Journal of Kerbala for Agricultural Sciences

This study had been conducted in the First Research Station for Agriculture College
Muthana University and the laboratory of biological technique & practical analysis
- Ministry of Science and Technology through the period 1/11/2015 to
1/07/2016. The aims of this study was to determine the genetic components of Leptin
gene and explore the relationship of these components with a some of production for
local Awasi sheep. The Genotypes had been different to the targeted coding area for
Leptin gene according to the variation of the genetic packs that produced by the enzymatic
digestion which had reached three genotype who are AA, AB and BB and
their distribution ratios were 50.00, 43.33 and 6.67% respectively, and the variation
between these ratios was highly significant, and the allele frequency was 0.72 and
0.28 for the allele A and B in series. The current study results shown that total milk
production for Awasi sheep had been significantly affected (P<0.05) by the genotypes
of Leptin Gene for the sheep that had pure genotype AA, as for milk period duration
it had not been significantly affected by genotype variation. The fate and nonfate
solid ratios had been significantly influenced where it had been at maximum in
sheep’s milks that had the genotype BB (6.79 ±1.34%) and AB (11.05 ± 0.29%) for
both parameters in series, while lactose and protein ratios had not been significantly
affected by the genotype variation. It can be concluded through the study of the genotype
of Leptin gene and its future it adoption to set the genetic rehabilitation strategies
for sheep to maximize their economic income of sheep genotype selection and
interaction breading projects which already achieved best economic parameters, Additionally,
the application of this study on larger sample for many production seasons
can give more accurate results to adopt exclusion and replacement strategy.

This study had been conducted in the First Research Station for Agriculture College Muthana University and the laboratory of biological technique & practical analysis - Ministry of Science and Technology through the period 1/11/2015 to 1/07/2016. The aims of this study was to determine the genetic components of Leptin receptore gene and explore the relationship of these components with a some of production
for local Awasi sheep. The genotype distribution ratios had been contrasted
TT, TV and VV for Leptin gene receptor (LEPR) significantly (P<0.01) in the studied
sample, where there ratios had reached 8.33, 55.00 and 36.67% in series with allele
frequency was 0.36 and 0.64 for each T and V allele in series. The current study
results showed that the total milk production and milk season duration period for
Awasi sheep had been significantly influenced( P<0.05 )by genotype for LPR gene
and for the sheep that had structure VV for milk and for TT genotype was the milk
season duration. The studied milk component ratio had not been influenced by the
variation of genotype except for fate ratio. It can be concluded through the study of
the genotype of Leptin gene and its future it adoption to set the genetic rehabilitation
strategies for sheep to maximize their economic income of sheep genotype selection
and interaction breading projects which already achieved best economic parameters,
Additionally, the application of this study on larger sample for many production seasons
can give more accurate results to adopt exclusion and replacement strategy.

A study was conducted to investigate the effect adding B12 to tris extender on
the physical and chemical semen characteristics, the semen storage at (5°C).five
Awassi rams 3-4 years of age all semen samples were tested for physical characters
such as(individual motility ,live and dead sperm ,abnormal sperm%, ,Hypoosmotic
test %, ALT and AST). Semen from each rams in the groups were pooled and divided into four parts to study the effect of adding different concentrations of antioxidants
B12 to the diluent of semen .The added B12, was (0,1,2,3 mg\ml) , Semen diluent
were kept in refrigerator (5°C). The results showed that there were significant
differences between the concentration (2,3, mg\ml) B12 comparison to(0,1mg\ml)
B12 in individual motility 3.00 ± 78.42 , 2.22 ± 81.13 % and 0.19 ± 75.62 , 3.17
±87.49 in live and dead sperm comparison to control 12 ±73.55 at first day, and
Hypoosmotic 0.77 ± 78.66 ,1.37 ± 81.33 comparison to control 0.45 ± 62.54ALT
and AST , 0.49 ± 27.81 ,1.19 ± 26.29 comparison to control1.34 ± 33.53 % at third
day and there were significant differences between the concentration (2,3, mg\ml)
B12 comparison to(0,1mg\ml) in all characters at fifth day.

