Rafidain journal of science

The transfer ability of antibiotic resistance plasmid DNA in the bacterial species Escherichia coli, Klebsiella oxytoca, Salmonella spp., Enterobacter cloacae and Morganella morganii which previously isolated from patients suffering from diarrhea, has been examined via conjugation. Plasmid DNA from the first four species carrying resistance for ampicilin, chloramphenicol, gentamycin, erythromycin and tetracycline and for heavy metals (mercury chloride, cadmium chloride and silver nitrate) was found capable of transfer to the laboratory E. coil strain (JM83) by conjugation which became resistant to these antibiotics and drugs The plasmid DNA transfer has been confirmed by analyzing the plasmid DNA content of the JM83 transconjugant colonies using agarose gel electrophoresis where the plasmid profile revealed the existence of the transferred DNA plasmid in the recipient JM83cells.

Four types of copolymers have been prepared by solution condensation polymerization of azomethine monomer with four different types of acid chloride (phthaloyl, isophthaloyl, terephthaloyl and fumaryl).
The electrical conductivity of the copolymers have been enhanced by many order of magnititude according to polyazomethine or polyester. The ester group increased the electrical conductivity via two manners, increasing the flexibility of the chain, and making the charge carrier movement more easily along the chain.
The effect of temperature and doping with iodine on conductivity have been investigated. It was found that the electrical conductivity of these types of polymers is deffected by the distortion of the phenyl ring and the flexibility of the chain.
Key words: electrical conductivity, polyazomethine, polyester

A sensitive indirect spectrophotometric method is developed for the determination of bromhexine-HCl in pure and dosage forms. The method is based on the oxidation of bromhexine-HCl with iron(III) in acidic medium, then reaction of iron(II) with 1,10-phenathroline to produce a ferroin complex which is water-soluble, stable, and has a maximum absorption at 510 nm against the reagent blank with a molar absorptivity of 3.51×104 l.mol-1.cm-1. Beer’s law is valid over the concentration range of 10 to 220g bromhexine-HCl in a final volume of 25 ml. The proposed method has been applied successfully to determine bromhexine-HCl in different pharmaceutical preparations

A simple, rapid, accurate and precise spectrophotometric method is proposed for determination of pyridoxine hydrochloride (Vitamin B6) in pure form and in its pharmaceutical preparations and serum. The method is based on the oxidation-reduction reaction between vitamin B6 and cerium (IV) ion (ceric ion), then the subsequent reaction of cerium (III) with arsenazo III reagent in acidic medium in the presence of the neutral surfactant (Triton X-100) to produce a green complex which is stable, water soluble and has a maximum absorption at 716 nm with a molar absorptivity of 1.12105 l.mol-1.cm-1. Beer’s law is obeyed in the concentration range of 1 to 14 g of vitamin B6 in a final volume of 25 ml. The proposed method has been applied successfully to the assay of pyridoxine hydrochloride in pharmaceutical preparations and serum.

A number of Pd+2 and Pt+2 complexes with 2-methyl-3-aminoquinazoline 4-one (L1) and 2-methyl-3-hydroxyquinazoline-4-one (L2) have been synthesized by direct reaction of Na2PdCl4 or K2PtCl4 with the ligands. Complexes of the general formula [M(L)(SC6H4.p-Me)2] were also prepared through neucleophilic substitution reaction. The prepared complexes were characterized by IR and UV/Vis spectroscopy, conductivity, magnetic measurement and the metal content were determined spectrophotometrically. The electronic spectra and magnetic susceptibility indicates that the complexes have a square planer geometry in which the ligands behave as bidentate

