Al-Mustansiriyah Journal of Science

In this study we tried to convert the chemical energy to electrical energy by using microbial fuel cell (MFC) consist of two chambers (anode and cathode) in presence of bacteria (Escherichia coli, pseudomonas aeroginosa) and yeast (Saccharomyces cervesia)in the anode chamber to generate electrons. The system was started with glucose concentration 5gm/l in different pH value from (5-8). From the results we get the great generation of electricity with S. cervesiaat pH 5 and the maximum voltage was 833mv. In case of bacteria that used in our experiment, the suitable pH for generation the electricity was.(7)

This study was compared the antimicrobial effectiveness of Lawsonia inermis plant extract (alcoholic, aqueous and silver nanoparticles) on the growth of different types of Gve+ bacteria and Gve- bacteria at concentration 80 mg /L. It was noted that the ( alcoholic, hot and cold water) extract have highest impact recorded in the growth of bacteria S.aurous with inhibition zone (26mm) followed by the bacteria P.aueroginosa at inhibition zone reached (22mm) and finally the yeast C. albicans at inhibition zone reached (17mm), while silver nano-particles of the plant extract the most influence on the bacteria, it gave the highest Inhibition area reached (30mm) on the growth of the S. aurous, followed by the inhibition area of P.aueroginosa reached (26mm) then Strep. pyogein (25mm), and finally the yeast C. albicans reached (18 mm). With regard to the complex (alcoholic extract and antibiotic Amoxylein) its effects has varied upon microorganisms, there is a synergistic effect on the growth of the yeast C. albicans at inhibition zone (30mm) while it has been observed inhibitory effect of this complex on the growth of p. aeroginosa with inhibition zone amounted to (15mm), but did not show any significant impact on the growth of both bacteria (K. pneumonia, E.coli Strep. Pyogein& P. aeroginosa,), while the (alcoholic extract and antibiotic Erythromycin) showed inhibitory and synergistically effect on each of the bacteria (E.coli, S. aurous, yeast C. albicans and P. aeroginosa) respectively. According to obtained results the antibiotic Erythromycin shows higher inhibitory effect than antibiotic Amoxylein. It has also been investigated the lower values of absorbance at the wavelength of 260 nm with increasing the concentration of crude alcoholic extract, this reveled the ability of henna extract in the removal of free radicals liberated from photolysis of hydrogen peroxide compound .

Locally produced cheese which called (Gibin Al arab) is one of the most common dairy products in Iraq, it has an economic importance and great social value. This research aimed to identify yeast species from locally produced cheese (Gibin Al Arab) in Diyala city which traditionally made and sold in markets of old town in Baquba, and study some of virulence factors (Esterase production, Phospholipase and Hemolytic production) of yeasts belong to genus of Candida . All cheese samples showed contamination with varying number of yeast, total 88 yeast isolates obtained from 70 cheese samples, they were Geotrichum candidum(20.5%), Rhodotorela species(19.4%), Candida parapsilosis (18%), Candida albicans (13.6%), Candida tropicalis (10.5%), Candida krusei (8%), Saccharomyces cerevisice (3.3%) and mixed yeast (un identified) at rate of (6.7%). Species of Candida formed half of the total isolates and the most prevalent isolate of Candida spp. was Candida parapsilosis .According to the results determining of (Esterase production, Phospholipase and Hemolytic production) as a virulence factors identifying Candida spp. these activities referred that all isolates of Candida spp. show one or more of these activities and that isolates of medically important species Candida albicans were the most virulent isolates. this referred to the importance of take attention about consuming of such types of dairy products and need for applying more hygienic measures during handling, processing of milk and form of storage and/or selling of cheese.

Pesticide residues have been found in various fruits and vegetables. This studycollected 24 samples and reported a method based on High Performance Liquid Chromatography (HPLC). For determination of pesticide residues used in Some fruits which were collected from different markets of Baghdad city to make) 24) samples from (peel , core, mixture) for each type of fruits markets as: Oranges( Egypt , Africa), Pomegranate ( Egypt), Mango ( Kenya ), Pears (China), Plum fruits ( Africa ) , Kiwi (Turkey ) .That detective of ( 5 ) different pesticides (diazinon, malathion, chlorpyrifos ,parathion and cypermethrin). The results were detected of multi-residues of pesticides on the fruit in ( peel , core , mixture ) may be in the limit of Maximum residue limits(MRL) or higher of it . The pesticides detected that exceeding the limits are: cypermethrin in Kiwi (peel, core and mixture)at(0.204,0.038, 0.537), in pomegranate detected in (peel and mixture) at (0.509,0.189) mg/kg , Diazinondetccted in Egyptian orange in (peel ,core and mixture) at (0.031,0.207, 0.099) mg/kg . Malathion and Parathion was not detected at any type of fruits and that results was compared with codex of FAO/WHO(2013). That data is important to monitor residues in food and to fill gaps in current knowledge would be helpful in assessing human exposure risks from ingestion of contaminated Fruits Imported to our country.

