Abstract
One of the most significant helminthozoonosis in Iraq and
around the world is cystic echinococcosis, which occur by the
etiologic agent of Echinococcus granulosus and it’s
distinguished by considerable intra-specific variability
(genotypes G1–G10). DNA was extracted from 48 isolated
cysts (25 sheep, 12 goats and 11 human) and used as
templates to amplify using Trachsel method to differentiate
Taeniide (Echinococcus spp. and/ or Taenia spp.) and
mitochondrial cytochrome c oxidase subunit one (COX1)
gene for genotyping. The PCR products were sequenced, and
sequence analysis was used to further evaluate the data. Out
of 48 separated of the host cysts, 36 isolates displayed the G1
genotype (100%), and in 12 samples, a nucleotide substitution
at position 58 (T→C) results in polymorphism (99.9%). The
G1 is considered that the most contagious and prevalent
genotype of E. granulosus in the world. Our investigation
revealed that, a single genotype that may be in charge of the
disease's infectivity in sheep, goats, and human as well as its
persistence in endemic areas. these epidemiological findings
could be guided the successful hydatidosis con
around the world is cystic echinococcosis, which occur by the
etiologic agent of Echinococcus granulosus and it’s
distinguished by considerable intra-specific variability
(genotypes G1–G10). DNA was extracted from 48 isolated
cysts (25 sheep, 12 goats and 11 human) and used as
templates to amplify using Trachsel method to differentiate
Taeniide (Echinococcus spp. and/ or Taenia spp.) and
mitochondrial cytochrome c oxidase subunit one (COX1)
gene for genotyping. The PCR products were sequenced, and
sequence analysis was used to further evaluate the data. Out
of 48 separated of the host cysts, 36 isolates displayed the G1
genotype (100%), and in 12 samples, a nucleotide substitution
at position 58 (T→C) results in polymorphism (99.9%). The
G1 is considered that the most contagious and prevalent
genotype of E. granulosus in the world. Our investigation
revealed that, a single genotype that may be in charge of the
disease's infectivity in sheep, goats, and human as well as its
persistence in endemic areas. these epidemiological findings
could be guided the successful hydatidosis con
Keywords
Multiplex PCR-Diagnosis in intermediate host;
Abstract
أحد أهم أمراض الديدان الطفيلية في العراق وحول العالم هو داء المشوكات الكيسي، والذي يحدث بواسطة العامل المسبب لمرض المشوكة الحبيبية ويتميز بتباين كبير داخل النوع (الأنماط الجينية G1 – G10). تم استخراج الحمض النووي من 48 كيسًا معزولًا (25 خروفًا و12 ماعزًا و11
Keywords
أحد أهؼ أمراض الديدان الظفيمية في العراق وحؾل