Abstract
The addition of PDA compound (10-6, 10-7, 10-8 M) with (10-6 M) triazoles
compounds stimulates callus formation from stem segments depending on the types of
derivative and PDA concentration used. Moreover, the addition of most triazoles
compounds (10-6 M) with PDA (10-8 M) induced the shoots regeneration in greater
numbers as compared with the presence of NAA. Most compounds added enhanced
growth of the callus similar to that grown on the standard medium or more especially in
the case of G, H and D compounds. The fresh weights of callus grown on medium
containing G and PDA were 27.5 g as compared with other compounds used.
Interestingly, protein content of callus increased with the lapses of growth period in
the same pattern as the changes in fresh weight.
The conclusion is that triazoles compounds behave in the same way as cytokinins in
the culture medium.
These compounds were more effective than the standard growth regulators in
growth and differentiation of lettuce plant.
However, the use of PDA instead of standard auxins enables to exclude the use of
imported growth regulators, which has great economic importance. This study is regarded
for the first time an attempt to use locally prepared auxins and cytokinins in Lettuce
tissue culture system.
compounds stimulates callus formation from stem segments depending on the types of
derivative and PDA concentration used. Moreover, the addition of most triazoles
compounds (10-6 M) with PDA (10-8 M) induced the shoots regeneration in greater
numbers as compared with the presence of NAA. Most compounds added enhanced
growth of the callus similar to that grown on the standard medium or more especially in
the case of G, H and D compounds. The fresh weights of callus grown on medium
containing G and PDA were 27.5 g as compared with other compounds used.
Interestingly, protein content of callus increased with the lapses of growth period in
the same pattern as the changes in fresh weight.
The conclusion is that triazoles compounds behave in the same way as cytokinins in
the culture medium.
These compounds were more effective than the standard growth regulators in
growth and differentiation of lettuce plant.
However, the use of PDA instead of standard auxins enables to exclude the use of
imported growth regulators, which has great economic importance. This study is regarded
for the first time an attempt to use locally prepared auxins and cytokinins in Lettuce
tissue culture system.