Samarra Journal of Pure and Applied Science

The rapid development in laser technology and the understanding of its applications and biological reactions have contributed to broadening new horizons in the field of drug treatment. In this study, an aqueous solution with a concentration of 1 M was subjected to a diode laser with a wavelength of 808 nm and a power of 1 W. This solution was exposed to the laser for three different periods: 10, 15, and 20 seconds, with the sample fixed at a distance of 10 cm. Studying some of the optical properties of the solution revealed that its absorbance increased by 3.5% at the longest laser exposure period. This led to enhancing the qualitative characteristics of the drug used.

In this work, used the green method to preparing the silver nanoparticles (AgNPs) by using Citrus Limon Extract. The novel approach has been carried for the green synthesis of AgNPs by using different parts from Limon. Using X- RD to study the structural properties of samples and calculate the crystallite size then study the effect of deference parts of Citrus Limon plant. appear from the results change in the crystallite size, that because the extract influence, and found changed depending on the type of extract (12.6,15.4,17.2 nm) when use (Seeds, Leaves, peels extract) respectively, also the results were analysis by use (AFM) atomic force microscopy, the results appear improving in structural properties by using the plant extract. Optical properties (Absorption and Transmittance) also investigated, appear dependence the optical properties of the prepared samples on the extract type, higher absorption when used seeds extract to preparing Ag Nps. Appeared from the results high antibacterial activity of AgNPs to both positive and negative gram. The inhibition zone of AgNPs depends on crystal size and the concentration of AgNPs, for that the inhibition zone radius increases with decrease the grain size. To evaluation the antibacterial activity of AgNPs against three kind of Gram-positive bacteria (E. faecalis, Staph.hominis and Staph.aureus), proved that Citrus Limon extract improved the performance of nanoparticles and found the highest inhibition diameter was 45 mm against Staph.hominis , wheen against types of Gram-negitive bacteria (Entero.cloacae, E.coli and K.pneumoniae) found the highest inhibition zon 40mm againstStaph.hominis, wheen less inhibition zone 14 mm against E.coli.