Journal of Biotechnology Research Center

Analysis of touched DNA from crime scenes is fundamental in forensic DNA laboratories. Many factors affect the recovery of DNA from touched surfaces and then affect the quality of the final results. The aim of this work is studying the possibility of recovery suitable amount of DNA from ltouched portable computer. The computer was cleaned with 10% Bleach then touch and DNA collected for extraction by Organic method and two STR regions D5S818 (115-163bp) and FGA (308-464bp) were amplified. The results showed that it is possible to isolate a proper amount of DNA from touched portable computer where it was amplified and then analyzed by Agarose gel electrophoresis. The conclusion is that portable computer is suitable source for forensic analysis.

Medicines derivative from plants broadly famous owing to their safety, obtainability and little Leaves, roots, bark, kernels and florae of plant are mostly prepared that included herbal medicines. They are administered orally, inhaled or directly applied in the skin. Ruta chalepensis is take on in the outdated medicine of many countries as a natural herb of the Mediterranean region. Pharmacological properties responsible by using phytochemical screening has shown the attendance of bioactive molecules price. The current study meant to investigate the effect of R. chalepensis ethanolic extract on liver function enzyme (Aspartate Amino-Transferase (AST), Alanine Amino-Transferase (ALT) and Alkaline Phosphatase (ALP) and histological examination of liver section. Obtained showed the ability of plant extract to protect liver from any xenobiotics and provided protection against CCL4 damage on albino male mice.

Food is fundamental for the survival of all living forms. Sometimes, food gets contaminated by different types of microbes, such as bacteria, viruses,protozoa and helminthes, at any stage of food chain. Foodborne pathogens can cause life threatening infections especially in children, elderly immune compromised individuals, and pregnant women. In current years, many viruses have been defined known as main sources of foodborne diseases. Among these, Hepatitis A, HepatitisE, Rotaviruses etc.The aim of the study was to investigate on common food borne viruses, foods that transmitted them, symptoms that appear on infected people & protection methods.
Viruses are transmitted via food, water,inanimate objects and person-to-person contact.The significance of foodborne viruses is progressively more documented as causes of infection in humans. People usually acquire infection orally, after swallowing of polluted foods throughout processing, handling or preparation. Each type of foodborne viruses initiated from the personal gut, and pollution of food happens either by an infected food handler throughout preparation or by contact with sewage or contaminated water. Numerous outbreakes of foodborne viral disease are related with the pollution of ready for consumption foods via the infection of food handlers.

Orobanche aegyptiaca Pers., Orobanche cernua L. and Orobanche coelestis (Reut.) Boiss. and Reut. ex Beck are plants belong to the Orobanchaceae family in Iraq. Morphological and anatomical study of the three species is very important to to know the qualities that help us to distinguish between them. To study the morphological and anatomical traits the samples were collected from Baghdad, the southern deserts, and Kurdistan of Iraq between 2016-2018 during the flowering period (spring). The plant was preserved in special glass ampoules containing alcohol 70% for the purpose of conducting the transvere anatomy section process. The identity and morphological study as well as the anatomical study of the three species were determined by studying the anatomical transverse sections of the stem and studying the trichomes which are generally divided into two parts: Non-glandular trichomes which consist of from one or more cells, different lengths with acute or obtuse apices, and glandular trichomes which consist of a multicellular or unicellular head and stalks depended on their shape, number of cells and general characteristics as a method or Identity imprint of the plant used to differentiate among the studied species. Significant differences were observed in the general and anatomical appearance. The species Orobanche aegyptiaca Pers. is more dense in the amount of trichomes spread in all parts of the plant compared to other, O. aegyptiaca Pers. be containing 18–22 layers of parenchyma cells, the species O. cernua L. consists of 10-16 layer of parenchyma cells and the species O. coelestis (Reut.) Boiss. And Reut.exBeck consists of 20-24 layers of parenchyma cells. Morphological and anatomical study have proven its effectiveness in determining mportant characteristics in diagnosing species and distinguishing between them.

Following suicide bombing, human remains usually collected for DNA identification. Human remains affected due to exposure to excessive heat and bomb solid, sharp pieces. The aim of this work is to study the possibility of DNA analyzing of human remains after suicide bombing. Fifteen human remains were received from a place of suicide bombing in Najaf City south of Iraq. The remains were severely affected and cannot recognize morphologically or as it is human or animal origin. DNA extracted from remains by organic method, then quantified by real time PCR kit then analyzed by Powerplex 21 kit using 3130XL Genetic Analyzer. The results showed that seven remains where analyzed successfully while the other remains failed for analysis for both real time PCR quantification or STR analysis. Four remains belong to two persons. This study showed that suicide bombing affects negatively the most remains for STR analysis.

Civilian patients with severe burns were almost local and systemic cell host immune responses concurrent depression because of complications after microbial infections. The most abundant and harmful bacterial infections are associated with opportunistic bacteria Staphylococcus aureus and Pseudomonas aeruginosa, linked to nosocomial infection with prolonged stay in a hospital. Many virulence factors excrete from both species that facilitate adherence to the burned area, localized and invasion tissue, and the emergence of antibacterial resistance. Molecular docking of molecules assimilates the interaction between a ligand and its receptor the virulence factor; this project designed to show the extent of interaction between active component extracted from black tea and mint and virulence factors (coagulase, exozyme S, the extended-spectrum of beta- lactamase and beta-lactam binding protein) using molecular docking. Aqueous extracts of black tea and mint were analyzed by GC mass and each of the bioactive components was docked against each virulence factor. Re- rank score was monitored to explain the power of interaction between ligand and receptor.Results found here that components of black tea gave more re-rank score with all virulence factors; the most active was Myricetin that needs -80.906, -80.19, -69.04, and -67.06 for PBP2 protein, exozyne S, extended-spectrum beta-lactamase, and coagulase respectively. It was noticed that Myricetin was more active against proteins excreted from P. aeruginosa than that of S. aureus. Kaempferol as a second bioactive compound that gave a positive effect regarding its re rank score -90.195 and -79.54 when docked with PBP2 and exozyme S from P.aeruginosa.Molecular docking revealed that the aqueous extract of both black tea and mint have high re-rank as inhibitory substances against different virulence factors of S.aureus and P.aeruginosa that could be a good candidate for treating burns injuries.

Traditionally medicinal plants are used for its potential chemotherapeutic action and for safety upon the continued use of these plants. Many medicinal plants are containing great bioactive components that reported to preventive and therapeutic role on different body organs. Violet (Viola odorata) is a medicinal plant that had different groups of compounds like cyclotides, flavonoids, alkaloids, triterpenoids, violin, quercitin, steroids, tannins, and saponins) which possessed different biological activity such as antifungal, antibacterial, anticancer, antioxidant, antiasthmatic. This study focused the line on the effect of different concentration of Viola odorata extract on blood cholesterol, triglyceride, total protein, albumin, creatinin, and immunological effect (IgM, IgG, IgA) in serum of white albino male mice as well as, toxic effects of methotrexate drug. the results indicated that Viola odorata had the ability to counteract the toxic effect on all parameters studied due to presenting of flavonoid compound (isorhamnetin and luteolin) belong to flavonols class which is well reported to have hepatoprotective activity and had high detoxification effect against MTX drug. Also, a substantial effect which explained the ability of plant extracts to interfere with the MTX and prevented its harmful effect. The plant is safe to be used as an effective remedy against different xenobiotic.