Rafidain journal of science

1

Square Wave Voltammetric Method for the Determination of Substituted Oxadiazol in Aqueous Solution

Pages: 1-6 English

Abstract

A rapid, sensitive and accurate method for the determination of substituted oxadiazol
(I&II) has been developed based on the reduction of electroactive group (-C=S) at
hanging mercury electrode by using square wave voltammetric technique. The reduction
peaks obtained at –0.028 and –0.172 V Vs Ag/AgC1 fir compounds I & II respectively at
pH 2 in Britton – Robinson buffer are recommended for the trace determination of these
compounds. Regression analysis on standard curve indicated a linear relationship
between peak current and concentration over the rang 1.2 × 10-6–2.5 × 10-5 M with a
correlation coefficient of more than 0.98.

2

Spectrophotometric Determination of Sulphamethazine by the Diazotisation-Coupling Method with m-Aminophenol as the Coupling Agent-Application to Pharamaceutical

I’tmad A. Taha

Pages: 7-14 English

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Abstract

spectrophotometric method for the trace determination of sulphamethazine has
been worked out. It is based on the reaction of sulphamethazine with sodium nitrite in
acidic medium to form the corresponding diazonium salt. After removal of excess nitrite
with sulphamic acid, the diazotised sulphamethazine is coupled with m-aminophenol fo
form an intensely-yellow coloured, water-soluble and stable azo dye which shows
maximum absorption at 457 nm. The plot of absorbance versus determinand
concentration shows that Beer’s law is obeyed over the concentration range 5-150 μg
sulphamethazine/25 ml final volume, i.e., 0.2-6 ppm with a molar absorptivity of 4.8X104
l.mol-1.cm-1. The method has been applied to the assay of the drug in a tablet.

3

Synthesis of 1,2,4-Oxadiazolidine and Isoxazolidine Derivatives from Addition of Isocyanates and Alkenes to Nitrones

Mohammad S. Al-Ajely, Huda A. Basheer, Namer S. Ezzat

Pages: 15-21 English

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Abstract

Some new heterocyclic compounds of 1,2,4-oxadiazolidine-5-one derivatives
[3a-m] were synthesized from the reaction of nitrone compounds with some isocyanates
of amino acid esters in good yields.
The reaction of nitrone compounds with cyclooctene and tetrahydrophthalic
anhydride gave oxazolidine derivatives [4a-d]. The synthesized compounds were formed
via 1,3-cycloaddition reaction of isocyanates and alkenes to nitrones. The I.R. spectral
data of the synthesized compounds were also discussed.

Abstract

يتضمن البحث تحضير عدد من مشتقات 1،2،4 -اوكسادايازولين- 5-اون [3a-m] من تفاعل
مركبات النايترون مع بعض ايزوسيانات استرات الاحماض الامينية . كما ان تفاعل مركبات النايترون
4] . حضرت a-e] مع سايكلواوكتين ورباعي هيدروانهيدريد الفثاليك اعطى معوضات الايزوكسازوليدين
المركبات اعلاه من خلال الاضافة الحلقية 1،3 للايزوسيانات والالكينات الى النايترونات . تم مناقشة
نتائج المركبات المحضرة من خلال طيف الاشعة تحت الحمراء .

4

The Electrical Conductivity of a Mixture of Both Symmetrical and Unsymmetrical Complexes in Methanol At 25 °C

Banan A. Akrawi

Pages: 22-34 English

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5

Some Biochemical Studies on Serum of Duodenal Ulcer Patients Infected with Helicobacter pylori in Ninevah Governorate

