Kufa Journal For Veterinary Medical Sciences

The aim of this study is to investigate the deferoxamine effect on rat fetal bone formation and on certain regulating genes expression that play a vital key role in bone formation. Sixty albino adult female rats three months old with average weight 170-200g were employed in this study. The rats were distributed randomly in to two groups. Control group (n=30) receiving 0.3ml ip physiological saline and deferoxamine methane intra peritoneal daily at a dose of 100 mg/kg throughout the pregnancy from the first day of pregnancy until last day of pregnancy. Skeleton samples and staining were done on sixty fetuses were chosen from each group at 20 days of pregnancy as well as liver samples from mothers and their fetuses for studying gene expression of Msx1 and Cx43. The results showed that many variations in certain bone formation and development markers were recorded as well as there was downregulation of studied genes. In conclusion, the study found that deferoxamine affect negatively on certain bone development markers and on gene expression of Msx1 and Cx43 represented as downregulation.

The goal of this study was to assess the pain-relieving action of tramadol alone or when administered with chlorpromazine and screening for analgesic effects by using sedative non-analgesic doses of tramadol and chlorpromazine when injected together in chicks. the analgesic ED50 of tramadol was 0.8 mg/kg body weight ( intra muscular injection), and the sedative ED50 of chlorpromazine was 8.6 mg / kg of body weight (intraperitoneal injection). Based on Isopolographic analysis, the type of drug interaction between tramadol and chlorpromazine was determined when injected together in a ratio by 1: 1, 1: 0.5 and 0.5: 0.5 from the median effective dose(ED50 )of tramadol and the median effective dose of chlorpromazine was synergetic interaction. Thus conclude that combination of tramadol and chlorpromazine with sedative non-analgesic doses is ideal for a good analgesic effect of pain in chicks

This study was designed to investigate the ameliorative role of gallic acid (GA) and N-acetylcysteine (NAC) in reducing deleterious effect of sodium fluoride (NaF) such as, oxidative stress and hepatic dysfunction in adult male rats. Thirty adult male rats were randomly and equally divided into five groups, they were handled daily for 60 days, as follows: Control group (C), received tap water only, Sodium fluoride group (T1), received 100ppm of NaF in drinking tap water, gallic acid group (T2), rats in this group were injected intraperitoneal (i/p) 150 mg/kg/day/ of GA, N-acetylcysteine group (T3), animals in this group were administrated orally 25 mg/kg/day/of NAC, while the combination of GA and NAC were given to NaF treated group(T4) in the same previous mentioned doses and method of administration. Fasting blood sample were collected at the beginning and the end of the experiment and serum were collected for estimation of hepatic enzymes concentration and antioxidant status. After animal scarifying, samples from hepatic tissue were taken for measuring hepatic reduced glutathione (GSH)and malondialdehyde (MDA) concentration. The results showed that adminstration of NaF (T1group) caused hepatic damage manifested functionally by: significant increase in serum alanine aminotransferase (ALT) and alkaline phosphatase (ALP) concentrations, a case of oxidative stress as explained by depression in (GSH) and elevation in MDA concentration in serum and hepatic tissue. The current result also recorded that i/p injection of GA oral administration of NAC alone or in combination with NaF caused amelioration of all previously estimated parameters.

This study is to investigate Brucellosis, Toxoplasmosis and Foot and Mouth disease of buffalo herds in Baghdad villages. 114 samples (serum) were collected, including 22 for calves less than 6 months and 92 samples of females aged between three to seven years, the samples were collected by staff of Baghdad Veterinary Hospital / Veterinary Directorate, and has been working in Central Veterinary Laboratory/ Veterinary Directorate. The ELISA diagnostic kits are selected for the detection of antibodies of Brucella abortus, Toxoplasmosis and Foot and Mouth disease (Multispecies). The result of the research is the presence of different levels of infection in the same herd 58.18% of Brucella abortus, 11.40% of Toxoplasmosis and 44.73 % of Foot and Mouth disease (Multispecies). The purpose of the study is to investigate the three diseases and their compatibility with the symptoms, although buffalo herds have not been previously vaccinated against the diseases, which proves the existence of different levels of infection in one herd and it adversely affect the development of livestock.