Kufa Journal For Veterinary Medical Sciences

This study has been designed to analyse the secreted and soluble proteins in bovine seminal plasma by mass spectrometry. The majority of these proteins are produced by accessory glands, and partially by testis, epididymis, ductus deferens and vas deferens of male reproductive tract. Seminal plasma of bovine was collected freshly and isolated after centrifuged and removed the sperm. Non boiled and boiled seminal plasma lysate were run to identify and detect the total proteins, as well as the individual single member of ADAMs protein is determined. The non-boiled lysate of seminal plasma was displayed diversity of proteins more than boiled sample lysate of seminal plasma. However, boiled and non-boiled seminal plasma have distinguished several types of ADAMs protein which are included: ADAM10, ADAM9, ADAM7, ADAM15 in non-boiled samples, while boiled lysate of bovine seminal plasma was displayed ADAM10, ADAM9, ADAM28, and ADAM22. Our finding concluded that bovine seminal plasma is very rich in different types of soluble, cleaved and shed proteins such ADAMs protein which could potentially have a biological and physiological role in protection and interactions of sperm during motility inside female reproductive tract, as it might support it to fertilise the ovum. This protection might be via immunosuppression behaviour or by block specific receptors in female reproductive tract for enhancing sperm motility, and avoid sperm the singling response of immunity.

Cystic echinococcosis (CE) is a parasitic zoonotic disease which is highly endemic in Iraq and has an extensive effect on livestock productivity and human health. The current study focused on slaughtered sheep and cattle in Al-Najaf city abattoir, which infected with hydatid cysts from the period between January 2016 till December 2018. The total sheep examined were 244806, in which 3339 were found to be positive for hydatidosis, while in cattle, the total examined number was 59001, in which 887 were found to be positive for hydatidosis. In current study, the prevalence rate was estimated to be 1.49 % for hydatidosis in sheep, whereas in cattle it was 1.60 %. There were no differences between sheep and cattle rate infections. The highest rate of infection in sheep and cattle was observed in April 2.03% and July 1.89 respectively, while the lowest rate was observed in September 1.27% and November 1.04, respectively. Seasonally, the highest infection rate of hydatid cysts was 1.60 % in spring for sheep and 1.77 % in summer for cattle. The findings of this study demonstrated that the prevalence of hydatid cysts infections is generally lower than those reported from other regions of Iraq, and at the same time, it clearly remains prevalent. Hydatid cysts are common and widely distributed in sheep and cattle and they might play an important role in the life cycle and transmission of this zoonosis in Al-Najaf province.

This study conducted at central researches unit at the college of veterinary medicine in Basrah university and it aimed to extract silymarin components from local Milk thistle (Silybum marianum) that grow in north of Iraq(mosul) , at room temperature within different periods and 'isolation of silymarin oil from the plant, 50gram of grinded seeds defatted by normal hexane (500m1) using soxlet for 3 hr. and the defatted seeds powder extracted by absolute ethanol(1:3) at room temperature for 72 and 96 hr. After extraction of defatted grinded seeds of local silybum marianum with'absolute ethanol at room temperature. Results showed that at 96 hr.of extraction significantly effect the amount of extracted mat rial(24.3720+5.307mg/g p < 0.05) , while it was 10.9840+.92572 mg/g after72 hr. and the percentage of silymarin components were 1.109 +0.097and 2.435+ 0.0553% at 72 and 96hr. respectively and Silymarin oil that defatted from the grinded seeds yield 22.23%.

Authors describe the lower respiratory tract between sheep (Ovis aris) and goat(carpus hircus) by involved five adult male sheep and goats to investigate the anatomical features of lower respiratory tract in indigenous sheep and goat. The samples of the lower respiratory tract were collected from Baghdad slaughter house the external feature trachea and lung deals with the shape, weight, relationship, ligamentation and position of the lower respiratory tract. The trachea in both species was long flexible hollow tube with uncompleted cartilagenous rings were situated in the median line of cervical region ventrally, trachea was showed started from the first tracheal ring cartilaginous with the cricoids cartilage of the larynx. trachea was composed of two regions airway; cervical and thoracic, the tracheal ring shape were C shape in goat and U shape in sheep, the numbers of tracheal rings in sheep and goat were 49.2 ± 0.37, 51.2 ± 0.47, respectively. The total mean length of the right and left main bronchi in both sheep and goat were 2.26±0.092cm, 2.10±0.077cm, 2.12±0.037cm, 1.96±0.005cm, respectively. The total mean tracheal length in both sheep and goat were 30.4± 0.50cm, 24.7±0.34cm, respectively. The lungs of both animals look like spongy soft organ, surrounded the heart which was located in the mediastinum and enveloped by visceral and parietal pleura. The lung of both animals have anterior apex and posterior base, lung composed right and left lobe the right apical lobe subdivided into cranial and caudal part while undivided in goat, both animals left lung composed of apical and caudal lobe, the apical was subdivided into cranial and caudal part

