Abstract
Silver nanoparticles (AgNPs) coated with polyvinylpyrrolidone (PVP) was prepared by pulsed laser ablation method in
PVP solution, 1000 pluse was used with laser energy of 800 mJ/pulse. Formation of AgNPs-PVP was confirmed by UV visible spectrophotometer detecting surface plasmon resonance chang in the abilation solution. Concentration of AgNPs, size distribution, and surface charge of the ablated AgNPs in the produced solution was determined using atomic absorbtion spectroscopy and zeta potential analysis respectivly. Nanoparticles shape was determined using transmission electron microscope imaging. Ability of the senthezied AgNPs to inhibit the growth of cancer cells was compared to that of normal fibroblast cells using two cancer cell lines (HeLa and SKOV-3) in different concentrations (0.78, 1.56, 3.125, 6.25, 12.5 and 25μg/ml). The apoptosis events were also detected in both types of cancer cell lines using mitochondrial membrane permeability, nuclear morphology, and genomic DNA fragmentation. The contribution of AgNPs to the levels of glutathione s-transferase was also determined. According to the results, AgNPs size was 28.43nm with spherical shape and peak UV-Vis absorbance ranged between 402-410nm. Concentration of AgNPs in ablated solution was 50μg/ml and used as stock concentration in cell lines experiments. Ablated silver nanoparticles was capable of inhibit the growth of both HeLa and SKOV-3 cancer cell lines in dose and time dependent manner, whereas it was less inhibitory against normal fibroblast cells. All three apoptosis detection method conducted indicated positive characteristics for apoptosis, while GSH levels did not varied significantly in both HeLa and SKOV-3 cells.
PVP solution, 1000 pluse was used with laser energy of 800 mJ/pulse. Formation of AgNPs-PVP was confirmed by UV visible spectrophotometer detecting surface plasmon resonance chang in the abilation solution. Concentration of AgNPs, size distribution, and surface charge of the ablated AgNPs in the produced solution was determined using atomic absorbtion spectroscopy and zeta potential analysis respectivly. Nanoparticles shape was determined using transmission electron microscope imaging. Ability of the senthezied AgNPs to inhibit the growth of cancer cells was compared to that of normal fibroblast cells using two cancer cell lines (HeLa and SKOV-3) in different concentrations (0.78, 1.56, 3.125, 6.25, 12.5 and 25μg/ml). The apoptosis events were also detected in both types of cancer cell lines using mitochondrial membrane permeability, nuclear morphology, and genomic DNA fragmentation. The contribution of AgNPs to the levels of glutathione s-transferase was also determined. According to the results, AgNPs size was 28.43nm with spherical shape and peak UV-Vis absorbance ranged between 402-410nm. Concentration of AgNPs in ablated solution was 50μg/ml and used as stock concentration in cell lines experiments. Ablated silver nanoparticles was capable of inhibit the growth of both HeLa and SKOV-3 cancer cell lines in dose and time dependent manner, whereas it was less inhibitory against normal fibroblast cells. All three apoptosis detection method conducted indicated positive characteristics for apoptosis, while GSH levels did not varied significantly in both HeLa and SKOV-3 cells.
Keywords
AgNPs
cancer cells
Cytotoxicity
DNA fragmentation
GSH
metal silver
MTP