Abstract
Tropical bovine theileriosis was recognized as one of the most important cattle diseases in Iraq. The target purpose from present study is to detect Tropical bovine theileriosis among carrier cattle in Al- Nasiriyah city by using each of PCR technique and blood smear method . Out of 60 Blood samples, Blood smear examination revealed 40 (66.67 %) samples to be positive for intra erythrocytic Theilera when staining with Geimsa stain .The specificity of the T. annulata primer for cytob1 gene was examined with DNA fromT. annulata, the expected 312-bp was generated from T. annulata DNA. The cytob1 gene was detected in 48 samples (80%), while in negative results in 12 samples (20%). The sensitivity of PCR techniqueis greater more than light microscopy examination.
Keywords
Blood smear
cattle parasites
protozoa.
Theileriosis