The research was conducted to investigate the effect of supplementation of
Creatine and Carnitine with their combination on some productive traits of Ross 308
broiler chicks.
Atotal number of 450, one old chicks were used in the research , those chicks
were randomly alloted in to 9 equal treatments and each treatment included 2 equal
replicates.Chicks were fed on diets supplemented with Creatine and Carnintine.
treatments were arranged as follow: The 1st treatment fed on an ordinary diet with out any supplement where as
the 2nd and 3rd treatments fed on a diet supplemented with 100 and 200 mg/kg
Creatine respectively. The 4th and 5th treatments fed diets
supplemented with 100 and 200 mg/kg Carnintine respectively. The 6th treatment
supplemented with 100 mg / kg Creatine plus 100 mg / kg Carnintine , mean while
the 7th treatment supplemented with 200 mg / kg Creatine plus 200 mg/kg Carnintine.
The 8th treatment supplemented with 100 mg / kg Creatine plus 200 mg / kg
Carnintine and the 9th treatment supplemented with 200 mg / kg Creatine plus 100 mg
/ kg Carnintine. Chicks in the experimental treatments fed adlibitum on the starter
diet till 21 day , then transferred to the growing diet till 42 day.
Results illustrated surpassing of live body weights , weight gains as well as
improvement of feed conversion ratio with reduction of the utilized feed of broiler
chickens that fed diets supplemented with Creatine and Carnintine.

This study was conducted at Poultry Farm of Animal production Dept., College of
Agriculture, University of AL-Qasim Green to investigate Study the effect of adding
different levels of Olive Plant Leaves (Olea europea L.) powder to the ration on
productive traits of broiler chicks Ross 308 .Use the 120 broiler chicks Ross 308 dayold
were randomly assigned to four treatments (by 3 replicates per treatment 10
chicks per replicate),and treatments were as follows: Treatment for the first (control)
without adding olive Leaves powder into the ration, the added treatments were 500,
750 and 100 mg / kg for the first, second and third treatment treatments respectively The experiment included a study of the following characteristics: body weight,
weight gain, feed consumption, feed conversion efficiency and mortality.
The results indicated that the addition of olive levels for broiler chickens diet there
was not any significant effect on the productive qualities of broiler chickens

A Field experiment was carried out during the winter season of 2015-2016 at Al-
Miqdadiyah-Diyala province to study effect of salicylic acid in growth and yield of
wheat (Adna 99 cultivar) under drought conditions. Arandomized complete block design
by Split Plot arrangement was used with three replications. The main plots included
three levels of drought stress 50%, 75% and 100% from the recommended
water. The sub plots included four concentrations of salicylic acid applied 0, 100,
200, and 300 part per million. The results showed that all the studied characteristics
of growth, yield and yield components were significantly reduced by increasing
drought levels. Plant height, number of tillers, flag leaf area, dry weight of plants,
number of spikes number of grain per spike, weight of 1000 grain, Grain yield, biological
yield and harvest index were decreased. Lowest values recorded by 50% from
recommended water. While 100% from recommended water recorded highest values
82.89cm, 610.6 tiller.m-2 , 41.07cm, 1280.9 gm.m-2 , 521.7spike.m-2 , 49.31grain
.spike-1 , 33.24 gm, 8.52 ton . h-1 , 24.35 ton . h-1 and 35.02% respectively. Salicylic
acid improved all of studied characteristics except number of spikes. The concentration
100 part per million was the best concentration, which recorded 80.35 cm,
571.13 tiller.m-2, 39.63cm2, 1207.7gm.m-2 ,45.81 grain.spike-1 , 33.90 gm,7.58 ton .h-1
, 22.18ton .h-1 and 33.99% , respectively. It was concluded that drought stress reduced
growth and grain yield. Salicylic acid improved growth of plants and caused
increasing in grain yield with and without drought conditions.
Key Words. Salicylic Acid, Drought, Wheat

