Rafidain journal of science

Manhal S. Al Annaz

Worm burden, dispersion and egg count of Echinococcus granulosus were studied
in Mosul city during 1997 to 1999. One hundred and twenty adult stray dogs were killed
and necropsied for detection and determination of the actual number and dispersion of E.
granulosus along the small intestine. Results revealed that worm loads ranged between
52 to 2000 worms with an average 1026 worm per dog. The commonest site of worms
was the second third of the small intestine (75%), followed by the first third (20%). Few
helminths were recovered in the last third which constituted 5% . The number of ova
observed in the uteri of a gravid segment was 80 to more than 3000. The findings showed
that 16.66% of the dogs harbour the cestode.

One hundred fifty patients who were included in this study visited the endoscopy
unit these complaining from epigastric pain, dyspepsia, acidity, vomiting, abdominal
pain, flatulance, heart bum and melena, All underwent esophagogastroduodenoscopy
(OGD) and biopsies were taken, on which bacteriological and histopathological tests
were done, aiming, first, isolation of Helicobacter pylori and second, looking for
documents regarding the role of this bacteria in pathogenesis of peptic ulcer. Direct
smear of biopsies were examined and then cultured on selected media of H.pylori.
Histopathological examination was also done, using paraffin and plastic sections. When
detection of H. pylori was considered, the highest sensitivity rate was found 74.1% when
it was cultured on [Brucella agar + (5-7% )sheep blood + skirrow's supplement] while
direct smear examination from biopsies was the second and it was 70%, the third position
was obtained by histopathological examination of paraffin section and it was 67.9 %, on
the contrary plastic section cames in the fourth place 43.5 %. Vacuole formation (in-vivo)
was documented on histopathological examination and it was the most important finding
as we think that may explain the role of H. pylori in peptic disease. The vacuoles then
enlarge leading to cell ruptures and cell death knowing this fact we think peptic ulcer is
caused by H. pylori.