Abstract
Spodoptera litura causes significant damage to plants and disruption the production process of Jatropha curcas seeds up to 70% if not controlled in attacks above the economic threshold. S. litura control is now beginning to be constrained by S. litura resistance. The suspected resistance is due to the usage of synthetic pesticides that not controlled. entomopathogenic bacteria are important biocontrol of S. litura pests that support sustainable plantations. J. curcas plant is an alternative biodiesel producer with a potential seed oil content of 47.58%. Currently, the exploration of local entomopathogenic bacteria from the rhizosphere J. curcas hasn\'t been done. This study uses exploration and descriptive methods by analyzing data characterization of entomopathogenic bacteria through analysis using the website PBSTAT-CL 2.1.1 https://apps.pbstat.com/reports/pbstat-cl/. The effectiveness of bioassay in analysis using IBM SPSS Statistics 26 software, with probit analysis (lethal time 50) LT50. This research obtained 34 isolates of entomopathogenic bacteria rhizosphere J. curcas with 6 groups of characterization. The maximum cophenetic distance at 0.7 from isolates. Effectiveness from all isolates reached 100% mortality and the analysis results of LT50 probit fastest for 31,645 hours.