Abstract
A simple, rapid, and accurate spectrophotometric method is suggested for the estimation of phenylephrine -HCl (PHE-H). The method included adding an excess amount (known) of N-bromosuccinamide (NBS) in an acidic medium to oxidize PHE-E, and then the residual NBS was estimated by the bleaching color of methyl orange dye(M.O), the intensity of bleaching dye at the 518 nm was measured. The NBS amount reacts to PHE-H concentration in the actual solution. Beer's law was obeyed in the 2-25 μg /20 ml range with good molar absorptivity of 1.0705x 105 l.mol-1.cm-1 The proposed method was applied successfully to determine PHE-H in its pharmaceutical preparation.
Keywords
bleaching color
methyl orange
N-bromosuccinamide
phenylephrine–HCl
spectrophotometric determination