Rafidain journal of science

The study included the effect of three compounds of folate analogues,
sulfanilamide, trimethoprim and aminopterin on the growth of seedlings and callus of
Nigella sativa plant. These compounds were added at concentration of (10-1-10-5) Molar.
The average roots length and the rate of germination in seeds, treated with folate
analogues before planting, differed with the type of folate analogue and the concentration
used.
The fresh weight and the content of protein, DNA and RNA of callus at 15 and 30
days, were inhibited by the folate analogues.
The decrease in folate content and specific activity of dihydrofolate reductase of
callus, grown on media containing inhibitors, was similar to the decrease in fresh weight,
protein, DNA and RNA contents. Dihydrofolate reductase activity was not effected by
the addition of sulfanilamide either to the In vivo or In vitro system.

The present investigation indicated the presence and properties of cytidine
deaminase (EC.3.5.4.5) activity in the extract of the free living ciliata Tetrahymena
pyriformis.
The optimum conditions for the enzyme activity were determined.Maximum
enzyme activity was obtained in a reaction mixture of (300)μM of Tris-HCl buffer at pH (7.4) containing (3) mM of cytidine as a substrate, (250)μg protein as a source of the
enzyme, and an incubation period of (12) min. at (35)°C.
Under the optimum conditions,the specific activity was found to be(32.24±0.91)μM
of cytidine deaminated per min per mg protein in the supernatant of Tetrahymena
pyriformis extracts.The Results also indicated that cytidine monophosphate (CMP) might
be used as alternative substrate for the enzyme under investigation .

Isolated ileal strips of mouse were included in this study to investigate the effect of
alcoholic extract of colocynth pulp Citrullus colocynthis and its seeds on their smooth muscles. The results showed that ileal smooth muscles of mouse exhibits regular
autorhythmic contractions. The strength and maintenance of normal contraction and that
induced by acetylcholine and potassium chloride depended mainly on the extracellular
calcium, Also addition of calcium chloride to acetylcholine and potassium chloride
induced contraction in solution free from calcium led to augmentation and continuation
of tonic phase of the contraction.
Alcoholic extract of colocynth pulp inhibited normal contraction in a concentration
dependent manner, and free calcium solution completely closed muscle response to
alcoholic extract. It was also observed that this extract gradually inhibited acetylcholine
induced contraction.
Different concentrations of the alcoholic extract of colocynth seeds had different
effects on ileal smooth muscles. The concentrations (0.05-1) mg / ml activated normal
contraction, whereas, high concentrations (2-4) mg / ml inhibited it.

This study investigates the effects of root exudates of corn (Zea mays L.),
sorghum (Sorghum bicolor subspecies tichnicum), sunflower (Helianthus annuus L.)
,Tomato (Lycoperscum esculentum L.), pepper (Capsicum frutescens L.),and okra
(Hibiscus esculentus L.) on the growth of each others when they are planted in the same
field at the same time by applying the stair step technique to study the interference among
these plants species.
The results of the study revealed that the root exudates of the plants in question can
be classified into three distinct groups: the first group reacted positively with the root
exudates of the other plants; In other words their growth has been stimulated by the root
exudates of other plants.
It has been noticed that the growth of tomato and sorghum plants has been
stimulated significantly by the root exudates of okra plant. Also, the growth of both
sorghum and pepper plant has been stimulated by the root exudates of sunflower and has
reached 34 % for the sorghum plant.
The second group reacted negatively with the root exudates of other plants. In other
words the growth of this group has been inhibited by the effects of root exudations. The
growth of pepper plant has been significantly reduced by the root exudates of okra and
sorghum plants, the reduction rate reached (29%, 37%) respectively. The growth of
tomato and okra plants has been reduced by the root exudates of sunflower plant and the
reduction has been 43% in dry weight of tomato plant, but it has not been significant in
okra’s plant.
The third group has been affected neither positively nor negatively by root exudates
of other plant, such as the growth of pepper plant that had not been affected by root
exudates of sunflower.

Isolated ileal strips of mouse were included in this study to investigate the effect of
different concentrations of Pyrimethamine on the contraction of the smooth muscles. The
results showed that ileal smooth muscles of mouse exhibits regular autorhythmic
contractions. This normal contraction mainly depended on the extracellular calcium in
their tension and maintenance .
Pyrimethamine caused a concentration dependent relaxation, and a high
concentration (8-14 mM) of it rapidly caused relaxation for a normal contraction. Free calcium solution reduced smooth muscles response to pyrimethamine, The low and
moderate concentrations (0.2 and 8) mM led to a partial inhibition of the rhythmic
contractions and inhibition of tonic phase of acetylcholine induced contraction.

