Rafidain journal of science

A sample of 24 spontaneous mutants resistant to the antifungal itraconazole were
isolated in the brown strain A76 (18 mutants) and the white strain AZG131 (6 mutants)
of the fungus Aspergillus amstelodami . Dominance tests, in heterokaryons, have shown
that all mutants are recessive to their respective wild type alleles. Complementation tests,
also in heterokaryons, among the mutants indicated that all mutantions belong to a single
gene given the gene symbol itzA as it was the first gene of its kind to be identified in this
fungus. There are nine (1-IX) linkage groups so far recognized in this fungus and
haploidization analyses of diploids between one of the mutants and suitable master stains
put the gene itzA outside groups I-VII, but its relationship to groups VIII and IX was not
determined as no master strains carrying markers on these two groups were available at
the time. Also, the biochemical function of itzA in relation to the three genes cyp51A,
cyp51B and MDR, recognized to confer resistance to itraconazole in Aspergillus could
not be determined.

Twenty bacterial isolates were collected from human diarrheal samples and then
identified. Seven out of twenty isolates were E. coli and three P. mirabilis . These isolates
were checked for their resistance to six antibiotics and two heavy metals ( HgCl2 and
Cdcl2). The bacterial isolates showed variation in their resistance to antibiotics and heavy
metals.and only one E. coli but all P. mirabilis isolates showed high level of resistance.
Transposition induction of genes in the two chosen bacterial isolates was carried out..
Our results suggested that in E. coli isolate, neomycin resistance gene was induced to
jump and insert itself into chloramphenicol and tetracyclin resistance genes causing them
to mutate .in percents reaching 80% and 8% respectively. On the other hand, the
streptomycin resistance gene transposition appear to be specific for generating mutations
in the heavy metals resistance genes at rates 48% and 100 % respectively. In addition,
transposition induction of the above genes in the chosen P. mirbilis isolate seemed to
cause mutations in ampicilin, chloramphenicol and tetracyclin resistance genes ranging
between 2 and 4 % .

In order to determine the effects of toxoplasmosis on the lipid peroxidation and the
glutathione defense system against peroxides. Forty pregnant women with toxoplasmosis
(acute and chronic) and twenty healthy pregnant women (control group) with age range
of 18-45 years living in Mosul city were selected. Serum malondialdehyde (MDA),
reduced form of glutathione (GSH) and gluthathione peroxidase activity (GSH-PX) were
measured. The results showed a marked increased in MDA level (as an index of lipid
peroxidation) in patients with toxoplasmosis in comparison with the control group. On
the other hand, patients with toxoplasmosis had decreased GSH and increased GSH-PX
level when compared with the control subjects.

The use of natural mineral inert dusts is considered one of the methods for
protection of stored grains from insect pests infestation.
Four local rocky dusts were evaluated against four storage insects. Ninivite dust
proved to be the most effective of the tested dusts and gave LC50 of 0.12, 0.14, 0.06 and
0.08% for each of T. confusum, T. granarium, O. surinamensis and R. dominica while
bentonite dust showed the lowest effect and gave LC50 of 0.74, 0.85, 0.13 and 0.20 for
the insect species, respectively. The tested dusts arranged in the follwing order: ninivite >
kaolinite> montorillonite> bentonite.
The effect of ninivite dust to O. surinamensis was 2.41, 2.0 and 1.4 times more than
T.granarium , T. confusum , and R.dominica , respectively. The use of ninivite dust to
prevent insect infestation in stored grains is a successful alternative to insecticides.

An experiment was conducted to investigate the effect of aflatoxin at a
concentration 2,5 mg/kg diet alone or with different levels of Ninivite 0.5, 1, 1.5, 2 and
2.5% on total, differential and absolute leukocyte counts in broiler chicks. The addition
of aflatoxin (AF) in broilers diet at a concentration of 2.5 mg/kg did not significantly
alter leukocyte counts when compared with the control group. The addition of Ninivite at
all doses to the AF contaminated diet were resulted in a significant increase in the total
circulating leukocytes when compared with the control group.
Differential leukocyte counts revealed a significant increase in the percentages of
both monocytes and heterophils, and a reduction in lymphocytes when Ninivite was
added at all doses to AF contaminated diet. The differential leucocyte counts of the
remaining cells (Basophils and Eosinophils) show no significant changes when compared
with AF and control group. The heterophils lymphocytes (H/L) ratio was significantly
increased with each increase in the level of Ninivite addition to AF contaminated diet
when compared with AF and control group .The picture of the absolute and differential
leukocyte counts were identical except that, the lymphocytes were significantly reduced
when Ninivite was added to AF contaminated diet at concentrations more than 1.5%
when compared with AF and control group.

The scolicidal effects of crude ethanolic extract and crude alkaloids of
Prosopis farcta leaves on protoscolices of Echinococcus granulosus were appraised in
vitro, in comparison with mebendazole (the drug of choice in the treatment of
echinococcosis) at 37C0 and 4C0 and at different time intervals.
Both extracts exerted higher effect at 37C0, and the crude alkaloids were more
effective than ethanolic extract as it gave a scolicidal effect especially at the lower
concentrations used (62.5, 31.2 mg/ml) within shorter periods (3 days and 13 days at
37C0 and 4C0 respectively)