Layla A. Mustafa, Tareq Y. Ahmad, Bahyj Y. Al-Khalil

Pages: 35-44 English

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Abstract

The research was concerned with biochemical studies in serum of duodenal ulcer
(DU) patients. The present study included (152) patients (105 males and 47 females),
their ages ranged between (15-56) years, attending endoscopy unit of Al-Razzi General
Teaching Hospital in Ninevah Governorate.
Blood samples from (152) DU patients, were collected for some biochemical
parameters including alkaline phosphatase (ALP), glutamate pyruvate transaminase
(GPT), urea, total protein, Immunoglobulins (Igs), and haemoglubin (Hb).
The results predicted that GPT, urea, total protein, IgG and complement C4
statistically showed no significant difference with H. pylori infection, while ALP, IgM,
IgA, complement C3 and Hb showed significant differences

6

Synthesis of New Derivatives for Substituted Malonamic Acids

Mohammad A. Sheat

Pages: 45-53 English

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Abstract

A number of substituted malonamic acids derivatives were prepared by
nucleophilic substitution reactions of substituted malonyl chlorides with phenyl
carbamate followed by reaction of the products with water, alcohol and amine. The
structures of the synthesized compounds were elucidated by physical and spectroscopic
methods.

Abstract

حضرت عدد من مشتقات حوامض المالوناميك المعوضة من خلال تفاعلات التعويض النيوكليوفيلية
لكلوريدات المالونيل المعوضة مع كاربامات الفنيل ومفاعلة النواتج مع الماء والكحول والامين، وتم اثبات
الصيغ التركيبية لهذه المركبات باستخدام الطرائق الفيزياوية والطيفية.

7

Nutritional Rickets in Northern Iraqi Children – Response to Vitamin D

Samir B. Al-Mukhtar, Akram J. Ahmad

Pages: 54-59 English

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Abstract

The biochemical bone profile (BBP) which include serum calcium, phosphorus,
alkaline phosphatase (ALP) and albumin was studied on twenty seven rachitic children
aged 6-60 months who attended the outpatient department in Al-Khansa maternity and
children's Hospital in Mosul during the period from July 2000 to April 2001 and were
followed up 6-8 weeks after treatment by oral massive single dose of vitamin D . The
radiological and biochemical characteristics of cases ware ascertained before and after
therapy .The biochemical changes ware analyzed by student (t) test.
There was a significant increase in the level of serum calcium(p<0.006),
phosphorus, and albumin (P<0.0001), with a significant decrease in the ALP activity after
vitamin D therapy with no risk of hypercalcaemia (P< 0.0001).
In conclusion, ALP is the main test in the follow up of rachitic children ,also oral
massive single dose of vitamin D is effective in reversal of BBP to normal levels with no
risk of hypercalcaemia
Key words: Rickets, Calcium, Phosphorus, Albumin, Alkaline phosphatase, Biochemical
Bone Profile, Vitamin D.

Abstract

الواجهة الكيماوية الحيوية للعظام والمقصود بها فحوصات الكيمياء الحيوية المكونة من الكالسيوم
و الفسفور اللاعضوي و فعالية إنزيم الفوسفاتيز القاعدي و الزلال (الألبومين ) تمت دراستها على سبعة
60 شهرا و الذين كانوا يراجعون العيادة الخارجية في - و عشرين طفلا مصابون بالكساح من عمر 6
8 أسابيع من العلاج بجرعة - مستشفى الخنساء للولادة و الأطفال في الموصل و تمت متابعتهم مدة 6
فموية واحدة من فيتامين د . أجريت لهم فحوصات الأشعة و الواجهة الكيماوية الحيوية قبل و بعد العلاج.
و كانت هناك زيادة معتدة t تمت دراسة مقارنة التغيرات الكيمياوية الحيوية بواسطة اختبار
إحصائيا في مستوى الكالسيوم ( ب < 0.006 ) والفسفور و الألبومين (ب< 0.0001 ) مع انخفاض معتد
إحصائيا في فعالية إنزيم الفوسفاتيز القاعدي (ب< 0.0001 ) في كافة الحالات بعد العلاج .لم تكن
هنالك خطورة فرط الكالسيوم بعد العلاج بفيتامين د.

