Basrah Journal of Veterinary Research

To study the effect of over dosages of dexamethasone on eye rabbits; a total of 20
healthy adult rabbits were used. Rabbits were equally divided in to 4 groups, the
rabbit in each group wastreated with dexamethasone intramuscular twice daily for 30
days. The dexamethasone doses were10, 20, 30, and 40 mg/kg/day, for group 1, 2, 3,
and 4 respectively. After that the animals were sacrificed, histological sections of
cornea samples were prepared using hematoxylin and eosin staining. The entire
experiment was completed in the animal facility at Veterinary Medicine College,
University of Basrah. Rabbits in Group 1 and 2 remain healthy and there are no side
effects of dexamethasone while group 3 and 4 the side effects of dexamethasone were
cataract and emaciation. The cataract was clear clinically and histologically. The
development of dexamethasone-induced cataract need much work to elucidate the
mechanism

The study were conducted on 31 local female buffaloes breeds ,In basrah –Iraq ,
during the period from 2010 – 2013 , 6 – 15 years old, among these 21 local female
buffaloes breeds show signs of recurrent tympany one to two months after parturition,
and 10 clinically healthy local female buffaloes breeds were served as controls ,
moreover living status and kind of forage was archived before and after parturition ,
nevertheless almost cases were not respond to the classical treatment when it given by the
owners. Results of ruminal histopathological examination after rumenatomy indicated
rumenitis , ruminal adhesions and keratosis of rumeinal wall . It have been concluded that
changing to holly concentrated food in late stage of the pregnancy might considered as
the important cause of frothy tympany.

The present study was carried out at the animal house of the College of Veterinary
Medicine, University of Basrah. This study has been designed to determine whether
isoflovonoid extract of Punica granatum rinds effect on fertility efficiency and semen
fluid characteristic (physical and biochemical properties). Twenty four adult rabbits,
six-month-old and 1500-1750 body weight divided into two groups12 rabbits( 6 male
and 6 female) each group (male used for treated with extract while female only used
for fertility test (untreated with extract). First group: male rabbits received orally
administration of normal saline (3ml) served as control. Second group: male rabbits
received orally administration of isoflavonoid extract of Punica granatum rinds at the
dose of 0.5g/kg B.W dissolve in (3ml) of normal saline. Treatment with isoflovonoid
extract of Punica granatum rinds 0.5g/kg B.W. caused significant (p< 0.05) increases
in fertility efficiency such as number of offspring, sperm count, activities of sperm
viability, number of sperm live to dead, motility, zinc and testosterone concentrations
of treated rabbits in serum and semen plasma compared to control. Isoflavonoid
extract of Punica granatum rind caused no significant changes in biochemical
analysis such as total protein, ALP, ACP, AST and ALT in serum while ALP, ACP,
AST and ALT concentrations significant (P<0.05) decrease in semen plasma.
Treatment with isoflovonoid extract of Punica granatum rinds caused significant
(p<0.05) decreases glucose level in serum and abnormal sperm morphology. It caused
no visible lesion in the seminiferous tubules and spermatogenesis.
These findings revealed that isoflovonoid extract could cause ameliorating effect on
the reproductive parameters in male rabbits.

A total of 400 samples ( 200 blood samples and 200 vaginal swabs ) were collected
from cows throughout this study in Al-Basrah province . Clinical specimens should be
inoculated on to primary isolation media, like Sabouraud dextrose agar (SDA) ,then the
study showed that 90 out of 200 vaginal swabs (45%) were positive for the presence of
Candida isolates while 100% of the blood samples were negative for Candida. All the
obtained yeast isolates were subjected to identification by using germ tube test and
chlamydo spore,the results showed that 72.22% and 38.88 of yeast were able to produce
germ tube and chlamydo spores respectively ,. To differentiate between Candida species,
CHROMAgar™ Candida Agar was used. The results showed that 38.88 % of isolates
were identified as C.albicans, 28.88 % were C.tropicallis, 24.44% were C.krusei and
7.77% were identified as others species. Biochemical tests were done using API 20 AUX
strips. By this test the results confirmed that 80% of isolates were C.albicans, 8 % were
C. tropicallis , 4 % were C.glabrata , 4% were C.parpsilosis and 4 % were C.famata.
Phenotyping tests showed that 85 % of C. albicanscould grew on SDA agar with 6.5 %
NaCl, and all strains (100 % ) were grown at 45˚ C , while 75 % were grown on SDA
medium with cycloheximide .By using disc diffusion method for 7 antifungal Agents
against 20 C.albicans isolates. They were Fluconazole, Clotrimazole, Miconazole and
Ketoconazole showed the highest sensitivity to C.albicans with a percent of (90%)
,(80%), (80%) and (70%) ,revealed lower susceptibility. Further molecular identification
using multiplex polymerase chain reaction (PCR) was done for detection of some
virulence genes in the previously identify C.albicans.The result showed that 30 % of the
isolates having INT1 gene

In order to evaluate the toxic pathological effect of sodium dichromate, a (30) rats
were exposed to sodium dichromate in daily oral dose, these rats divided into 3 groups
based on two doses T1 group (3mg/kg bw) and T2 group (9mg/kg bw) in addition to
control group C were given distal water orally at the same time of experiment, The
animals were sacrificed after (30 and 60) days of administration, then detected the
toxicological effects of sodium dichromate by using body weight gain and
pathological changes, the results showed significant decrease in body weight of T2
than T1 and control groups respectively. In addition, the pathological changes appear
more sever at T2 than T1 compared with control group