Rafidain journal of science

The anodic oxidation and cathodic reduction of coenzyme Qo (CoQ0) were studied
using cyclic voltammetry (CV) at graphite electrode (C) in Britton -Robinson buffer
(B.R.B) of pH ( 2-9). The result shows that the charge transfer processes were reversible over all pH range. The method is used to determine CoQ0 in concentration range
( 8.7 ×10-5 – 1.9 × 10-6
) M at pH = 2 with a reduction potential of – 0.325 V and – 0.235
V for reduction and oxidation processes versus Ag / AgCl sat. KCl reference electrode.

Adenosine deaminase specific activity was studied in serum and erythrocyte
haemolysate for patients with leukemia.
The results show an increase with a significant difference of activity enzyme in
serum and erythrocyte for patients compared with normal individuals of the same age and
sex. The results obtained showed adenosine deaminase specific activity by unite of nmole
of adenosine deaminated/min/mg protein in serum and erythrocyte haemolysate of
patients were (35.42±5.64) and (94.48±5.99) respectively, while the mean value of
activity in normal individuals were (13.68±1.72) and (60.56±6.55) in serum and
erythrocyte haemolysate respectively.
The analysis also indicated that factors of sex, age and type of disease Acute
Lymphocytic Leukemia or Chronic Myelocytic Leukemia have no effect on the activity
of enzyme in patient’s serum and erythrocytic haemolysate. Moreover, higher
concentration of total protein the decline in calcium level is found in the serum of the
patients, while no significant deference of alkaline phosphates and hemoglobin in
leukemia and normal individuals
Finally, the statistical results has been shown that positive correlation between
activity enzyme and total protein (r=0.369;p<0.05) and negatively with calcium (r=0.505;
p=0.0001) in patient’s serum.

This work involves estimation of parathyroid hormone (PTH) level in renal failure
patients undergoing hemodialysis in Ninavah governorate. Sixty four samples collected
from those patients were (35 male and 29 female) of age (15-66 years), were compared
with fourty five samples as control group (22 male and 23 female) of age (15-63 years).
The studied biochemical parameters of blood sera, included, PTH, total calcium
(T.Ca), inorganic phosphorous (PO43-), albumin (Alb) and alkaline phosphatase activity
(Alp). The results showed high significant levels of PTH, PO43- and Alp. In the same
time the study demonstrated that lower significant levels of T.Ca and Alb compared with
control group (p < 0.05). The study of effect of the independent variables with respect to
patients group on the level of the measured biochemical parameters were indicated that
sex and number of hemodialysis per week had a significant effect on the level of PTH,
while the period of treatment showed a significant effect on Alp levels. In the same time
the age showed a significant effect on Alb level (p < 0.05).

The research, involved Biochemical study of leptin, Insulin and their relationship to
some minerals in diabetic patients (Type I and Type II ) compared with normals .
The results demonstrated that the normal mean of Leptin hormone in human blood
serum (19.5 ± 5.6 ng / ml), and body weight affected Leptin concentration, while the
results demonstrated a significant decrease in the level of Leptin and Insulin levels in
serum of type I diabetic Patients in comparison with control and a significant difference
in the mean levels of Leptin and Insulin between type I and II .
The results also demonstrated a significant decrease in the means of Potassium and
Magnesium concentration in patients type I compared with control group, while non
significant decrease of Sodium and Zinc in diabetic Patients type I and II compared with
control .
Correlation coefficients between Leptin, Insulin hormone and some minerals of
diabetic patients showed that Leptin hormone has a positive linear relationship with
Magnesium and Zinc in control and diabetic patients type I and II, while Insulin hormone
has a positive linear relationship with Magnesium and Zinc in control and diabetic
patients type II, and showed a positive linear relationship with Magnesium in diabetic
patients type I.
Also the results demonstrated a positive linear relationship between Leptin, Insulin
hormone and Potassium in control group .

The research included a biochemical study of patients with duodenal ulcer (DU).
Histological biopsies were taken for the pathological and bacteriological tests,
investigating H pylori infection. Blood (76 samples) and gastric juice (61 samples) were
taken from patients with DU. The measured parameters in sera included :Vitamin C,
Vitamin E, Vitamin A, Pepsinogen, total protein, albumin, chloride, iron, hemoglobin
and blood groups, whereas in gastric juice included: vitamin C, Pepsinogen, chloride,
total protein and urea.
The results showed a significant decrease in vitamin C, vitamin E, vitamin A, iron
and hemoglobin concentration in the serum of patients with DU, in comparison with
control group, for both sexes. Pepsinogen was also found to be increased significantly in
both blood and gastric juice of patients with DU, for both sexes. A significant increase in
blood and gastric juice chloride concentration was found for patients with DU for both
sexes. The results had also shown no significant difference in blood total protein
concentration for patients with DU whereas a significant decrease in gastric juice protein
concentration was noticed for patients with DU for both sexes. It had pointed that serum
albumin concentration was within the normal range for patients with DU for both sexes.
Finally the results had demonstrated a significant decrease in gastric juice urea and
vitamin C concentration for patients with DU in comparison with control group for both
sexes. However, the results had also confirmed that persons with blood group O+ are
more liable to have duodenal ulcer .

The research was an attempt to isolate and study the active protienous compounds
from the cold aqueous extract of Coriandrum sativum L. using different biochemical
techniques.Two compounds I and II were isolated using gel filtration chromatography of
the precipitate produced by cold acetone precipitation.The comparative molecular weights
of I and II were found to be in the range of 70800 and 4000 dalton respectively.
The work also included the study of the aqueous extract and the proteinous
compounds on certain blood constituents and liver glycogen in normal and
alloxan-induced diabetic mice. In normal mice, blood glucose level was lowered by
concentrated aqueous extract and low molecular weight proteinous compound II to 54.4%
and 50.4%. The aqueous extract and compound II also reduced the level of total cholesterol
to 27.8% and 30.8%, whereas only the aqueous extract produced an increase in glycogen
content in the liver of normal mice. In diabetic mice, the aqueous extract and compounds I
and II lowered glucose level to 65.8%, 74.3% and 49.3% and also reduced the levels of
total cholesterol and total lipids significantly. In addition, compound I showed a significant
increase in glycogen content in liver of diabetic mice.