Background:Because of the fact that the mechanism of anticonvulsant action of Gabapentin is not yet clear, so the aim of our present study was to determine whether the voltage-gated sodium channels (VGSC) may be correlate with its mechanism. To achieve this goal, Cypermethrin was chosen as a (convulsion inducer) resulting from its prolongs the opening of (VGSC) in order to interfere with Gabapentin . Method:The experiment animals were divided into four groups. The first group was treated with a single dose of Cypermethrin (1000 mg / kg, orally), while the second and third group were treated with a single dose of Gabapentin (100 mg / kg, orally)15 or 30 minutes before the Cypermethrin treatment respectively. The fourth group was treated with Gabapentin alone in a dose of (100 mg / kg, orally) .After the end of treatment of the chicks were transferred to the cages to be monitored individually and recorded percentages of appearance of nervous signs and the percentage of each mortality and protection against mortality. Results: Chicks treated with Cypermethrin alone (1000 mg / kg, orally)showed nervous signs which included the jerking movements of the leg and wings (clonic convulsion) and whole body tremors accompanied with opisthotonos at percentages (80%, 100%,60%) respectively that end with death at (100%) , but the pretreatment of chicks with Gabapentin (100 mg / kg, orally) resulted in time-dependent protection against Cypermethrin-induced nervous signs and mortality,which representing by significantly decrease in the percentage of each clonic convulsion,whole body tremors and mortality to (0%, 20% and 20%) respectively and accompanied by significant increase the percentage of protection of chicks against mortality to (80%) in the group that pretreatment with Gabapentin 30 minute before administration of Cypermethrin compared with control group (Cypermethrin alone). We conclude from our results that recorded for the first time the Gabapentin provided protection to chicks against Cypermethrin-induced nervous signs and mortality , this result may be related to effect of Gabapentin on (VGSC). Thus, the currently study will open the way to use Cypermethrin as a model in scientific research that detect the mechanisms of action of anticonvulsant drugs .

The study conducted to investigate of the Diarrheagenic Escherichia coli (DEC) microbial load in which contaminate the locally produce cheese from milk of local Awassi breed ewes . And to indicate the effect of different types and concentrations of mixture’s of Emulsifying salts to choose the best mix of them that reduce the Diarrheagenic Escherichia coli (DEC) microbial load in this locally produce cheese . A 60 sample Cheese locally produced from milk of Awassi ewes were been collected randomly from Local Awassi Flock of College of Agriculture at Baghdad University (Iraq) , ( 30 samples to each winter and spring season ) to investigate their microbial load . Both of all winter and spring samples were in high significant (p<0.01) microbial count . The Bacteristatic & Bactericidal effect of Emulsifying salts on microbial activity was confirmed when each of the Total Bacterial Count (TBC), Total Coli form Count, Total Escherichia coli Count and Total Diarrheagenic Escherichia coli (DEC) Count were highly significant (p<0.01) reduced in the cooked Cheese Samples with 3% Emulsifying salts composed of ( 90% (Na5P3O10) + 10% (Na3C6H5O7) ) were being added

Background and aims: This experiment was conducted in order to compare the effect of Platelet-rich plasma (PRP) and low-level laser (LLL) therapy on healing of second-degree burn wound in sheep. Materials and Methods: Fourteen adult rams were divided into four groups of equal numbers. A second-degree burn was generated on the back of all animals. The burned area in the first and second group was exposed and autologous PRP and LLL (6 J/cm2), respectively were applied on burned area continuously for five days. Fourteen days later, samples were collected from all animals for histopathological examinations. Results: Histopathological evaluations on the second week showed the burn healing to be better in the PRP than LLL and both of them were better with respect to the control group. Conclusion: The application of PRP for five days is effective than LLL application in healing burn-related skin wounds in sheep model.

The present study aimed to investigated Giardia duodenalis in cattle in some different areas of Wasit province by using molecular study and verification of the genotype of Giardia duodenalis. Collected one hundred fecal samples from cattle, the result showed that the rate of infection was 83% (100) . DNA was extracted from the 100 positive samples from the cattle then amplified using the special tris-phosphatesomerase gene for genotyping A and B. The result of type A infection was (69%) and (45%) of the genotype B. The purpose of this study was to investigate the genotypes of cattle in Wasit province and compare them with previous sources at the NCBI data bank.

The current study includes eye balls of Iraqi geese (anser anser). The anatomical characteristics and histological eye structure. The eye ball shape was nearly spherical. The general mean size of the geese's right and left eye was 2.3 ± 0.07 ml. The ciliary body was the anterior part of the choroid and it joined anteriorly with the iris. The retina was the deepest layer of the eyeball jacket. The retina had two parts, one sensory and the other meaningless. The sensory part of the retina of the ciliary body began to cover the ciliary body and the iris. The sensory part of the retina consists of ten layers: (1) the pigmented epithelium of the retina (2) of the rods and cones (3) the outer specific membrane (4) the outer nuclear layer (5) the outer layer (6) Internal plexus (8) Layer node cell (9) Fiber optic layer (10) Internal boundary membrane. . The total values of retinal thickness is 304.68±0.95 μm.