Iraqi Journal of Laser

Breast mass is by far the most important clinical problem that concerns the breast today.This study was carried out to evaluate diode laser as a cutting tool in breast mass excision and as a hemostatic tool for coagulation during surgery. Using810nmdiode laser with optical fiber600μmin diameterof conicaltip,udder (cow's breast) tissue,andthreefemale patients(mean age of 35.5 y with clinically palpable breast mass)had been used in this study. The patients were followed up regularlypostoperatively.In preliminary work on udder tissue,the power needed for cutting and excision was 15W(powerdensity= 5.3 kW/cm2). The time consumed for excision of a piece of udder tissue,40×10×3 mm in dimensions was 5 min.Thedepthrange of cutting was 2-3 mm.The speedof cutting was 0.6 cm/s.In the clinical work,the powerneeded for excisionwas 15W(5.3 kW/cm2power density).Powerused for coagulation was ranging between 10-15Wdepending on diameter of blood vessel,the overall blood loss during surgery and postoperatively appeared to be less thanthat in conventional method. Thecontact laser scalpel produced clearappearance of surgical margin and saved dissection. Hemostaticcapability assured clear anatomical visualization and few ligatures. Noclinical evidence of infection at the wound site postoperatively. The results showed that thediode laser is effective in minimizing the intra-operative blood loss, postoperative blood loss, andeffective in reducing the postoperative pain, swelling and edema. On follow up the patients were presented with relatively better healing progress, no wound infections, and no need for strong pain killer postoperatively

In humans, Pseudomonas aeruginosa is the second most frequent gram negative nosocomial pathogen in hospitals and has the highest case-fatalityrate of all hospital-acquired bacteremia because of the hardy resistance of these bacteriato mechanical cleansing as well as to disinfectant, and many antibiotics. The susceptibility of bacteria against the antibiotics is modulated by several local factors such as temperature which modified drug efficacy, so thisstudy was carried out to evaluate the effect of different temperature (20,42,45)Ċon the susceptibility of Pseudomonas aeruginosa to the minimum inhibitory concentrations (MIC) of the antimicrobial agents before and after irradiation. The samples collected from 150 persons suffering from burns-wounds infections, thirty-five isolates of pseudomonas aeruginosa bacteria were obtained depending on morphological and biochemical tests. Following exposure of Pseudomonas aeruginosa isolates to the diode laser with 805nm wavelength,3W output power and (5,10,15) minutes exposure times in combination with different temperature and different concentrations of ( cefotaxim, amikacin, chloramphenicol) antibiotics, highly observable change in the MIC value was achieved , the bacterial isolates became sensitive to chloramphenicol at the threeexposure times and 100% killing of the cells was observed at 15 minutes exposure time at temperature 45Ċ in absence of the antibiotics. In conclusion, 3W diode laser in combination with temperature 45Ċ was the best condition that reduces the MIC value, and killing bacteria at 10 minutes exposure time.

An isolate of Leishmania majorwas grown on the semisolid medium and incubatedat 26ºC. The isolate was irradiated by He: Ne laser (632.8 nm, 10 mW) at exposure times (5, 10, 15, 20, 25, 30) minutes in their respective order. The unirradiated groups represent control group.Growth rate and percentage of viability were examined during six days after irradiation.The change in these two parameters reflects the effect of irradiation on the parasite.The results refers that the general growth effected by irradiation in comparison with un irradiation group, The growth rate of parasite decrease with increasing the exposure time in comparison with control group. Parasite viability decrease with irradiation and the percentage of living cell decrease with increasing of exposure time. High doses of laser at long exposure times increase the probability of damage this chromophore and leading to the negative effect on the parasite