In this research, xanthine oxidase (XO) was partially purified from sera of patients with gout, by dialysis and anion exchange chromatography techniques. One protein peak of XO activity was obtained with specific activity (0.149) unit/mg protein and with purification fold of (496.66) compared to crude enzyme.
Inhibition of partially purified XO was studied by allopurinol, caffeine and allopurinol with caffeine as a prodrug. The lower concentration of each inhibitor which exhibited maximal inhibitory effect was (10-7) mM. The inhibition type of XO by using these inhibitors at these concentrations competitive inhibition.
The results show that Km value without inhibitors was (40) mM. However, was Km values were (58.8), (100), and (125) mM with inhibitors respectively, and Vmax value remained stable (0.135) unit/ml. The inhibition constant Ki values were (0.68), (0.4) and (0.32) mM, respectively.
Accordingly, these results indicate that inhibitory effect of allopurinol with caffeine was better than caffeine, and caffeine was better than allopurinol. We suggest the intake of herbs and plants containing caffeine alone and/or with allopurinol drug to prevent or treat gout

Biochemical studies showed the presence of various significant distinctions in the activity of enzymes in some types of cercariae under study. The highest activity was (4.90 0.26) nmol/min/mg protein for adenosine deaminase in monostome cercaria, (297.30 7.99) mol/min/mg protein for glutathione –S- transferase in microcercous cercaria, (209.40 6.05) mol/min/gm fresh weight for acetylcholinesterase in furcocercous cercariae, (94.67 1.15) enzymatic unit for lactate dehydrogenase in gymnocephalous cercaria. Finally, (23.40 1.08) enzymatic unit for alkaline phosphatase and (7.27 0.25)enzymatic unit for glucose-6-phophate dehydrogenase in distome cercaria. Therefore the activity of these enzymes could be used in distinguishing the various types of cercariae.
Electrophoresis technique showed different patterns of bands. Approximate molecular weights for protein bands in various cercariae varied between (25000-676000) Dalton.

This study involved testing the effect of four subinhibitory concentrations of garlic extracts (aqueous and alcoholic) on the mutagenicity of short wave ultraviolet
(UVC 253.7 nm) in conidia of the fungus Aspergillus amstelodami.
The concentrations of the aqueous extract tested were 2.5 , 5 , 7.5 and 10 mg / ml conidial suspension, The concentrations of alcoholic extract were 0.5 , 1 , 1.5 and 2 mg / ml conidial suspension. Tests were done using the pre-treatment method. Both the aqueous and alcoholic extracts caused inhibition in the mutagenicity of UVC in conidia of the fungus A. amstelodami.

The research dealed with the study of the alkaline phosphatase, 5-nucleotidase and acetyl choline esterase activity in the blood and the synovial fluid of the Rheumatoid arthritis patients. The enzymes activities of the blood of Rheumatoid patients and healthy people have been compared, and of both sexes. Also, a comparison of these activities in the synovial fluid and the blood in 5 patients has been conducted.
The results in the blood showed an increase in the activity of alkaline phosphatase in male and female patients in different percentages. The activity of 5-nucleotidase remained with in its normal value in both sexes in all age groups, while the activity of acetylcholine esterase decreased in male and female patients in all age groups compared to healthy peoples.
The study of the synovial fluid showed a decrease in alkaline phosphatase and acetylcholine esterase enzymes activity while an increase in the 5-nucleotidase enzyme activity compared with that in blood for the same patients.
Therefore, the activity of the acetylcholine esterase. could be used as new marker for diagnosis rheumatoid artheritis in addition to the existing means.

The present study surveyed the intestinal parasites which cause diarrhea in 600 children consulting Ibin-al_Ather hospital in Neinavah governorate for a period of 10 months using both direct smear and concentration technique for examining stool. The total percentage of infection was 64.16% among those referred to the laboratory for stool examination. Four species of parasites were recorded: two species of protozoa, namely: Entamoeba histolytica 36.10% and Giardia lamblia 40.26% and two species of worms namely Hymenolepis nana 19.74% and Enterobius vermicularis 3.89%. Also the study extended to reveal the factors that affect the percentage of infection and symptoms associated with diarrhea.
The highest percentage of infection was in the age 1-2 years and the males had higher infection rate than females and children coming from rural regions more than those coming from urban region

