Al-Qadisiyah Medical Journal

A total of eighty nine clinical samples were collected from pacifier of children patients with age1-48 months from both gender complaining from diarrhea in Hilla city. 19 isolates of E.coli were isolated and identified according to their morphological properties( cultural and microscopical) and biochemical tests and percentage (21.3%).Some clinical samples were showed Entamoeba histolytica ,Giardia lamblia and Trichomonas hominis and Monilia. The susceptibility of the all current isolates were carried out against 17 antibiotics, E.coli isolates were very sensitive to meropenem and impenem ,and sensitive to sefitrixon ,amikacin, and ciprofloxacin ,while its resistance to others antibiotics were different. The minimum inhibitory concentrations(MIC) of some present isolates were higher.The combination of - lactam antibiotics (amoxicillin and cefotaxim)with quinolones(ciprofloxacin)from side and aminoglycoside (amikacin) from other side , demonstrated effective synergism of these mixtures anti multidrug resistance E.coli isolates.

Abstract
The current study included the detection of Toxoplasma gondii from aborted women attented to the Maternity and Childhood Teaching Hospital to detected the rate of abortion due to toxoplasmosis using PCR technique, the samples which was used placenta and fetus, this study also identify the differentiation between it by phylogenic with SAG3 gene.
A total of 60 ( placenta and fetus) samples were collected randomly from aborted women who received to the Maternity and Childhood Teaching Hospital.
The present study include molecular method to detected small subunit ribosomal RNA ( ssRNA) using Nested Polymerase Chain Reaction ( nPCR) ,the results of the nPCR for ssRNA gene, the result showed that the rate of toxoplasmosis in the aborted women was Positive 60%. (There is a statistically significant between species in P <0.05).
The present study also detected for Surface Antigen (SAG3) gene to conform the nPCP result and to send the samples for sequences.
The SAGA gene showed 100% positive results according to the ssRNA gene .
T.gondii isolates surface Ag SAG3 gene with NCBI BLAST Toxoplasma gondii isolates, and the DNA nucleotides sequencing analysis of surface Ag SAG3 complete gene was show clear gentic variation between isolates from different host.

AIM: comparison between the central corneal thickness (CCT)and corneal endothelial structure in type II diabetics and non-diabetic control patients.
METHODS: this observational study was performed in Diwanyia teaching hospital in which 350 persons ( 190 type II diabetic patients and 160 control subjects) were included. Specular microscopy and pachymetry were used to measure endothelial cell density, hexagonality, size, coefficient of variation as well as corneal thickness. Independent tests were used to compare variables between diabetics and controls.
RESULTS: Endothelial cell density in the diabetic group (2459± 244 cells/mm2) was significantly lower than that in the control group (2720± 129 cells/mm2,). The central corneal thickness (CCT) was higher in diabetics (541 ±32.8 micrometer) in comparison to control group (524±44.2 micrometer).
CONCLUSION: Type II diabetes causes a significant alteration in the state of the cornea including reduction in endothelial cell density and increased central corneal thickness.

Abstract:
Background: urinary tract contamination will be a standout amongst the as a relatable point clinical sequelae of diabetes mellitus.
Objective- this consider pointed with figure out those impact about poor glycaemic control Likewise resolved Toward glycated hemoglobin (HbA1c) levels on the predominance from claiming urinary tract infections in diabetic Patients.
Materials Furthermore Methods: in this cross-sectional spellbinding study, 50 diabetic patients going to the diabetes facility from claiming Al-Diwaniyah educating support hospital, were included as subjects What's more 25 aged and its Furthermore sex-matched Obviously sound people were included as controls. Results: mean HbA1c levels On diabetic women were altogether higher over On solid controls (p< 0. 05).
Conclusion: HbA1c values demonstrating to poor glycaemic control might make a proxy pointer to screening to UTI Around men for diabetes mellitus.