Al-Kufa University Journal for Biology

تعتبر خميرة Saccharomyces cerevisiae من أهم المعززات الحيوية التي تمتاز بامتلاكها عدة أرقام متنوعة لتنشيطها الحيوية،
مما يعطيها قوة علاجية ووقائية في الوقت نفسه. ويهدف هذا البحث إلى دورية الفراولة المثبطة المنتجة من هذه الخيمائر في
النموذج العلمي للبكتريا المرضية . وبالتالي فقد جمعت 30 عزلة من بكتريا المكورات العنقودية الذهبية المعزولة منها
تم الحصول على عينة مرضية مختلفة من بعض مختبرات مستشفيات بغداد، وتم تقصي الفحص الفني للمنتج من قبل S. aureus
باستعمال طريقة اطباق التجديد الصغيرة طريقة ألواح المعايرة الدقيقة (MTP) . اذ تبقى نسبة العزلات كاملة لنشوء كيمياء
56.7% والضعفاء 26.6%، في حين كانت نسب العزل غير المسموح بها 16.7%. استعملت خميرة الخبز الجاهزة
( Packmaya ) Saccharomyces cerevisiae التركية المنشأ والمتوافرة في الاسواق المحلية . يساعد على الترطيب باستعمال المثبط
الترسيب سلفات الامونيوم بنسب اشباع تظهر ما بين 30 الى 70% من فرسان الثور في وسط النمو للعزلة S. cerevisiae PY ، تلاها إجراءات التنافذ الغشائي الى تفعيل نتيجة 0.511 ملغم / ملي بروتين ، وكان بروتين المثبط والمنشط جزئيا يؤثر بشكل مضاد لتكوين
ومع تزايد حرارة بكتريا S.aureus، بلغ البروتين المثبط للادنى للمنقى جزئيًا (64) ملجرام/ملليتر نحو معظم المعزولات.
وتمييز البروتين البروتيني البرونزي والمنقى جزئيا لتكوين شعيرية لمكافحة عزلات بكتريا S.aureus , وعزلة الاكثر تحسسا
هي المعزولة من الانف، فيما كانت البكتريا المعزولة من الجروح الأقل تحسساً له.

The study included collecting 100 vaginal secretion swabs from pregnant and non-pregnant women, aborted and non-aborted women, aged between (16-48) years, who visited Al-Zahraa (PBUH) Teaching Hospital and outpatient clinics in the city of Najaf during the period from November 2012 to March 2013. The results of the AMSL tests showed the prevalence of bacterial vaginosis among pregnant and non-pregnant women, aborted and non-aborted women, and the highest rate of bacterial vaginosis was among pregnant women who had aborted more than once and whose ages ranged between (16-22) years, reaching 43%, while the lowest rate was among non-pregnant and non-aborted women whose ages ranged between (37-48) years, reaching 11%. The results of isolation, laboratory diagnosis and some biochemical tests proved that 10 isolates belonged to G. vaginalis bacteria, while the results of genetic diagnosis of the 16S rRNA gene proved that 25 bacterial isolates belonged to G. vaginalis bacteria, which were found to be present in vaginal secretion samples. The presence of the SI gene, responsible for the production of the Sialidase enzyme, was investigated in all vaginal secretion samples of pregnant women suffering from bacterial vaginosis using the polymerase chain reaction (PCR) technique. The results showed the presence of the gene in 24 vaginal secretion samples, and the results also showed that 24 G. vaginalis isolates carried this gene.

The effect of the aqueous extract of horsetail (Equisetum arvense) on induced renal failure in rats was studied using a single low dose of mercury dichloride (1.5 mg/kg) injected intramuscularly, then the rats were treated with different doses of the aqueous extract of horsetail (5 mg, 7.5 mg, 10 mg) at a rate of one daily dose given orally. The changes in serum urea concentration, serum creatinine concentration, serum potassium ion concentration, serum sodium ion concentration were measured after different periods (3, 10, 30, 60) days. The results of the research showed that the injected dose of mercury dichloride (1.5 mg/kg) caused renal failure, the signs of which were a significant increase (p<0.01) in serum urea concentration (46.14 mg/100 ml), serum creatinine concentration (2.6 mg/100 ml), serum potassium ion concentration (5.46 mg/100 ml) and serum sodium ion concentration (152.83 mg/100 ml) compared to their normal rates (20.76 mg/100 ml, 0.61 mg/100 ml, 4.41 mg/100 ml). 100 ml, 151.33). This was accompanied by a decrease in the weights of the animals, which continued with the increase in time periods. The results indicated that the aqueous extract of horsetail plant after the occurrence of kidney failure caused a significant decrease in the concentrations of serum urea, serum creatinine, serum potassium ion, and serum sodium ion, and this significant decrease continued over time. The results of the research showed that the best concentration that caused a significant decrease (p<0.01) was the concentration of 7.5 mg, as the serum urea concentration decreased to 28.6 mg/100 ml, serum creatinine concentration 0.69 mg/100 ml, serum potassium ion concentration 5.12 mg/100 ml, and serum sodium ion concentration 151.7 mg/100 ml after 60 days of treatment compared to the concentration values ​​of the group treated with mercury dichloride only, and this was accompanied by a clear improvement in the weights of the rats. Therefore, these results are considered clear evidence of the possibility of treating kidney failure using this herbal plant.

A factorial experiment was conducted in one of the plastic houses affiliated to the College of Agriculture - University of Kufa in the summer season on 1\5\2014 to show the effect of the cutting location on the stem using three types (terminal, middle and basal) and the growth medium using two types of growth media (cultivating cuttings in the mixture alone and cultivating them in a medium consisting of (1 mixture: 2 peat moss) in rooting the cuttings and vegetative growth of the Conocarpus plant using the Randomized Complete Block Design (R.C.B.D) and with three replicates. The results showed the superiority of the terminal cuttings significantly by giving them the highest rooting percentage (93.39%) and the largest number of roots (32.53/cutting) and a significant increase in growth characteristics (length of growing roots (cm), average height of the seedling (cm), number of leaves/seedling, leaf area (cm2), dry weight of leaves (g), chlorophyll content of leaves) (mg.100 gm-1) (12.45 cm, 33.01 cm, 35.56 leaves/cutting, 19.56 cm2, 11.91 gm, 193.19 mg.100 gm-1) respectively. Cultivation in the medium (1 mixture: 2 peat moss) gave a significant increase in the studied traits.

In this study, E. coli (negative) bacteria were eliminated (killed) in wastewater by irradiating ultraviolet rays in the presence of MnO2 as a catalyst by using a bioreactor designed for this purpose, which is an effective method for killing bacteria in water contaminated with it.

Initial experiments were conducted to determine the optimum conditions that give high efficiency in purifying water from bacteria, such as the effect of the acid function and the amount of the catalyst.

The results indicate that the efficiency of photo-oxidation in the presence of the catalyst is higher than in its absence, as the efficiency of eliminating bacteria was 95% using a catalyst weight of 0.2) g and PH = 5.