The inhibitory effect of propolis produced by honey bees was determined for some Gr positive (Staphylococcus aureus, Streptococcus pyogenes, Streptococcus faecalis) and some Gr negative (Klebsiella pnemonia, Salmonella typhimurium, Proteus vulgaris, Pseudomonas aeruginosa, Escherichia coli.), the inhibition was particularly evident on, Staphylococcus aureus, Streptococcus pyogenes and Klebsiella pneumonia. The minimum inhibitory concentration (MIC) of porpolis on these three bacteria were determined. These were 2.5 (µg/disc) for Streptococcus pyogenes, 0.25 for Klebsiella pneumonia and Staphylococcus aureus

Larvae of Contracaecum sp. were found encysted in the stomach of Silurus glanis in three successive stages namely L2, L3 and L4. The cystes were white in color, elongated and their dimensions were 0.5×0.9 mm. In general, the wall consists of two layers, outer thick and inner thin layers. The outer one consists in turn of collagenous fibers intermingled with epithelial cells, fibroblasts, macrophages and lymphocytes. While the inner layer consist of collagenous fibers, few fibroblasts, lymphocytes and fibrocytes. The most important histological changes due to the infection with this larva is infiltration of inflammatory cells around the cyst and its cavity , which is represented by macrophages, nucleated cells and acidophils, and in same time occurrence of necrosis, and local aggregation of lymphocytes between the muscle bundles. Also, few pycnotic cells were observed and erythrocytes, macrophages and remnants of cells around the larval cuticle, and the presence of blood vessels surrounding the cyst. Furthermore, dwarfness of intestinal glands and destruction of the epithelial tissue were observed.
As regard the carbohydrate, the cyst walls reacted strongly positive with Periodic acid Schiff, Alcian blue and Best Carmine, while the cyst layer reacted negatively with Toludine blue.
Both cyst walls reacted positively with protein detecting technique including Mercury Bromophenol blue and Ninhydrin Schiff, with lipid detecting technique such as Aceton Sudan Black–B, Feulgen reaction, for detecting DNA, finally, strongly reacted by using Von Kossa technique

This study was conducted to investigate the effect of Zingiber officinale rhizomes powder in some physiological, histological and biochemical features (7-8) months old in white male rabbits, Rabbits were divided into 2 groups (6 rabbits for each group). The first group was given the standard ration and normal water, the second group was given Zingiber officinal powder (1000 mg/kg) ration daily for 4 weeks. Results showed that the treatment with Zingiber officinale's powder caused a significant decrease in glucose, cholesterol and triglycerides a swell as a significant decrease in lowdensity lipoprotein LDL-C level, and increase in highdensity lipoprotein HDL-C level in blood serum, as well as increase of testosterone hormone level in comparison with control group. Histological sections showed some changes of liver tissue as swelling of hepatic cells, enlargement of centeral vein diameter. The testes showed increasing of cell layers lining the seminiferous tubules and increasing of leydig cells number. The adrenal gland showed an appearance of large sinusoids in each of Zona - Fasiculata and Zona – reticularis of the cortex.

The effect of four plant extracts, mixed with diet of the 3rd instar larvae of Musca domestica L., on the growth and development were studied and the results revealed that , Azadirachta excelsa Jack leaves extract at 400 ppm concentraion caused 94% mortality after 7days of treatment in larval, pupal and adult stages, in addition to causing morphogenetic aberrations in pupae and adults, similar to that caused by insect growth regulatores. Pimpinella anisum L. seed extract (400 ppm) caused 50% mortality after 7 days in larval, pupal and adult stages. Bark extract of Cinnamomum zeylanicum L. had a low effect (40% mortality) in larval, pupal and adult stages, which Solanum nigrum L. leaves extract had no effect.

