Abstract
Glioblastoma is one of the most prevalent brain tumors
and was the leading cause of cancer death. Since this
cancer is an aggressive, incredibly invasive, and
neurologically debilitating tumor that does not respond
well to chemotherapy, a group of researchers looked at
alternative, more efficient therapies. The current study
focused on using biological agents and their products
(Lavender essential oil and Newcastle disease
virus(NDV)) to test their toxicity against the human
cerebral glioblastoma (AMGM-5) as a cancer cell model
by 3-(4, 5- dimethylthiazol-2-yl-2-) 2.5-
diphenyltetrazolium bromide (MTT) viability assay. In
brief, the cells were exposed to several concentrations of
lavender oil and serial multiplicity of infection (MOI) of
NDV for 72 hours. The result shows significantly
decreased cell viability treated with lavender oil and
NDV in a concentration-dependent manner. The
inhibitory concentration that kills 50% of the cells (IC50)
of lavender oil was 4.471µg/ml, and the IC50 value of
NDV was 0.00473MOI. Also, the morphological study
was conducted by the traditional method by using
hematoxylin & eosin (H&E) and apoptotic assay by using
Acridine orange/Ethidium bromide (AO/EB), the changes
are apoptosis and degeneration, as well as cell the
emergence of necrotic cells. The compounds of lavender
oil were probably responsible for the cytotoxicity of
lavender oil. Our results revealed the antagonistic effect
of combined essential oil and NDV. Essential oil and
NDV have the same toxicity potential for glioblastoma.
and was the leading cause of cancer death. Since this
cancer is an aggressive, incredibly invasive, and
neurologically debilitating tumor that does not respond
well to chemotherapy, a group of researchers looked at
alternative, more efficient therapies. The current study
focused on using biological agents and their products
(Lavender essential oil and Newcastle disease
virus(NDV)) to test their toxicity against the human
cerebral glioblastoma (AMGM-5) as a cancer cell model
by 3-(4, 5- dimethylthiazol-2-yl-2-) 2.5-
diphenyltetrazolium bromide (MTT) viability assay. In
brief, the cells were exposed to several concentrations of
lavender oil and serial multiplicity of infection (MOI) of
NDV for 72 hours. The result shows significantly
decreased cell viability treated with lavender oil and
NDV in a concentration-dependent manner. The
inhibitory concentration that kills 50% of the cells (IC50)
of lavender oil was 4.471µg/ml, and the IC50 value of
NDV was 0.00473MOI. Also, the morphological study
was conducted by the traditional method by using
hematoxylin & eosin (H&E) and apoptotic assay by using
Acridine orange/Ethidium bromide (AO/EB), the changes
are apoptosis and degeneration, as well as cell the
emergence of necrotic cells. The compounds of lavender
oil were probably responsible for the cytotoxicity of
lavender oil. Our results revealed the antagonistic effect
of combined essential oil and NDV. Essential oil and
NDV have the same toxicity potential for glioblastoma.
Keywords
Anti-cancer activity
cell lines
Lavender oil
NDV
Abstract
يعد ورم الأرومة الدبقية أحد أكثر أورام الدماغ انتشارًا و السبب الرئيسي للوفيات الناتجة من السرطان. ونظرًا لأن هذا النوع من الاورام عدواني وغاز بشكل لا يصدق وموهن عصبيًا ولا يستجيب جيدًا للعلاج الكيميائي ، فقد اتجهت انظار الباحثين نحو ايجاد علاجات بديلة أكثر كفاءة. ركزت الدراسة الحالية على استخدام العوامل البيولوجية ومنتجاتها (زيت اللافندر وفيروس مرض نيوكاسل) لاختبار سميتها ضد ورم الأرومة الدبقية البشري (AMGM-5) كنموذج للخلايا السرطانية باستعمال اختبار (3-(4, 5- dimethylthiazol-2-yl-2-) 2.5- diphenyltetrazolium bromide (MTT)). باختصار ، تم تعريض الخلايا لتراكيز متعددة من زيت اللافندر والتعدد المتسلسل للعدوى (MOI) من فيروس مرض نيوكاسل لمدة 72 ساعة. تظهر النتيجة انخفاضًا كبيرًا في حيوية الخلايا المعالجة بزيت اللافندر و NDV بطريقة تعتمد على التركيز. كان التركيز المثبط الذي يقتل 50٪ من الخلايا (IC50) من زيت اللافندر 4.471 ميكروغرام / مل ، بينما كانت قيمة IC50 لفيروس مرض نيوكاسل 0.00473MOI . أجريت الدراسة المورفولوجية بالطريقة التقليدية باستخدام (hematoxylin & eosin, H&E) ودراسة موت الخلايا المبرمج باستخدام (acridin orang/ ethidium bromide, AO / EB) ، تراوحت التغيرات ما بين موت الخلايا المبرمج والتنكس ، وكذلك ظهور الخلايا الميتة. تعود السمية الخلوية لزيت اللافندر للمركبات الكيميائية التي يحتويها. كشفت نتائجنا عن التأثير المضاد لزيت اللافندر و NDV. أيضا كشفت الدراسة ان زيت اللافندر و فيروس مرض نيوكاسل لهما نفس احتمالية السمية للورم الأرومي الدبقي.
Keywords
الخطوط الخلوية
النشاط المضاد للسرطان
زيت اللافندر
فيروس مرض نيوكاسل