8

Diazotised p-Nitroaniline Reagent for the Determination of Trace Amounts of Salbutamol Sulphate in Aqueous Solution– Application to Pharmaceutical Preparations

Nabeel S. Othman, Inaam A. Hamdon

Pages: 60-67 English

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Abstract

A simple, rapid and sensitive spectrophotometric method for the determination of
micro amounts of salbutamol sulphate in aqueous solution has been developed. The
method is based on the coupling of salbutamol sulphate with diazotised p-nitroaniline in
basic medium to form a coloured mono azo dye which is stable, water- soluble and
exhibits maximum absorption at 488 nm. Beer’s law is obeyed over the range 10–120 μg
of salbutamol sulphate in a final volume of 25 ml, i.e., 0.4–4.8 ppm with a molar
absorptivity of 3.13×104 l.mol.-1cm-1, a relative error of +1.80 to –3.40% and a relative
standard deviation of +5.70 to +1.65%, depending on the concentration level. The
proposed method does not require temperature control or solvent extraction and the
method has been successfully applied to the determination of salbutamol sulphate in
different types of pharmaceutical preparations, tablets and syrup.

9

Effect of Substituents Type on the Adsorption of Aromatic Carboxylic Acids and their Relation to Concentration, Temperature and pH

Emad A. Al-Hyali, Omar M. Ramadhan, Safwan A. Al-Dobone

Pages: 68-79 English

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Abstract

The study involved investigation of the adsorption of aromatic carboxylic acids
carrying various substituents such as OH, NH2 and SH. The effects of concentration,
temperature and pH were induced. The results indicated that increasing the temperature
from 15 to 30 oC reduces the adsorption ratio, while dilution of carboxylic acid solution
to a little value showed high adsorptivity. This point is of a valuable interest because
most of carboxylic acids as pollutants present in a minute amounts.
Effect of pH showed that the natural pH is the best condition for the removal
which is arreptable for the treatment of industrial water. According to the literature we
assumed that after one hour of extraction the system will be in equilibrium. Therefore,
from this point we determined the thermodynamic functions for each system under
investigation. The study revealed that the hydrogen bond present in the system and other
attraction forces with the resonance have great effect on the adsoption

10

Study of Charge Transfer Complexes of salicylidene aliphatic amines with Eu(fod)3 in cyclohexane by U.V. spectroscopy

Layla M.N. Saleem, Sadallah T. Sulaiman, Isam J. Al-Nuri

Pages: 80-87

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Abstract

Salicylidene aliphatic amines in cyclohexane form 1:1 charge transfer complexes on addition of Eu(fod)3, indicated by the observation of a new band in their U.V. spectra located at longer wavelength (= 400 nm) and with low extinction coefficient. The coulorless solutions of Schiff-bases were changed to yellow on complexation with the shift reagent Eu(fod)3.
Equilibrium Constant values (K) for the complexes were obtained by applying Benesi-Hildebrand equation. The factores which affected the values of of K like the charge on N, O & C (ChN, ChO, ChC), steric energy, O-N distance, the dihedral angles (dh1 and dh2) were established computationaly and were inserted into an equation of multiple linear regression as independent variables with K as dependet variable:

K= -1288.806-1653.722ChN-40.197ChO-914.560ChC+507.207(O-N dist.)

From the equation it is seen that the most effective factor is ChN which means that increasing ChN causes more availability of the lone pair of electrons on nitrogen which facilitate the interaction with Eu(fod)3 and thus leading to higher K-value.

