Abstract
b
ackground: Escherichia coli is a frequent cause of urinary tract infections,
however, its identity as pathogen in the cervico-vaginal area is required to be
ascertained. In addition, source (s) for E.coli colonzing female vagina is needed to
be confirmed, whether its fecal contamination or from urinary tract.
Aim of the Study: To perform a comparative analysis of the E. coli clinical isolates from
vagina versus those from urine in terms of molecular phylogeny, molecular determinants of
virulence and antimicrobial susceptibility.
Materials and methods: A total of 60 E. coli strains from high vaginal swabs (n=30) and
urine (n=30) were analyzed. Identification of phylogenetic groups and detection of adhesive
genes were conducted by 2 different multiplex PCR systems. Antibiograms for all isolates
were performed by Kirby-Bauer method.
Results and Discussion: Majority of vaginal E coli (VEC) isolates were belong to B2
phylogenetic group (n=20, 66.7%), whereas, majority of uro-pathogenic E. coli (UPEC)
isolates were distributed between two phylogenetic groups, namely B2 12 (40%) and D 11
(36.7%). Therefore, most of the strains from both vagina and urine are belonging to
pathogenic phylogenetic groups; however, they differ in prevalence of the groups. The pap
gene has a higher frequency among UPEC (n= 13, 43.3%) than in VEC isolates (n=7,
23.3%). Similarly, sfa gene has a higher frequency in VEC isolates (n= 20, 66.7%) than in
UPEC isolates 11 (36.4%). Consequently, adhesion genes playing roles in vaginal
colonization may differ from that in urinary tract .VEC strains where highly susceptible to
ciprofloxacin (100%) followed by nitrofurantoin (73.3%) and nalidixic acid (70%). Whereas
UPEC strains were highly susceptible to nitrofurantoin (100%) followed by nalidixic acid.
Thus, it seems that cirpofloxacin is appropriate for empirical therapy in vaginal infections,
whereas nitrofurantoin is more appropriate for empirical therapy in UTI.
Conclusion: Strains isolated from high vaginal swabs differ from strains isolated from urine
in the prevalence of phyelogenetic groups andmolecular determinants of virulence as well as
in antibiograms.
ackground: Escherichia coli is a frequent cause of urinary tract infections,
however, its identity as pathogen in the cervico-vaginal area is required to be
ascertained. In addition, source (s) for E.coli colonzing female vagina is needed to
be confirmed, whether its fecal contamination or from urinary tract.
Aim of the Study: To perform a comparative analysis of the E. coli clinical isolates from
vagina versus those from urine in terms of molecular phylogeny, molecular determinants of
virulence and antimicrobial susceptibility.
Materials and methods: A total of 60 E. coli strains from high vaginal swabs (n=30) and
urine (n=30) were analyzed. Identification of phylogenetic groups and detection of adhesive
genes were conducted by 2 different multiplex PCR systems. Antibiograms for all isolates
were performed by Kirby-Bauer method.
Results and Discussion: Majority of vaginal E coli (VEC) isolates were belong to B2
phylogenetic group (n=20, 66.7%), whereas, majority of uro-pathogenic E. coli (UPEC)
isolates were distributed between two phylogenetic groups, namely B2 12 (40%) and D 11
(36.7%). Therefore, most of the strains from both vagina and urine are belonging to
pathogenic phylogenetic groups; however, they differ in prevalence of the groups. The pap
gene has a higher frequency among UPEC (n= 13, 43.3%) than in VEC isolates (n=7,
23.3%). Similarly, sfa gene has a higher frequency in VEC isolates (n= 20, 66.7%) than in
UPEC isolates 11 (36.4%). Consequently, adhesion genes playing roles in vaginal
colonization may differ from that in urinary tract .VEC strains where highly susceptible to
ciprofloxacin (100%) followed by nitrofurantoin (73.3%) and nalidixic acid (70%). Whereas
UPEC strains were highly susceptible to nitrofurantoin (100%) followed by nalidixic acid.
Thus, it seems that cirpofloxacin is appropriate for empirical therapy in vaginal infections,
whereas nitrofurantoin is more appropriate for empirical therapy in UTI.
Conclusion: Strains isolated from high vaginal swabs differ from strains isolated from urine
in the prevalence of phyelogenetic groups andmolecular determinants of virulence as well as
in antibiograms.