Abstract
This study was conducted in the saran-covered canopy belonging to the Department of Horticulture and Landscape Architecture, College of Agriculture and Marshes, University of Dhi Qar, to evaluate the efficacy of foliar melatonin application in enhancing the drought tolerance of China rose (Hibiscus rosa-sinensis L.) seedlings subjected to water stress induced by prolonged irrigation intervals. The experiment was implemented as a two-factor completely randomized block design (RCBD) with three replicates. The first factor comprised three irrigation regimes: daily irrigation (control), irrigation every four days (moderate drought), and weekly irrigation (severe drought). The second factor consisted of three exogenous melatonin concentrations: 0, 50, and 100 mg L⁻¹. Quantitative and qualitative data were statistically analyzed using the Least Significant Difference (L.S.D.) test at a significance threshold of P≤0.05.
The results demonstrated that water deficit induced by expanding irrigation intervals caused a statistically significant decline across all evaluated vegetative and physiological traits. Specifically, plant height, leaf count, total leaf area, stem diameter, and total chlorophyll content systematically decreased with rising drought severity. Conversely, leaf chlorosis and tissue damage percentages escalated significantly under water stress, while the healthy green leaf area percentage dropped sharply. The weekly irrigation treatment recorded the lowest structural and biochemical values; total chlorophyll content dropped by over 46% compared to the daily irrigated control, while chlorosis and cellular damage reached their peak levels.
In contrast, exogenous foliar spray with melatonin achieved significant and substantial ameliorations in all studied parameters, particularly at the maximum concentration of 100 mg L⁻¹. This treatment markedly increased plant height, leaf number, functional leaf area, stem diameter, and chlorophyll synthesis, while successfully minimizing chlorosis and tissue necrosis percentages. Furthermore, the dual interaction between irrigation intervals and melatonin treatments demonstrated that daily irrigation combined with 100 mg L⁻¹ melatonin optimized overall seedling performance. Crucially, melatonin application effectively mitigated the deleterious impacts of water deficit even under the most severe weekly irrigation regime. The study concludes that melatonin exerts a vital protective, signaling, and physiological role in bolstering the drought tolerance of Hibiscus rosa-sinensis L. by preserving photosystem integrity, maintaining cellular turgor, improving growth efficiency, and reducing oxidative cellular damage caused by severe water deficit