Abstract

تكون مركبات السالسلدين أمين الالفاتية في مذيب السايكلو هكسان معقدات انتقال الشحنة من نوع 1:1 عند اضافة كاشف الازاحة 3(Eu(fod، ويستدل على ذلك من ظهور حزمة جديدة في اطياف الاشعة فوق البنفسجية عند طول موجي بحدود 400 nmوبمعامل انطفائية واطئ، كما لوحظ ان المحاليل عديمة اللون لقواعد شيف قد تغيرت الى محاليل صفراء عند تكون هذه المعقدات.
وتم ايجاد قيم ثوابت الاتزان (K) لهذه المعقدات باستخدام معادلة بنسي هلدبراند، ومن ثم دراسة تاثير بعض العوامل على K مثل: الشحنة على الكاربون، الاوكسجين، النتروجين (ChC, ChO, ChN)، طاقة الاعاقة، المسافة O-N والزاويا بين المستويين dh1 و.dh2 وادخلت هذه العوامل في معادلة الانحدار الخطي كمتغيرات مستقلة بينما اعتبرت K متغيرا غير مستقل:
K =- 1288.806-1653.722ChN-40.197ChO-914.560ChC+507.207(O-N dist.)
من هذه المعادلة يتضح ان اكثر العوامل تاثيرا هو ChN وهذا يعني ان زيادة ChN تجعل زوج الالكترونات على النتروجين اكثر توفرا مما يسرع في عملية الاتحاد مع كاشف الازاحة 3(Eu(fod وبذلك تزداد قيم K.

11

Application of γ-Radiolysed Natural Silica on the Fractionation of Virgin Olive Oil

Akram A. Mohammed

Pages: 88-94 English

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Abstract

The study of γ-radiolysed natural Iraqi silica (Ninivite) as obtained from the main
rocks indicated that as we increase the irradiation time, the ratio of amorphous silica to
the crystalline one is increased. A significant effect is recognized when irradiation time
approximately two hours. The radiation causes cleavage of the polymeric structure of the
silica and produce monomeric silica of amorphous type. The application of the irradiated
silica to the separation of virgin olive oil indicates a large difference between one fraction
and another as shown by the amount of fractions and I.R. measurements. I.R.
measurements indicate that olive oil contains fatty acids, fatty acid esters, phenols,
alcohol and some other minor components.

Abstract

يهدف البحث الى دراسة التفكك الاشعاعي للسليكا الطبيعية كما وردت من خاماتها الام.
واوضحت عملية التشعيع ان السليكا البلورية ذات الوزن الجزيئي العالي قد تفككت وازداد تفككها بزيادة
زمن التشعيع حتى اصبحت اعلى ما يمكن عندما كان زمن التشعيع ساعتان. ويعتقد ان السليكا قد تفككت
وانتجت وحدات مونومرية متكافئة تقريبا تعاني من خلل بلوري اطلق عليها السليكا غير البلورية وكما
توضح ذلك في عملية الفصل بوساطة هيدروكسيد الصوديوم. ان تطبيق عملية الفصل لزيت الزيتون
العذري على هذه السليكا المشععة اظهر فروقات كبيرة ما بين الاجزاء المفصولة واستقطابيتها اذا ما
قورنت بتلك التي فصلت من السليكا غير المشععة. وقد اوضح طيف الاشعة تحت الحمراء للاجزاء
المفصولة، ان زيت الزيتون العذري خليط من الاحماض الدهنية واستراتها وبعض الفينولات اضافة الى
وجود الكحولات هذا الى جانب وجود بعض المكونات الجزئية .

12

Polarographic Measurement of Lactate Dehydrogenase Activity in Human Serum “Clinical Application”

Sadallah T. Sulaiman, Talal S. Al-Najafi, Hisham S. Hamdon

Pages: 95-99 English

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Abstract

The paper reports a sensitive and rapid method for the measurement of Lactate
Dehydrogenase activity (EC 1.1.1.27) in human serum. The method is based on the
polarographic measurement in the reduction of polarographic peak of NAD+ via the
enzymatic reaction of LDH with lactate and NAD+.
The method was successfully applied to measure LDH activity in serum of normal and
diseased individuals after the removal of albumin by zinc acetate method.