The present study aimed at investigating the inhibitory effect of cardamom
(Elelteria cardamomum) towards the genotoxic effects of two drugs; Cyclophsphamide
(CP) and Methotrexate (MTX) in mice. Four cytogenetic parameters were employed to
achieve the aim. Those were mitotic index of bone marrow and germ cells, chromosomal
aberrations, micronuclei test and sperm head abnormality assay.
Cardamomum fruits were subjected to extraction with two solvents, water and
ethanol. From each extracts, four gradually increased doses were evaluated for their
toxicity. Doses from plant extracts with no toxic effect was chosen for drug-plant extract
interaction was employed in (Balb/c mice). Genotoxicity of CP and MTX were also
tested in mice.
Cadamomum extracts showed no toxic effects at doses up to 50 mg/kg which were
pictured by cell divisions of bone marrow and germ cells. While both drugs CP and MTX
exert genotoxic effects at 100 and 3.25 mg/kg respectively. These plant and drug doses
were applied for plant drug, and drug–plant interaction studies.
The result revealed that the aqueous and alcoholic extracts of cardamomum exhibit
a good protective activity. This was depicted by increasing the mitotic activity and
reducing the chromosomal aberration, micronucleus, and sperm head abnormalityfrequencies.
The alcoholic extract was more potent in protection activity compared to
equalize extract specially when it was proceeding the drug treatment.

The research was concerned with the isolation of urease form local Vica faba
using different biochemical techniques.
It was shown that gel filtration chromatography of the proteinous precipitate
which was produced by acetone, heat treatment and acid precipitation gave three
proteinous peaks. The first and the second peaks possessed a variable activity of urease
where maximum specific activity was obtained in the second peak which showed (40.1)
folds of purification.
Furthermore, the comparative molecular weight of the partially purified urease
using both sodium dodecyl sulphate-polyacrylamide gel electrophoresis and gel filtration
chromatography techniques was found to be (460000 ± 5000)and (480000 ± 8000) dalton
respectively .
The optimum conditions of urease was obtained using (50 mM) sodium phosphate
buffer at pH (8.3) with incubation temperature (50) C, incubation time (30) minutes and
(5 mM) of urea as a substrate. Using Lineweaver-Burk plot, it was found that Vmax and
Km had the values of (0.8) unit/ml and (6.06 mM) respectively.
The results were also indicated that the activity of the enzyme decreased gradually
to (70%) and (59%) when the enzyme was stored for (30) days at (4)Cْ and room
temperature respectively. The activity of the enzyme was inhibited by CuCl2 or methyl
urea where the inhibition is competitive .
Finally, the results predicted that there was no significant differences between the
activity of urease extracted from local Vicia faba and the urease used in the standard kit
for the determination of urea in blood for normal and patients.

Widespread occurrence of stromatolitic horizons is documented in the middle part
of Mulussa Formation, of the Gaara depression western of Iraq. These stromatolitic
horizons are associated with intertidal channels, fenestral (birdseye) porosity, trace fossils
of Rhizocorallium, as well as chert nodules with varnished evaporites. The main petrographics constituent appeared to be calcite with radial pattern
enclosed between dark algal walls, later replacement by dolomite and silica is common.
Based on the sedimentary attributes and characteristic features of these stromatolitic
horizons and their comparison with recent and ancient analogous, an intertidal-lagoonal
environment is inferred during their deposition.

This work aims to find the methods that help Cryptanalysis to break one of secret
key system, that is stream cipher by used artificial neural network as plain text attack
instead of using the old known methods, such as matrix and massy algorithm to break
stream cipher.
C++ Language Version (3.0) was used in this study on computer type (Pentium
300 MHZ)

A study of Gamma-ray effects on the optical absorption of glass for a range of
wavelengths ( 300-900 ) nm with doses varied between (0.331-2.65) Mrad. The
effect of annealing treatment on the optical absorption within the range of (50-400)°C
has been also studied, then the calibration line for measuring the irradiation dose for
Gamma-ray on the glass was established. Two models were used to measure the
activation energy at fixed annealing time (0.25) hr.

In this work firstly, a parallel plat capacitor with thin film of Poly Mathyl Metha
Acrylate (PMMA) have been fabricated for different thickness using a spinner technique.
Secondly, the effect of gamma ray radiation on electrical properties of these capacitor
have been studied. Electrical characteristic of un-exposured and exposured capacitor to
gamma ray for different periods times have been studied. The results show that the breakdown voltage, in general, is decreased an exponentially when the capacitor
exposured to a certain time of radiation. while it’s reveal an increasing breakdown
voltage with the increasing in irradiation period time. This work is also include the study
of the effect of radiation on electrical conductivity. The results show that there is an
exponentially decreasing in electrical conductivity as increasing exposured time for
gamma ray. Finally, the effect of gamma radiation on the value of capacitance for the
fabricated capacitor, have been studied. The results show that there is an increasing in it’s
value as increasing in exposured time.