This experiment was conducted in the Lathhous of the Technical Instituite \
Musaib during 2016 growth season on sour orange seedling, Which included two
Factor : First was study the influence spraying (Grow green) in four levels (0 , 3 , 6
and 9) mg.L-1 and the second factor was study the effect of four levels of spraying
with Salicylic acid (0, 50 , 100 and 150) mg.L-1 and their interaction on vegetative ,
root traits , leaves content of chlorophyll , Nitrogen , Phosphor and Potassium. The
experiment in complete design of randomize (CRD) was done with three replicates
and five Seedling for each treatment we have got the following results :
Spraying solution (Grow green) at 9 mg.L-1 gave the highest means of all characters
studied and the content of chlorophyll and (N , P , K) in the leaf. Spraying solution (Salicylic acid ) at 150 mg.L-1 caused highest mean of traits studied. Interaction between
the treatment 9 mg.L-1 (Grow green ) Sapling with 150 mg.L-1 Salicylic acid
gave the highest values of studied characters.

Experiment carried out at the Technical Institute of AL-Musayaib during the grow
season 2014 on the seedlings of prunus mrianna .When were sprayed two types of
foliar feeding the first factor spray seedling foliar feeding Black sea concentnations
(0,0.5,1) g /L1- .the second factor was the concentration of foliar feeding Elodia
(0,50 ,100,150) m/L1- the experiment as a factor random complete design with three
replications and by three seedlings of per treatment and the results showed the following
:--Excellence level 1g/L1- of compost Blaek sea outweigh moral to than treatments
and in all of the traits which gave respectively 124.91 and 12.47 and 116.15 and 26.93 and 17.94 and 33.66 and 1.23 plant height and number of branches ,number of
leaves and area and weight dry and the proportion of the chlorophyll and the percentage
of nitrogen .- showed the level 150 ml/L1-of fertilizer Elodia significant superiority
on all treatment which gave the 127.62 and 13.14 and 123.46 and 23.98 and
18.13and 35.25and 1.27for recipes studied and respectively plant height and number
of branches ,number of leaves and leaf area and dry weight ratio chlorophyll and the
percentage of nitrogen .pravaleaya moral synthesis between (Black sea ,Elodia ) to
give the high average growth for all growth indicators which gave the 132.14 and
138.01and 14.31 and 26.04 and 19.29 and 39.15 and 73.10 and respectively .

Two experiments was conducted. The first experiment is laboratory, by using
the Columns techniques to evaluate the effectiveness of Gypsum Phosphate as a
chemical mending. The second experiment is a biological to evaluate the efficiency
of Gypsum Phosphate to improve the qualities of the soil cultivated flora of barley
which irrigated by the saline water of the main out full at Abu-Ghariab location. 0,50
and 100 ton . ha-1 of Gypsum Phosphate was added at two replicate then irrigated water
given at 5000 m3. ha-1. Results of chemical analysis tests of the soil columns
(colums technique) showed and proved using Gypsum Phosphate efficiency as good
chemical mending at saline water as important calcium resource, as well as Gypsum
Phosphate addition in columns technique leads to reduce EC and SAR in soil to a
limit value which allowed for optimal growth of barley plants, also leads to reduce
absorption of Sodium Na+ by plants roots and thus improve the most nutrient absorption,
which reflected positively in increasing plant growth. Also increasing of Calcium
concentration in columns Cause led to the removal of agreater amount of sodium
out of the roots zone and thus improving the qualities of soil chemical,physical and
fertility. Results also indicate that the optimal level of Gypsum Phosphate addition
was at level 50 ton.ha-1. And the higher level of it was unsignificant economically.

