Abstract
Penicillium expansum produced the toxin patulin in pome fruits. To evaluate the
molecular mechanism by which the treatment of probiotic bacteria Bifidobacterium breve and
Lactobacillus salivarius could modulate patulin production, three genes involved in the
biosynthesis of patulin were measured using real time-PCR technique. The result of this study
found that the supplementation with B. brave and L. salivarius down-regulate the relative
expression of 6-methylsalicylic acid synthase (msas), ATP binding cassette transporter (ABC)
and putative cytochrome P450 monooxygenases (P450-1) as well. Although, there is no effect
of some strains on this expression. However, these finding suggested that these bacteria
decreased patulin production through effect on the genes responsible for its biosynthesis
which can be the main mechanism by which patulin reduced in the presence of these bacteria.
molecular mechanism by which the treatment of probiotic bacteria Bifidobacterium breve and
Lactobacillus salivarius could modulate patulin production, three genes involved in the
biosynthesis of patulin were measured using real time-PCR technique. The result of this study
found that the supplementation with B. brave and L. salivarius down-regulate the relative
expression of 6-methylsalicylic acid synthase (msas), ATP binding cassette transporter (ABC)
and putative cytochrome P450 monooxygenases (P450-1) as well. Although, there is no effect
of some strains on this expression. However, these finding suggested that these bacteria
decreased patulin production through effect on the genes responsible for its biosynthesis
which can be the main mechanism by which patulin reduced in the presence of these bacteria.
Keywords
Lactic acid Bacteria
Patulin
Penicillium expansum and gene