The ability of the fungi Trichoderma pseudokomngii, Trichoderma
harizianum and Gliocladium roseum to produce Polygalacturonase (PG),
Polygalacturonase transeliminase (PGT), Carboxy methyl cellulase (CMCase) and protease were studied in culture medium filtrates as well as mycelial mats. The study revealed that (PG) in the culture medium filtrate of each fungus had higher activity than in that in the mycelial mat. While there is no significant difference in it's activity between the culture medium filtrate and mycelial mat in the three fungi (PGT) activity of
T. harizianum culture medium filtrate had significant difference from that of the other fungi wheares (PGT) had significant difference between G. roseum culture medium filtrate and it's mycelial mat. While T. pseudokomngii had the reverse. The results revealed that there is no significant difference in the (CMCase) activity of the culture medium filtrate for T. pseudokomngii and T. harizianum. Also no significant difference has been found in the enzyme activity of the mycelial mat for the three fungi. The enzymeprotease had higher activity in the G. roseum culture medium than of
T. pseudokomngii and T. harizianum .

The parent seeds of Six durum wheat (Triticum durum Desf), (1-Azul-5, 2-Yousef-1, 3-Omgenil-3, 4-Syrian-4, 5-Mrb3/mna-1 and 6-Um-Rabee) and their half diallel crosses were planted in the field of College of Agricultural and Forestry at Hammam AL-Alil during the season 2001 – 2002 using randomized complete block design (R.C.B.D.) with three replications to study the effects of general and specific combining abilities for number of days to 50 % flowering, plant hight, number of grains/spike, number of spikes/plant, Biological yield, grain yield, harvest index, 1000-grain weight and protein percentage. The genotype mean variance (parents and F1) was significant at probability level of 1% for all the traits. General combining ability variance was significant at 1% probability level for all the traits except for 1000-grain weight where as specific combining ability variance was significant at 1% of probability level for all the traits except for number of days to 50% flowering which was significant at 5% of probability level. The ratio of the general combining ability to specific combining ability components was more than one for all the traits except for number of spikes / plant, 1000-grain weight and protein percentage. General combining ability effects for Syrian-4 and Um-Rabee parents were in desirable direction for most traits. The cross Azil-5 × Um-Rabee exhibited desirable effects for general and specific combining ability for all the traits except for the harvest index and protein percentage

The study included the isolation and identification of the contaminating bacteria of the local cheese in Mosul as (110) samples of cheese were collected from the local markets. The study showed that the contamination rate was (100%) most of them were related to enterobacteriaceae which represent (80%). (16.66%) were related to Staphylococci, while Listeria mocnocytogenes represent (1.7%), Brucella melitensis forming (1.7%), among the enterobacteriaceae were (3.1%) Yersinia enterocolitica. The ability of some pathogenic bacteria transmitted through contaminated cheese to produce enzymes as Lecithinase, Lipase and Protease. The results showed the ability of
L. monocytogenes to produce Lecithinase, Lipase and Protease. Br. Melitensis isolates were unable to produce them, Y. enterocolitica isolates were capable of producing Lipase only.

In a study dealing with the occurrence of Ligula intestinalis larvae in 344 (Acanthobrama marmid) (Heckel, 1843) fish during the period from Aug 2000 to March 2001, a total infection rate of 24.12% was observed. The infection rates in male and female fish were 21.25%, 26.63% respectively. The number of the larvae ranged from
1-7 perfish.
The total average length of the obtained larvae were 26.7 cm. The infection fish suffered from genital organs atrophy.

