Baghdad Journal of Biochemistry and Applied Biological Sciences

Cancer is one of the most common diseases around the world and the second leading cause of death after cardiovascular disease. Breast cancer is the most prevalent cancer type among Iraqi women, as it represents the highest percentage of malignant tumors in women until 2018. Therefore, women should be aware of the aggravation of this disease, the importance of the periodic examination for early detection for breast cancer, and following the most appropriate means for the treatment to get recovered and, thus, to reduce mortality. To fight cancer, there is an urgent need to search for new effective anticancer therapies that alter the molecular biology of tumor cells, stimulate the immune system, or specifically deliver chemotherapy factors directly to cancer cells without affecting normal cells and reducing the side effects of treatments. In this context, this paper aimed to highlight the therapeutic approaches used in the current researches of breast cancer treatment. Accumulated evidence showed that medicinal plants extracts, virotherapies, and nanomaterials can serve as anticancer agents. The proposed mechanisms were discussed and presented in this review.

Background and objective: The current study aims to evaluate the glycemic index of local honey and date intake in healthy adults.
Methods: The study was conducted on 24 healthy volunteers. They were given 50 g of carbohydrates from Haji Omeran local honey, Khudri (or Khadrawi) local date and reference food (white bread). The average body mass index and age of volunteers are almost similar. The blood samples were collected from finger capillaries to assess the glucose levels by using glucometer at selected time points (0, 15, 30, 60, 90, and 120 minutes). The data was recorded and statistically analyzed by one-way ANOVA and Duncan comparison.
Results: The study results showed that the glycemic index of the honey and date is classified as a medium glycemic index, referring to their high carbohydrates content. The results also indicated that honey and dates have significantly different effects (P<0.05) on the blood glucose responses compared to reference food (white bread) in healthy subjects. The mean blood glucose levels after honey ingestion were higher than that when the date was ingested and lower than that of the reference food. In addition, the blood glucose responses for different foods in the study for females and males are not significantly different (P>0.05).
Conclusions: In conclusion, eating behavior regarding honey and date consumption, particularly portion size and ingestion timing, is essential to manage blood glucose levels. Thus, glycemic index values should be considered in promoting a healthy lifestyle from chronic related metabolic disorders especially, diabetes mellitus, cardiovascular disease, and obesity.

Background and objective: The pre-analytical rejection rate is the proportion of samples rejected at the stage that includes the initial procedures of the testing process performed outside the laboratory walls by healthcare professionals. This study aimed to evaluate the pre-analytical rejection rate by considering the health status of the patients and the sample types and to examine the measures that can be taken against it. 

Methods: The data of the samples that came to the laboratory for analysis for one year were included. These data were categorized according to sample types in complete blood count, biochemistry, hormones, urine, blood gases, coagulation, erythrocyte sedimentation rate (ESR), glycosylated hemoglobin (HbA1c). It was also categorized by emergency, outpatient, inpatient, and critically ill status. Considering the health status of the patients, the pre-analytical rejection rates determined in these sample types were compared. 

Results: Complete blood count (0.40%) in emergency patients, HbA1c (0.78%) in outpatients, biochemistry (0.62%) in inpatients, hormones (0.29%), urine (6.19%) blood gases (1.03%), coagulation (1.26%), ESR (3.23%) in critical patients, sample types had the highest pre-analytical rejection rate. 

Conclusions: The source of causes that affect pre-analytical rejection rates, such as hemolyzed sample, clotted sample, or insufficient sample, may be due to the patient's bed rest, critical or emergency. An underlying disease, treatment, or frequent phlebotomy may also be a factor. The source of the causes that affect the pre-analytical rejection rates, such as incorrect request, incorrect registration, and incorrect tube, can usually be attributed to non-laboratory healthcare personnel.