The study was conducted elwing two growing seasons (2015 and 2016) in aprivate
farm in Kufa-Najaf,with the objective of improving salinity tolerance of tomato (Lycopersicon
esculentum Mill.) with exogenous application of silicon. The study included
40 treatments, whil were four levels of irrigation water salinity (2.5, 5.0, 7.5
or 10 ds.m-1) , two tomato cultivars (Aulla and Majid) and five concentration of silicon
(0 , 50 , 100 , 150 , or 200 mg l-1). Asplit-split plot design was used in afactorial
experiment with three factors and three replicates. The results were analyzed using
analysis of ariauce and treatment means were comparod using the least significant
differences (LSD) at 0.05. The effect of the above treatment were studied on vegetative
growth characteristies were plant length ,number of side shrots,leaf number,leaf
area as well as dry weights of the shrot sytem, Irragation water salinity at 7.5 and
10.0 ds m-1 caused a significant decrease in vegetative growth characteristies for
both growing seasons. Exogenous treatment with silicon caused a significant increace
in all vegetative growth characteristies, for both growing seasons. As for the effect of
cultivars, the cultivar Majid was superior in all vegetative growth characteristies, as
compred with cv. Aulla, for both growing seasons. As for interactions among treatments,
they were significant in thair effects on vegetative growth characteristies and
for both seasons.

A field study has been conducted during autumn season of 2016 at Karbala
province of Hindia by mungbean crop Vigna radiate L. to study the interrelated
effect of both the Mycorrhizal fungi (Glomus mosseae ( and the Rhizobial bacteria (
R.leguminosaru( Under levels of water stress. Three treatments of irrigation are used
; (S1 irrigation every 5 days and S2 irrigation every 10 days and S3 irrigation every
15 days) and four levels of Bio fertilizer are used (without inoculation)(C0) and
(Rhizobium inoculation)(R) and (mycorrhizal inoculation)(M) and (the interaction
between mycorrhizal and Rhizobium)(M+R). A split plot in randomized complete
block design is used with three replications. Treatments of irrigation are used as main
plots while Bio fertilizer levels are used as sub-plot. Least significant difference
(LSD) at 5% probability is used to compare the means. The results showed irrigation
every 5 days (S1) was superior in having the highest average of (plant height, leaf
area, relative water content of leaves, root length, dry weight of roots, and infection
Mycorrhizal inoculum) Amount (50.63 cm. Plant-1, 576.4 cm2. Plant-1,74.68
%,17.79 cm. Plant -1 , 0.78 gm. Plant-1, 58.9%) respectively. While Biofertilization
treatment (mycorrhizal and Rhizobium)(M+R) showed its superiority in all
characteristics. The interaction among (R+M)S1,(R+M)S2 and (M)S1 significantly
give the highest means for all plant characteristics.

The current experiment was conducted to study the effect of proline and glycine
betaine at two concentrations for each; 50 and 100 mM in addition to control and two
salts; sodium chloride and calcium chloride at 25 and 50 mM for each in addition to
control, in vegetative characters of Washington navel orange (Citrus sinensis L.)
grafted on volkamer lemon or swingle citrumelo rootstocks. Salts were added to the
irrigation water and seedlings were irrigated with saline water three times at three
month interval. After one week of the irrigation with saline water, seedlings were
sprayed with the osmoprotectants (three sprays). Treatments were arranged in RCBD
design as a factorial experiment(2*5*5)with three replications.
The results revealed that the two rootstocks had no effect on the content of the
leaves from Auxin , Gibberellic Acid and some antioxidants on swingle citrumelo
rootstocks. The use of the two protectants at all concentrations caused significant
increase in content of the leaves from Auxin , Gibberellic Acid. Glycine betaine was
superior in its effect compare to proline. Also, the two salts had inhibitory effects on
most of the parameters measured. The combination between rootstocks and
osmoprotectants had significant effect on most growth parameters. In general, most
of the two combinations had pronounced effects. In addition, the combination of the
three factors also effect the parameters significantly. From the above results, we can
conclude that the two salts had an inhibitory effect on the leaves content of Auxin ,
Gibberellic Acid and some antioxidants which can be improved by the addition of
glycine betaine and proline.