Al-Qadisiyah Journal of Veterinary Medicine Sciences

This research includes the histological study of the spleen in the native, wild duck Anas platyrhynca especially presences and distribution of T-cells and B-cells in the spleen tissue. (10) Healthy birds of duck were used, (5) males and (5) females. This study clarifies that the spleen of a duck consists of two distinct regions: white pulp and red pulp, the white pulp form the major part of the organ, it’s a lymphoid tissue that could be differentiated into (2) types: diffuse lymphatic tissue and nodular lymphatic tissue which found as two distinct forms of nodules .the first nodule revealed to be regular and ellipsoidal in shape, small in diameter, surrounded with its eccentric artery by a thick layer of connective tissue capsule and show the low percentage, which is B-cells. The second nodule is irregular and oval in shape, large in diameter, has a thick capsule thinner than the last capsule, without artery inside or outside the nodule, and represented a high percentage so it distributes in almost the spleen, which is T-cells.

Bovine papillomatosis (BP) is conveyed worldwide among cattle, however, is moderately less regular in water buffalo. The point of the present work was Molecular detection of cutaneous fibro papillomatosis in Buffalo in Al-Qadisiyah province, Iraq. Forty cutaneous warts (CW) samples were collected from lesions distributed on any part of the body (shoulder, thigh, around the eye region, and in the perineal region) of buffaloes in different ages. Out of 40 samples subjected to PCR, 26 samples were positive for BPVs. From the 26 positive samples, 4 samples were sent for DNA sequence analyses and found two samples was positive for Deltapapillomavirus (BPV-1) and two samples positive for Xipapillomavirus (BPV-4). The sequencing analysis for the BPV L1 fragment was identified from the CW that shown a 100% identity with BPV-4 from Iraq (KY662042.1_1) and BPV-1 sequences from Poland (KX594402.1 and KF284141.1). The molecular and phylogenetic results of the current study reported the similarity and close relation between the BPV-1 and 4 genotypes of bovine and buffalo in Iraqi breeds and other BPV strains from other countries.

This study was conducted to investigate the role of L.Carnitine in enhancing the developmental progress of in-vitro produced bovine embryos. A total of 794 cumulus-oocyte complexes (COCs) were collected by the slicing method from the genitalia of unknown age and breed slaughtered cows. The COCs were divided into four groups and matured in TCM-199 supplemented with four concentrations of L.Carnitine (0.0mg/ml, 0.3mg/ml, 0.6mg/ml and 1.2mg/ml), a concentration for each group. The results showed that the groups treated with 0.3 and 0.6 mg/ml recorded higher percentages of oocytes maturation (48.98% and 49.20%) respectively with significant (P<0.01) differences compared with control and 1.2mg/ml. Matured oocytes were fertilized by straws of frozen-thawed semen, and the resulted embryos were divided into four groups and cultured in media supplemented with the four concentrations of L.Carnitine (0.0mg/ml, 0.3mg/ml, 0.6mg/ml, and 1.2mg/ml) in order to investigate its effect on embryos developmental progress. Results showed that the concentrations (0.3 and 0.6 mg/ml) gave better percentages of embryo development (18.55% and 17.20%) respectively with significant (P<0.05) differences when compared with control and 1.2mg/ml. In conclusion, supplementation of in-vitro bovine oocytes maturation media and in-vitro embryo development media with 0.3 and 0.6 mg/ml of L.Carnitine improved significantly oocyte maturation and embryo developmental progress.

The study was achieved on 16 local Arabian horses and 16 local donkeys. The aim of the study was to compare of some blood and synovial fluid biomarkers of local Arabian horses and donkeys. Blood analyses were done to measure PCV, Hb, WBC counts, and RBC counts. Synovial fluid analysis was done to estimate some physical and biochemical markers, total protein, glucose, alkaline phosphatase, urea, creatinine, creatinine kinase, cholesterol, uric acid. The differences of the mean± SE of PCV and Hb values were non-significant at P≤0.05. Whereas the variances of the mean± SE of WBC counts and RBC counts were significant. The physical markers of the synovial fluid samples were clear for both horses and donkeys nevertheless the volume was greater and more viscous for horses with straw color though it was pale amber for donkeys. The inconsistencies of the mean± SE of total protein, and uric acid were not significant at P≤ 0.05. But the variances of the mean± SE of glucose, alkaline phosphatase, urea, creatinine, creatinine kinase, and cholesterol were significant.

Propolis is a complex compound and important natural bee product that belongs to the great family of bee products with variety in color, chemical composition as well as in valuable biological and pharmacological effective. To date, No data about the Iraqi specified chemical constituents of Al-Diwanyiah propolis have been reported. The present data was investigated the phytochemical components of the ethanolic extract of collected propolis samples from varies honey bee colonies via distinguished fraction employing GC-MS technique. The chemical compounds were determine and identified by comparison with mass spectra library of the GC-Mass data system and with literature mass spectra. The results exhibited the total extract yields ranged from 38.6-54.4 % with mean percentage equal 45.7%. A fifty one different phytochemical metabolomic compounds were tentatively identified in local propolis samples belong to the at least twelve phytochemical categories. The major phytochemical categories were flavonoids, alcoholic compounds, fatty acids and their esters, terpenes, aromatic acids, aldehyde compounds, aliphatic hydrocarbons, aromatic hydrocarbons and other categories were esters, sugar and metabolic derivatives, dicarboxylic acid. The predominant flavonoids identified in propolis under test were Pinocembrin and tectochrysin. The present study showed local propolis is a rich source of natural bee active substances for identified profiling metabolomic markers biological and finalized therapeutic remedied applications in different pharmaceutics as well as GC-Mass is a good technique to provide an overall view of propolis composition.