The effect of hibitane and formalin was studied against Pseudomonas aeruginosa. Bacterial isolates were taken from respiratory, urinary tracts and wound infections.
The sensitivity percentages were recorded to different concentrations of disinfectants in different time intervals. The study showed that Hibitane has a better effect on this bacterium than Formalin, where the isolates were (70%) sensitive to concentration
(2000 g\cm3) of Hibitane after (40) minutes of treatment, while their sensitivity percentage to the same concentration of Formalin was (41.7%) after (60) minutes of treatment. The study also showed that sensitivity percentage increases with the increase of disinfectant concentration and treatment time

The research included a study of (50) samples of blood serum healthy control compared
with (50) samples of atherosclerotic patients. It was found that the normal range of arylesterase activity in control group (117.22 ± 5.03 U/ml), The enzyme activity was not affected by sex and decreased with aging. Also sex and age had no effect on the enzyme activity in patients. A significant decrease in activity, HDL, total protein, albumin and globulin, was found also that a significant increase of total lipids, where as no change was found in total cholesterol, triglyceride, VLDL, LDL and calcium in serum of atherosclerotic patients compared with control group.
Correlation coefficient between arylesterase activity and the clinical variables in control and patients groups showed a linear relationship of arylesterase activity with total protein in control group, with HDL and albumin in patients group.

The research included isolation and purification of arylesterase from normal blood serum. The molecular weight of the partially purified enzyme was determined by gel filtration using (Sephadex G75) and SDS electrophoersis techniques. It was found that the first proteinous peak separated from ammonium sulfate saturation of serum with (25 50%) using gel filtration and SDS electrophoresis techniques had apparent molecular weight of (48123 d) and (49185d) respectively.The second proteinous peak separated from serum by gel filtration technique had apparent molecular weight (47305 d).On the other hand, the first proteinous peak separated from HDL serum had a molecular weight (49907 d).
The optimum conditions of arylesterase for the two peaks separated from HDL and ammonium sulfate precipitaion solution showed an optimum reaction incubation time at (12),(10) minutes, an optimum pH at(7.2),(7.6), an optimum temperature(30 C), (25 C) and optimum substrate concentration (4 mM), (4.5 mM) and Vmax value (243.9U/ml), (238.1 U/ml) and Km value (3.07 mM), (3.26 mM) respectively.

Rheological properties of qaiyarah asphalt were modified by air blowing for different intervals of time. Sulfur has been added in different wt. percentages (5,10,15) to the oxidized asphalt.The homogeneity of samples was studied after more than one year of aging.The results of microscopic tests showed improvement in the homogeneity of these blends in comparison with the original asphalt- sulfur blends.

One of the major problem for the toxic Goiter is the estimation of absorbed dose, because the increase or the decrease in the dose negatively affects the treatment of the disease. In this research a method was proposed to measure the radiation dose for the enlarged region for some of the diseases of the toxic goiter which is common in the northern part of Iraq using (TLD) dosimeter of (LiF) which can be used generally to measure the radiation dose. It is used here for its great convenience for this disease. The dosimeters are fixed on the enlarged region of the goiter then the patient takes the dose of 131I. The proposed method for measure the radiation dose depend on the analysis of decay scheme of 131I and counting the different emitted energies and its percentages combining this theoretical analysis with the reading of the (TLD) the values of the absorbed dose by the enlarged regions. In this way for measuring the absorbed dose the physician can estimate the future dose

The background radioactivity of environmental Nineveh governorate was determined by measuring the radioactivity for each one of cesium Cs137 and potassium K40 in soil, plant and milk using highly parity germanium (HPGe) detector with crystal volume (76 cc) and energy resolution (2.2 keV) at the gamma line (1332 keV) of Co60 radioactive calibration source.
The measurements and sampling procedure cover (41) positions from different parts of Nineveh governorate, the results shows that Cs137 specific activity range is:
(15.89 – 70.97) Bq/kg in soil, (1.1 – 3.73) Bq/kg in plant and (o.63 – 2.1) Bq/kg in milk.
While the K40 specific activity range is: (323.86 – 1025.0) Bq/kg in soil,
(210.51 – 599.134) Bq/kg in plant and (200.25 – 480.1) Bq/kg in milk.
It is noted that the results in this work shows good agreement with other literatures and the activity levels are within the reasonable values denoted by authorized agency, as well as the fluctuations in results may be due to the geological and geographical differences.