Abstract

تم توضيح طريقة سريعة وحساسة لقياس فعالية انزيم لاكتيت ديهيدروجنيز في مصل الدم. حيث
خلال التفاعل للاكتيت NAD+ تعتمد الطريقة على قياس الانخفاض في الموجة البولاروغرافية ل
في مصل الدم بعد ازالة الالبومين باستخدام طريقة خلات NAD+ ديهيدروجنيز مع اللاكتيت وبوجود
في مصل الدم للافراد الطبيعيين LDH الخارصين. وقد تم تطبيق هذه الطريقة بنجاح لقياس فعالية انزيم
والمرضى

13

Synthesis of some Azo Compounds Derived from Substituted 4`-Aminosulfonanilides

Mohammad A. Al-Iraqi, Kawther H. Al-Naimi, Haiffaa Y. Hussain

Pages: 100-106

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Abstract

The synthesis of azo compounds (9-11) has been achieved by reaction of diazonium
salts of substituted 4`-aminosulfonanilides with some aromatic amines or phenols
4-Nitroaniline (1) is served as a synthon for 2,6-dibromo-4-nitroaniline (6). The
compounds (1 and 6) undergo sulfonylation with methane- and 4-toluene sulfonyl chloride
to give the corresponding sulfonanilides (2, 3, 7). The nitro group in these compounds is
reduced to amino group to produce compounds (4, 5, 8). These compounds are diazotised
to the corresponding diazonium salts, which are coupled with different aromatic amines or
phenols to produce the azo compounds (9-11). The structure of the synthesized compounds
has been confirmed on the basis of physical and spectral measurements.

14

Coordination Compounds of Tin, Selenium and Bismuth. Complexes of Tin(IV), Selenium(IV) and Bismuth(III) with Schiff Bases

Amer A. Taqa

Pages: 107-117 English

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Abstract

Tin, selenium and bismuth metal complexes containing Schiff bases ligands derived
from ortho-benzaldehyde, dimethyl ketone, diethyl ketone and di(o-aminophenyl)
disulphide have been prepared from the direct reaction of Schiff base and metals in 1:2
molar ratio to give:
Sn(SC6H4N=CHC6H4OCH3-o)4, Sn(SC6H4N=C(CH3)2)4, Sn(SC6H4N=C(CH2CH3)2)4,
Bi(SC6H4N=CHC6H4OCH3-o)3, Bi(SC6H4N=C(CH3)2)3, SC6H4N=C(CH2CH3)2)3,
Se(SC6H4N=CHC6H4O CH3-o)4, Se(SC6H4N=C(CH3) 2)4 and Se(SC6H4N=C(CH2CH3)2)4.
The reaction of the above metals with a mixture of iodine and Schiff bases give in
(1:1:3) molar ratio the compounds SnI(SC6H4N=CHC6H4OCH3-o)3,
SnI(SC6H4N=C(CH3)2)3, SnI(SC6H4N=C(CH2CH3)2)3, BiI(SC6H4N=CH-C6H4OCH3-o)2,
BiI(SC6H4N=C(CH3)2)2, BiI(SC6H4N=C(CH2CH3)2)2, SeI(SC6H4N=CHC6H4 OCH3-o)3,
SeI(SC6H4N=C(CH3)2)3 and SeI(SC6H4N=C(CH2CH3 )2)3. The compounds were
characterized by microanalysis, infrared, UV/Visible spectroscopy and conductivity
measurement.

15

Aging Effect in Different Aqueous Media on Thermal Stability of Epoxy Resin

Akram A. Mohamed, Qais M. Abd Al-Hameed

Pages: 118-125 English

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16

Approaches to the Synthesis of Tetracyclic Quinone System Related to Anthracyclinone

Badie A. Ahmed Abdul, Hakeem T. Mahmood

Pages: 126-132 English

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Abstract

Synthesis of the tetracyclic quinone compound (10) is described. The synthetic
route adopted involves: preparation of the precursor 2-(2’-methoxy carbonyl benzoyl) –
1,4-benzoquinone (7), Diels-Alder reaction of the later with cyclopentadiene, [1,5]-aroyl
migration in the resulting adduct (8), and finally ring closure in (9).

