Abstract
Background: Measles is an extremely contagious disease characterized by generalized maculopapular rash, fever, cough, coryza, and
conjunctivitis. It is caused by the measles virus. Objective: The objective of this study was to explore the anti-measles immunoglobulin (Ig)
M positivity rate among children with clinical suspicion of having measles. Subjects and Methods: This is a cross-sectional study carried
out in Diyala Province, Iraq, from November/2020 to October/2021. A total of 425 blood samples were collected from children (≤14 years
of age): 90 from patients who were clinically suspected as having measles (18 were vaccinated with measles vaccine and 72 were not);
270 from measles-vaccinated children, including those children who received all recommended vaccines in the Iraqi Expanded Program of
Immunization (IEPI); and 65 from nonvaccinated children, including those children who received none of the vaccines in the IEPI. Enzyme-linked
immunosorbent assay (ELISA) kits for the detection of anti-measles IgM were used. Results: The results found that 14 (15.6%) of clinically
suspected children were positive for anti-measles IgM antibody versus 76 (84.4%) who were negative. Whereas, only one (1.5%) of the
unvaccinated children was positive, and all vaccinated children were negative. Thus, clinically suspected patients had a significantly higher
positivity rate compared to other study groups (P = 0.0001). Similarly, the mean ± standard deviation of anti-measles IgM concentration was
significantly higher compared to other study groups (P = 0.0001). Conclusion: About one-sixth of patients clinically comparable to measles
in Diyala Province actually had measles, most of them were unvaccinated, and the anti-measles IgM ELISA technique was a good marker
for exploring measles cases.
Keywords: Anti-measles immunoglobulin M, Diyala, measle
conjunctivitis. It is caused by the measles virus. Objective: The objective of this study was to explore the anti-measles immunoglobulin (Ig)
M positivity rate among children with clinical suspicion of having measles. Subjects and Methods: This is a cross-sectional study carried
out in Diyala Province, Iraq, from November/2020 to October/2021. A total of 425 blood samples were collected from children (≤14 years
of age): 90 from patients who were clinically suspected as having measles (18 were vaccinated with measles vaccine and 72 were not);
270 from measles-vaccinated children, including those children who received all recommended vaccines in the Iraqi Expanded Program of
Immunization (IEPI); and 65 from nonvaccinated children, including those children who received none of the vaccines in the IEPI. Enzyme-linked
immunosorbent assay (ELISA) kits for the detection of anti-measles IgM were used. Results: The results found that 14 (15.6%) of clinically
suspected children were positive for anti-measles IgM antibody versus 76 (84.4%) who were negative. Whereas, only one (1.5%) of the
unvaccinated children was positive, and all vaccinated children were negative. Thus, clinically suspected patients had a significantly higher
positivity rate compared to other study groups (P = 0.0001). Similarly, the mean ± standard deviation of anti-measles IgM concentration was
significantly higher compared to other study groups (P = 0.0001). Conclusion: About one-sixth of patients clinically comparable to measles
in Diyala Province actually had measles, most of them were unvaccinated, and the anti-measles IgM ELISA technique was a good marker
for exploring measles cases.
Keywords: Anti-measles immunoglobulin M, Diyala, measle