Basrah Journal of Veterinary Research

In order to increase egg and chicken meat production, poultry growth is critical. The purpose of this study is to see how well broiler chickens perform when provided different additives in their meals (trace minerals and vitamins). Nutraceuticals, in addition to providing nutritional requirements for birds, offer advantageous pharmacological effects, such as assisting in the establishment of normal state of physiological health, preventing various diseases, and thus improving production performance. The vitamins, polypeptides and amino acids, minerals, enzymes, and other nutraceuticals are examples. Vitamins are dietary substances that are required for vital cell functions such as cell development, growth, and metabolism process. Mineral nutrition is critical in broiler chicken production, with an emphasis on manganese (Mn), zinc (Zn), selenium (Se) and copper (Cu). Due to the availability of organic trace elements and today's fast-growing birds, broiler chickens' Mn, Zn, Se, and Cu requirements must be revised.

The goal of the present study was to isolate and identify (CRISPR-Cas) elements in S. epidermidis (MRSE) from 300 samples collected from various sources. Culture techniques, biochemical tests and VITEK2 system were used for confirming of Staphylococcus epidermidis isolation. The partial sequencing of the 16s rRNA gene was evaluated and compared with those in the Gene Bank to find differences in the sequence using the BLAST tool (http://www.ncbi.nlm.nih.gov). On the other hand, the CRISPR-Cas system was examined in all isolates of multidrug-resistant S. epidermidis in which the SECR1 and SECR2 elements were not found. The cas6 element was found in all of the tested bacterial strains. Susceptibility tests were done using the disc diffusion method after identification. Antibiotic resistance testing on S. epidermidis isolates revealed the highest percentage of multi-resistance to ampicillin, ceftriaxone, chloramphenicol, cefalexin, vancomycin, nalidixic acid at 100%. Moreover, S. epidermidis isolates showed intermediate effect against amoxillin-clavulanic acid, tobramycin, neomycin, oxacillin, tetracycline and methicillin. However, S. epidermidis isolates were found to be the most sensitive to erythromycin, norfloxacin and rifampicin. The CRISPR-Cas system can be found in S. epidermidis isolates from a variety of local sources and that the appearance of the CRISPR system in S. epidermidis isolates plays a unique role in antibiotic suitability.

Plastination is an anatomical preservation process Samples by precise technique of compulsory impregnation by processable polymer such as silicone, epoxy or polyester Resins that have wide usages in the medical field. The tissue reservation technology was developed by Dr. Gunther von Hagens in 1978. By this method we can store and preserve the body and wipes it for the aim of learning. Current there are new and various kinds of plastination used the learning and in museums. This review aims to explain the main steps of soft plastination used either techniques or methods and their implementation for the medical and veterinary educations.

Diabetes mellitus (DM) is a metabolic disease involving improperly high blood glucose level, hyperlipidemia and hypoinsulinemia. The aim of current study is to assess and compare the influence of Quercus infectoria galls (QIg) extract and glimepiride on pancreatic β-cells secretory function and serum lipid profile in diabetic rats. In the present study, thirty six male rats were used and divided into six groups (n=6), including negative control, STZ induced diabetic control, treated diabetic rats with glimepiride (200mg/Kg Bw), and treated diabetic rats with different concentrations of QIg extract, 200 mg, 400 mg and 800 mg/Kg Bw for 42 days, respectively. At the end of the experiment, all rats were fasted overnight, blood samples were collected for measuring fasting blood glucose, insulin hormone and lipid profile. All rats were then scarified by using chloroform in order to take part of the pancreatic tissue. The results showed a significant decrease in blood glucose, total cholesterol, triglycerides, low density lipoprotein, very low-density lipoprotein, HbA1c% with obvious elevation in serum insulin hormone and high-density lipoprotein in diabetic rats treated with QIg extract and glimepiride compared with diabetic untreated group. In addition, high atherogenic index in diabetic rats was significantly reduced by different concentrations of QIg extract and glimepiride. Histopathological results revealed that there was a significant and dose-dependent morphological restoration in the pancreatic structure damages especially at high dose of the extract. These findings provide a new insight into the role of QIg methanolic extract in maintaining normal blood glucose and adequate pancreatic insulin hormone secretion in diabetic rats, amelioration of dyslipidemia and associated cardiovascular alterations with subsequent improvement in pancreatic structural damages in diabetic rats.

The current study aimed at the isolation and identification of some fungal types that infected the lungs and rumens of small ruminants (non-healthy animals). To achieve this goal, 100 samples from sheep, and goat lungs and rumen tissues (50 for each), were collected from the Basrah slaughterhouse. Fungi were isolated from the samples and diagnosed based on the morphology of colonies cultured on Sabraud Dextrose Agar (SDA) media, then detected microscopically after staining with methylene cotton blue and Indian ink stain. PCR and DNA sequencing of the18S rRNA ribosomal gene confirmed the important and common fungal isolates. The current study showed the presence of fungal isolates in lung samples of sheep including, Cryptococcus spp. (29.43%) represented the highest percentage. While the lowest percentage was Coccidioides spp. accounted for (4.878%). However, those isolated from the rumen of sheep in high percent was Cryptococcus spp. (48.571%), and finally, the lungs contained Coccidioides spp. (4.878%). The result of fungal isolates from the lungs of the goats in high numbers was Aspergillus spp. (42.5%), the fungal isolate that appears in low numbers, Candida spp. (7.5%).