Iraqi Journal of Bioscience and Biomedical

This study was designed to produce silver nanoparticles from the supernatant of Pseudomonas aeruginosa cultures propagated under aerobic and anaerobic conditions. Silver nitrate solution at concentration 100 mM was added to the supernatants, then thermal treatment at 80 ºC for 4 h. The heated supernatants were then burned at 600 ºC for 3h. Silver nanoparticles products were characterized using qualitative and quantitative tests. Morphological observation of the supernatants color change was made after adding silver nitrate and heating treatment.. The color of supernatant was changed into dark brown and light brown for each aerobic and anaerobic cultures supernatants, respectively. Quantitative tests included the analytical measurements for determination the ƛmax for aerobic and anaerobic supernatants after heating treatment in range of 300-500 nm to record the silver nanoparticles production and two peaks were obtained at 390 and 420nm, respectively. FTIR analysis schemes of the supernatants after thermal treatment at 80 ºC showed differences in composition of active compounds since more groups in the supernatant of aerobic supernatant were noted. No specific active groups were observed after burning the samples. Scanning electron microscopy (SEM) images of burned supernatants were conducted and revealed the presence of small nanoparticles for aerobic treatment in comparison with anaerobes. The results of atomic force microscopy(AFM) showed that aerobic the treatment was produced a silver nanoparticles of approximate diameters of 20-50nm compared with anaerobic treatment (50-100nm).

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Cancer is a major cause of mortality and morbidity globally and overall survival rate is still low despite advances in surgery, radiotherapy, and chemotherapy. Plants have played a significant role in the treatment of cancer and infectious diseases for a very long time. Annona muricata has been widely used in the treatment of cancer and diseases in many countries. This study was designed to evaluate the cytotoxic effects of Annona muricata ethanolic extracts on (MAD_MB_231) human breast cancer cell lines and lung cancer cell line (A549). Various concentrations of ethanolic extracts (50, 100,150 and 200 µg/mL) were prepared with ethanolic solvents and used to treat cell lines after 24 h. exposure by MTT assay. The results showed the effect of extract after 24- hour treatment is a dose dependent and has the most effective anticancer activity on the lung cells line with IC50 Values of The IC50 was 134.6 mg/ml for breast cancer cell lines and The IC50 was (124.6) mg/ml for lung cancer cell line. the current study showed the highly effective action of the ethanolic crude leaves extract of Annona muricata and can be used in the management and treatment of cancer.

In this study, hundred (100) specimens were collected from patients of both gender and different age groups in three government hospitals in Baghdad/Iraq from January 2022 to May 2022. Samples were diagnosed by routine, biochemical tests, and Vitek- 2 system device to diagnose the Pseudomonas aeruginosa, the results showed that 32 (32%) were P. aeruginosa bacteria. Antibiotic susceptibility testing was performed for all 32 isolated P. aeruginosa pathogenic bacteria and sensitivity was performed for the aminoglycoside group (Gentamycin, Amikacin, streptomycin) of antibiotics to detect isolates sensitivity or resistance to antibiotics circulating in health institutions, P. aeruginosa showed the highest antibiotic resistance to Gentamicin: 40.6%, streptomycin: 37.5%, Amikacin: 25%. In the present study our target isolates of P. aeruginosa which is resistant to Gentamicin, Amikacin and streptomycin according to our result we found the most isolates which resistant to Gentamicin, Amikacin and streptomycin are P1, P5, P8, P9, P14, P17, P18, P19, P22, P29.

The aim of study was to determination the kinetics adsorption of heavy metals from water samples obtained after sand filtration stage in AL-Wihda station of drinking water production in Baghdad by using three types of synthesized nanocomposite resins of tannin loaded with TiO2NPs in order to select the best formula. The photocatalytic degradation and photochemical characteristics of TiO2NPs on different organic and inorganic pollutants in water types were exploited. form Acacia nilotica leaves powder and synthesized the tannin resin (TR). Three types of nancomposites of tannin resin (TR) loaded with TiO2NPs (TR-TiO2-1, TR-TiO2-2, and TR-TiO2-3) synthesized by adding the TiO2NPs before the formalin (TR-TiO2-1) and after formalin addition (TR-TiO2-2) and chemical green synthesis of TiO2NPs from TiCl4 by TR solution (TR-TiO2-3). The results showed that the nanocomposite TR-TiO2 -2 was the most efficient in removing the heavy metals pollutants from water samples compared to the Iraqi and Word Health Organization (WHO) standareds. Four metals ( Mn, Cr, Cd and Hg) were selected for monitoring their removal from water samples after treating with TR-TiO2 -2 composite. The remaining concemtration of Mn was 0.03 mg/L after 10 min of treatment; Cr, 0.006 mg/L after 50 min; Cd and Hg were not detected in water samples before and after treatments. Study of the adsorption kinetics of heavy metals for both equilibrium and the relationship of mass adsorption with time was showed that the maximum adsorption at the fixed times for each mineral especially the Mn and Cr, where the equilibrium state of the maximum concentrations of the adsorbed material was obtained on the nanocomposite

In a group of women with early-stage breast cancer in Baghdad/Iraq, this study has been done to assess the impact of certain SNPs situated on RAD51 genes on its repair effects on DNA damage. Between November 2020 and March 2021, (100) FFPE samples from breast cancer patients who were diagnosed in the early stages of the disease were obtained at the Tumor Teaching Hospital in the Medical City and the Al-Amal National Hospital in Baghdad. As a control group, (50) samples from healthy women were also obtained. All of the study samples' DNA was taken in order to find SNPs, and their ages varied from (40 to) 60 years. Additionally, DNA sequence analysis was used to identify single nucleotide polymorphisms (SNPs) in exon 6 of the RAD51 gene. Then, these exons' nucleotide sequences were matched with NCBI and the control group (healthy women). In exon 6 of the RAD51 gene, one polymorphism, rs121917739 GA, was discovered