Abstract

تم تحضير مركب الكوينون ( 10 ) ذات الحلقات الاربع باعتماد مسلك تشييدي اشتمل على تحضير
2،-ميثوكسي كاربونيل بنزويل)- 4،1 -بنزوكوينون ( 7) كمادة وسطية، تفاعل ديلز-ألدر بين )- المركب 2
المركب الاخير والبنتادايين الحلقي لتكوين المركب ( 8)، هجرة مجموعة الاروايل من نوع [ 5,1 ] لتكوين
.( المركب ( 9) واخيرا غلق حلقة الكوينون للحصول على الناتج النهائي

17

Synthesis and Characterization of Some Zinc(II) and Cadmium(II) Complexes with Schiff Bases

Akram A. Mohammed

Pages: 133-137

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Abstract

A number of zinc(II) and cadmium(II) complexes with two Schiff base ligands have
been prepared. The ligands derived from the condensation of furfuraldehyde with
butylamine (FBA) and hexylamine (FHA). The complexes which have the molar ratio of
1:2 (metal to ligand) had general formula [M(L)2]X2 (where M = Zn(II) or Cd(II) ; while
X = Cl, NO3 or ½ SO ). The complexes were characterised by molar conductance
measurements and the study of their infrared and ultraviolet absorption spectra, while
zinc and cadmium contents were determined by standard procedures. These studies
revealed the action of both ligands as neutral bidentate chelating ligands, formed
complexes with tetrahedral structure.

18

Synthesis and Characterization of Trinuclear Complexes and Adducts through Oxidative Addition Reaction of Thiuram Disulfide

Nabeel H. Buttrus, Amena F. Al-Omari

Pages: 138-145 English

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19

Unexpected Products in the Synthesis of Tetracyclic Anthrone Systems

Badie A. Ahmed Abdul, Hakeem T. Mahmood

Pages: 146-154 English

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20

The Electrical Conductivity of Some Alkali Nitrates in Propanol- Water Mixtures at 298K

Banan A. Akrawi Salem M., Khalil Ashur M. Dawod

Pages: 155-164 English

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21

An Application of Lee-Wheaton Conductance Equation to Mixed (Symmetrical and Asymmetrical) Electrolytes in Methanol at Two Different Temperatures

Banan A. Akrawi, Yaser O. Hameed

Pages: 165-175 English

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22

The Effect of Proteinous Compounds from Myrtus communis L. Fruit on Some Biochemical Parameters in Mice

Tareq Y. Ahmad

Pages: 176-185 English

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Abstract

Three compounds (A, B and C) were isolated by gel filtration chromatography of
the precipitate produced by acetone precipitation of the aqueous extract of Myrtus
communis L. fruits. Comparative molecular weights determination by gel filtration using
standard proteins gave a value of 74990, 6310 and 620 dalton respectively. A single
intraperitoneal injection of the isolated compounds (A, B and C) at a does of 77.5 mg/Kg
was given to normal mice and some biochemical parameters were determined. The
results of injection showed a significant decrease of serum glucose, cholesterol,
triglycerides, total lipids level and glycogen content in liver tissues for both component A
and B at different variation as compared to control group. However, compound C showed
only a significant decrease in glycogen content in liver tissues.

23

Radical Behavior Produced in -Irradiated Alanine and Phenylalanine Amino Acids Aqueous Solutions

Azhar A. Abdul-Rahman, Khalid Y. Al-Yamoor

Pages: 186-192 English

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Abstract

Radiolysis of alanine and phenylalanine in aqueous solutions containing NADH
has been carried out at different gas saturation and pH. It has been found that the
oxidation of NADH decreases by increasing pH. Also, it has been observed that the yield
of NADH oxidation in N2-saturated aqueous solutions is higher than that observed in
oxygenated irradiated solution. It is concluded that more oxidizing radicals are generated
through the deamination process as a result of the electron addition on the ammo acid.
The results also have shown that the yield of NADH oxidation is higher slightly in the
case of alanine as compared to that in the case of phenylalanine.

