Abstract
A rapid, efficient and reproducible propagation protocol was developed for initiation,
multiplication and root formation of poinsettia (Euphorbia pulcherrima Willd.) through the culture of
single nodal segments on basal MS medium. The results showed that the best cultures were established on
solid MS medium supplemented with (5.0+0.1) from both BA and NAA respectively after being surface
disinfested in %07 ethyl alcohol for 00 seconds, followed by immersion in mercuric chloride (0.57) for
five minutes. The best shoots multiplication was achieved with the combination treatment 5.0+5.0 mg/l
of BA and NAA (6.16 shoots/ explant) as compared with the use of BA or Kinetin separately. The
rooting percent (5007) was recorded by adding 0.0 and 0.0 mg/l IBA. IBA performance was better than
IAA in producing higher number of roots by giving 6.60 roots/ explant on shoots cultured on solid MS
medium supplemented with 0.0 mg/l IBA. A high survival rate (%07) was found with poinsettia plantlets
gradually acclimatized into the out-air conditions.
multiplication and root formation of poinsettia (Euphorbia pulcherrima Willd.) through the culture of
single nodal segments on basal MS medium. The results showed that the best cultures were established on
solid MS medium supplemented with (5.0+0.1) from both BA and NAA respectively after being surface
disinfested in %07 ethyl alcohol for 00 seconds, followed by immersion in mercuric chloride (0.57) for
five minutes. The best shoots multiplication was achieved with the combination treatment 5.0+5.0 mg/l
of BA and NAA (6.16 shoots/ explant) as compared with the use of BA or Kinetin separately. The
rooting percent (5007) was recorded by adding 0.0 and 0.0 mg/l IBA. IBA performance was better than
IAA in producing higher number of roots by giving 6.60 roots/ explant on shoots cultured on solid MS
medium supplemented with 0.0 mg/l IBA. A high survival rate (%07) was found with poinsettia plantlets
gradually acclimatized into the out-air conditions.
Keywords
Euphorbia pulcherrima Willd.
in vitro
Micropropagation
Poinsettia