep, horses, poultry), and vegetable and fruit market waste as a nitrogen source to determine the appropriate mixing, proportion, and combination, as well as the influence of sludge on mixtures. Six distinct experiments were conducted to investigate the alterations in physic-chemical and biological changes during composting utilizing the rotary drum method. Consequently, the compost generated by all experiments had a pH of 6.95–8.00, electrical conductivity of 1.42–2.03 dS.cm⁻¹, a drop in the percentage of overall organic carbon of 16.4–13 percent, a percentage rise in total nitrogen of 0.86–0.19 percent, and a C:N ratio of 8.8–13.7. In this research, the optimal proportions from the various combinations attempted were discovered in drum (B2).

This research aimed to illustrate the significant role of modern technology packages in agriculture, in particular in the growth of wheat cultivation. In this research will attempt to use O-Ring method. The purpose our using of this method is to prove that the modern technological packages used in agriculture, wheat cropping combined have a significant impact on increasing the productivity of the wheat crop in Iraq. A sample is taking from 290 farms, by divided into three governorates Namely Wasit, Babil and Diwaniyah. The packaging used to grow wheat was micronutrients, seeds, fertilizers, laser-level potassium sulphate, seeds, potassium sulphate fertilizers, Pallas herbicide, Atlantis herbicide and the farming cycle. All these elements were expressed; as A3i. This is an indicator of the intensification of agricultural inputs for developing wheat cultivation in Iraq. The results of the analysis showed that there is a high degree of correlation between each of the modern technological inputs and the rest of the modern inputs. The results also indicated that there is a growth and development in the productivity of the wheat crop because of the use of modern technologies, as there is a correlation between the use of the modern technology package and the development of the productivity of the wheat crop in Iraq.

This study was aimed to determine the best nutrient balance for chickpeas from the relationship between the Norms of nutrients with the critical point for each nutrient using the DRIS system. This experiment was carried out at the Grdarasha farm, college of Agriculture Engineering Science, University of Salahaddin during the spring growing season of 2020, to find out the effects of three levels of nitrogen (UREA) (0, 15, 30 Kg N ha-1), four levels of triple super phosphate (0, 20, 40, 60 Kg P ha-1), and three levels of KCl fertilizer (0, 15, 30 Kg K ha-1) and their combination on Chickpea yield and nutrient balance by using Split Split plot design with three replicates. The highest yield (1.55 Mg ha-1) was recorded from the lowest (2.02) AT or NBI (nutrient balance index), while the lowest yield (0.73 Mg ha-1) was obtained from the highest AT value (62.68).

This article investigates the ability of two types of mealybugs, citrus (Planococcus citri) and longtailed (Pseudococcus longispinus) mealybugs, of acquiring and transmitting Grapevine vein-clearing virus (GVCV) in a greenhouse setting. Mealybugs are the primary vectors for most Badnaviruses, and only a few species have been shown to be aphid-transmitted. In this study, we tested the acquisition and transmission ability of two mealybug species using GVCV-infected and healthy grapevines in a greenhouse setting for three consecutive seasons. This study determined that acquisition time by the mealybugs could be as low as three days, yet the transmission of GVCV from infected grapevines to healthy grapevines by these two mealybug species was unsuccessful. Additionally, with the use of previously-developed species-specific primers, this study determined that those mealybugs captured in the greenhouse facilities at the University of Missouri could not be identified using these primers, and required primers that were specific to their regional diversity. This study contributes to the wider understanding of the acquisition and transmission of GVCV by certain mealybug species.

This research was carried out at University of Baghdad - College of Agricultural Engineering Sciences during the fall season of 2020 and spring season of 2021 in order to evaluate the effect of organic fertilizer and the foliar application of boron on the growth and yield of industrial potatoes (Solanum tuberosum L.). Using factorial experiment (5*4) within Randomized Complete Block Design with three replicates, the organic fertilizer (palm fronds peat) was applied at four levels (0, 12, 24, and 36 ton ha-1) in addition to the treatment of the recommended of chemical fertilizer. The foliar application of Boron was applied at four concentrations which were 0, 100, 150 and 200 mg (H3Bo3). L-1. The results Revealed a significant increase under the application of organic fertilizer at the level of 24 ton.ha-1 and the foliar application of boron at a concentration of 100 mg. L-1 (O24B100) on plant height, main branches number, and leaves number, which was reflected on the increase of vegetative dry weight and then the yield of potato (63.67 cm and 57.00 cm plant-1), (4.89 and 5.22 stem. plant-1), (66.50, and 55.67 leaf. plant-1), (113.38 and 108.49 g. plant-1), (44.17 and 59.48 ton.ha-1) for both seasons, respectively. It also recorded a Significant differences in the chemical parameters in the leaves, as Chlorophyll reached (265.51 and 203.12 mg.100 g-1 wet weight), N (4.883 and 2.293%), P (0.577 and 0.413%), K (2.467 and 2.660%) and B (65.60 and 63.56 mg. kg-1) for both seasons, respectively.