Abstract

تم دراسة التحلل الاشعاعي للمحاليل المائية للاحماض الامينية الالانين والفينايل الانين بوجود المرافق
مشبعة بغاز الاوكسجين او النتروجين وعند دالات حامضية مختلفة. وقد وجد ان اكسدة NADH الانزيمي
في المحاليل المشبعة بالاوكسجين NADH تنخفض بزيادة الدالة الحامضية، كذلك وجد ان ناتج اكسدة NADH
وقد استنتج من ذلك تكون جذور حرة مؤكسدة تتكون من خلال عملية تتضمن سحب الامونيا من الحامض
المقاسة في محاليل الالانين هي اعلى بقليل من تلك المقاسة في NADH الاميني. النتائج اظهرت ان اكسدة
محاليل الفينايل الالانين.

24

Construction of Multi-Electrodes Electrochemical Detection System for Flow-Injection Analysis (FIA): Application to Cu1+/Cu2+ Couple

Sadallah T. Sulaiman, Amer Th. Al-Taee

Pages: 193-200 English

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Abstract

A house-built multi-electrodes detector has been designed for flow-injection
electrochemical analysis. The detection system constructed intwo part, the auxiliary
electrode (1 mm diameter Pt wire), the working electrodes (5 mm diameter glassy carbon
(GC)), 5 mm diameter graphite (GR) and 5 mm diameter poly pyrrole plated (PPPE) as
conducting polymer) placed in one part where as the reference electrode (Ag/AgCl,sat.
KC1) placed in the second part. These electrodes were fitted into a teflon body cell, as it
is a good isolator and easy to machine. One can readily change the working electrodes
from GC, GR or PPPE without taking the cell apart. The cell based on a conventional
laminar flow or on a wall-jet mode of operation. Use of the cell is illustrated by
determination of Cu1+/Cu2+ couple on the different working electrodes. The best results
obtained using the conducting polymer with lowest detection limit 10-7 M (6.3 ppb as
Cu+2 ion).

25

Flow Injection Analysis (FIA) Technique as a Method for Determination of Hemoglobin (Total) Quantity in Human Blood Serum

Amer Th. Al-Taee

Pages: 201-211 English

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Abstract

A new method for the determination of Hemoglobin quantity in human blood serum based on Flow Injection Analysis (FIA) was described. This method is relay on the decrease in the reduction peak of Potassium Ferricyanide K3[Fe(CN)6] which appeared at (-0.5V) vs. (Ag/AgCl, Sat. KCl) using Phosphate buffer (pH= 7.0) as a supporting and carrier solution. The method is sensitive and rapid. The procedure was successfully applied to the determination of Hemoglobin in various blood samples represent normal and different diseased cases such as: anemia, polysythemia. A comparison between the proposed method (FIA) and colorimetric method was carried out using (36 samples), the relation was linear with a correlation coefficient (r= 0.9998).

Abstract

يتضمن البحث وصف طريقة جديدة لتقدير كمية الهيموكلوبين (Haemoglobin) في مصل دم الانسان باستخدام تقنية التحليل الحقني الجريائي (FIA). تعتمد الطريقة على قياس الانخفاض في قمة اختزال مادة سيانيد البوتاسيوم الحديديكي ([6(Potassium Ferricyanide K3[Fe(CN) عند جهد 0.5- فولت المستهلك خلال التفاعل الانزيمي للهيموكلوبين. تم تطبيق الطريقة المقترحة لقياس كمية الهيموكلوبين في (36) نموذج من عينات مصل الدم لاشخاص اصحاء وكذلك المصابين في حالات مرضية مختلفة مثل: فقر الدم وسرطان نخاع العظم (لدى الكبار). تم مقارنة النتائج المستحصلة من هذه الطريقة مع الطريقة الطيفية وكانت العلاقة خطية وبمعامل ارتباط (0.9998=r).

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