Background and objective: The most supported theory for Nephrotic Syndrome (NS) etiology is that it is immune-mediated. This study aims to assess the level of serum IgE in children with Steroid Sensitive NS (SSNS) at relapse and remission, and its correlation with the presence of atopy.
Methods: This cross sectional study was approved by the Department of Pediatrics, College of Medicine, Al-Nahrain University (Baghdad, Iraq) and conducted at Al-Imamain Al-Kadhimain Medical City (Baghdad, Iraq), and Child Central Teaching Hospital (Baghdad, Iraq), and included 31 children SSNS. The data collected was: age, sex, residency, onset of NS, response to steroid, frequency of relapses, and the history of atopy of the patient and his relatives. Serum IgE level was measured during relapse for all patients and for 9 patients while in remission.
Results: Atopy was present in 18 (58.06%) of patients. The median serum IgE level was 295.5 IU/mL (range 54-2864 IU/mL) in relapse, which is significantly higher (P-value =0.006) than in remission 228.5 IU/mL (range 62-2069 IU/mL). Median serum level of IgE in patients with atopy was 290.5 IU/mL (range 24-2864 IU/mL) which was higher than that of patients without atopy (median 231 IU/mL, range 23-1314 IU/m) (P-value = 0.029). Patients required longer period to respond to steroid therapy (>10 days) had a significantly higher median of IgE (341 IU/mL) than those who required <10 days to respond (161 IU/mL) (P-value =0.045).
Conclusions: Increased IgE level is documented during relapse and in atopic children with SSNS. Longer duration to respond to steroid therapy is associated significantly with higher serum IgE during relapse.

Background and objective: Serum beta-2 microglobulin (β2M) has been used as a useful clinical marker of chronic kidney dysfunction. The current study aims to evaluate the diagnostic accuracy of β2M for the early detection of diabetic nephropathy among Iraqi patients with type II diabetes mellitus.
Methods: The study included 84 participants divided into four groups, three of them were type II diabetics and the fourth is the healthy individuals’ (control) group. The diabetic’ subgroups were named according to the Micral test as: normoalbuminuria (21 patients), microalbuminuria (21 patients), and macroalbuminuria group (21 patients). The control group included 21, age- and sex-matched, healthy participants. Biochemical markers for diabetes mellitus as well as β2M were determined for each participant and then were analyzed statistically.
Results: The serum β2M of normoalbuminuria group was (2.86±0.95 µg/mL), microalbuminuria group was (5.06±1.97 µg/mL) and macroalbuminuria group (3.6±1.59 µg/mL). The results showed significant increase (p˂0.05) in the β2M level of microalbuminuria group when compared with that of normoalbuminuria and macroalbuminuria groups. In addition, a highly significant increase (p˂0.01) in β2M concentration was observed in microalbuminuria group when compared with that of the control group.
Conclusions: β2M can be used as a useful biomarker for the early detection of nephropathy.

Background and objective: Cigarette smoking is a leading cause of a wide range of critical health problems such as cancers, especially those who are related to the respiratory system. Although studies are continuing on the smoking-related inflammatory responses, limited reports are there to explore how such responses can be affected by the smoking intensity. Therefore, the current communication aimed to shed light on how smoking and smoking intensity can affect some inflammatory and anti-inflammatory biomarkers.
Methods: A total of 159 subjects (108 smokers and 51 non-smokers) were enrolled in this cross-sectional study. Their sociodemographic, smoking intensity and blood samples were obtained and processed using approved methodologies. The blood plasma samples were used to quantify interleukin 6 (IL-6), IL-10, tumor necrosis factor-alpha (TNF-α), C-reactive protein, D-dimer, and ferritin by using ELISA. The gained data was then analyzed using GraphPad Prism software to assess the variations.
Results: Both IL-6 and TNF-α are elevated markedly (p<0.001) in smoker subjects when compared with non-smoker ones (IL-6: 2.58±0.98 vs. 1.858±0.6256 pg/ml, TNF-α: 28.38±7.162 vs. 22.64±7.257 pg/ml). However, no significant differences were observed in other biomarkers comparing the groups, as well as no significant association was evidenced based on smoking intensity among smokers.
Conclusions: The findings might point to a relationship between smoking and the elevation of IL-6 and TNF-α levels in a cigarette dose-dependent manner.