An attempt has been made to determine the antimicrobial activity of vitamin D; D3 & D2 against clinical bacterial isolates as well as perform a comparative analytical study between the effects of both forms of vitamin. The ability of vitamin D (both D2 ergocalciferol& D3 cholecalciferol ) to inhibit bacterial growth of some clinical isolates have been tested . Forty - three pathogenic bacterial isolates (Gr+ ,Gr- ) have been identified from fifty - five specimen was collected from different sources ; 24 urine, 17 sputum , 9 blood , 5 skin at Al-Kindey hospital for a period of two months . Antibiotic sensitivity was carried out towards 12 different antibiotic discs. The most resistant isolates have been chosen to be tested in the study. Two bacterial suspensions of the selected isolates have been prepared; the first was adjusted to McFarland standard No. 0.5 (1 ×10 8 CFU /mL); the second = 1×1010 CFU /mL. Three concentrations of both vitamins have been prepared; 50,000, 70,000 and 90,000 IU/mL as well as the control ( solvent only ). Antibacterial activity has been examined by using agar diffusion (pore plating method) to determine the most effective concentration among the three concentrations of the two forms of vitamin D. Results were suggesting the important role of vitamin D specially D3 as antibacterial agent .The third concentration (90,000) IU/mL was causing the largest inhibition zone with all tested isolates even with the high turbidity culture (1010CFU/mL) ,followed by the second one (70,000) IU/mL, the lower inhibitor concentration was (50,000) IU/mL. Significant differences have been appeared among the measurements of the diameters of inhibition zones towards three vitamin concentrations when compared one to another and to control.

Klebsiella pneumoniae is dangerous pathogens that can cause severe diseases. This study included isolation of 50 isolates of K.pneumoniae from different clinical sources from different hospitals in Baghdad city, the number and percentage of isolates according to the sources (urine, blood, sputum, burns, ear swabs, pus, wounds and stool ) were 22(44%), 11(22%), 4(8%), 4(8%), 3(6%), 3(6%), 2(4%) and 1(2%) respectively. The ability to form biofilm was carried out using Tissue culture plate methods (-TCP-). The results showed that 80% of the isolates were producer biofilm; the genetic study was used to detect the presence of mrkA, mrkD genes that are believed to be responsible of biofilm production. The ratio was mrkA 87.5% and mrkD 50% before exposure to U.V.light to reduce to 43.7% mrkA and 18.7% mrkD in isolates after exposure to U.V.light source.

Biochemical changes occurring in two types of diabetic patients have been investigated to identify the correlations between these changes and type 1 and type 11 of diabetic patients, in comparison with that of normal healthy, sera of 68 diabetic patient with type 1 and type 11were used to estimate the L-fucose level and other related parameters. These parameters measured throughout this project are: TF, PBF, TP, TFYTP ratio and PBF\TP. As final approach, the author concluded that fucose level together with its related parameters could be a biochemical markers for early assessment of re­sponse to the therapy served.

Patients with β-thalassemia may have an increased risk for diabetes mellitus and cardiovascular diseases due to high level of iron which may lead to insulin resistanceand metabolic syndrome. So this study aimed to evaluate the levels of lipids profile in Iraqi female patients with β-thalassemia. Forty twofemale (age 15-30) years were enrolled in this study. Blood was collected and the sera were separated from (22) female patients with β-thalassemia who were attended the Ibn-Al-Baladi hospital from September 2012 to January 2013 and (20) healthy subject as a control group. Body mass index (BMI), lipid profile, FSG, insulin, insulin resistance, insulin sensitivity, B-cell function, iron, atherogenic index of serum were estimated.

The results showed the presence of a significant increase in serum iron and significant decrease in insulin, B-cell function, LDL, VLDL, and TC in serum of patients with β-thalassemia when compared with control group. BMI also showed a significant decrease in patients when compared with the controls. Serum Insulin resistance, insulin sensitivity, HDL, TG, AIS, and FSG showed no-significant differences in patients with β-thalassemia when compared with control group. We concluded there was no metabolic syndrome in female patients with β-thalassemia.

In this research new tridentate Schiff base ligand (S)-4-(methylthio)-2-((1-(pyridin-2-yl)ethylidene)amino) butanoic acid (HL), have been prepared from condensation of (2-acetylpyridine) with methionine amino acid . A coordinated complexes of manganese (II), cobalt(II), nickel(II), copper(II) and zinc(II) have also been prepared. The prepared ligand and its complexes characterized depending upon (FTIR &Uv-Vis) spectroscopies, elemental microanalysis (C.H.N.S and M) ,(1H and13C) N.M.R spectroscopy, mass spectrum, flame atomic absorption, magnetic measurements and molar conductance. The results of different studies proved that new Schiff base ligand acts as a tridentate ONN donor in all the prepared complexes, as well as the results provided that the geometry of all prepared complexes was octahedral with (1:2) (M:L) ration , except Zn(II) complex which obtained tetrahedral geometry with (1:1) (M:L) ratio.

The binding modes of a new zinc complex derived from 3-(5-bromo-2-hydroxybenzylideneamino)-2-(5-bromo-2-hydroxyphenyl)-2,3-dihydroquinazoline-4(1H)-one withdivalent zinc was studied using both experimental and computational chemistry. 1H NMR, IR, molar ratio and atomic absorption were used to predict the characterization of zinc complex. Hartree–Fock (HF) method was selected to determine the HOMO-LUMO gap of several possibilities of binding sites to describe the more stable structure which reflects the more possible coordination sites of ligand to Zn+2 ion. Good matching between theoretical and experimental results was found which concluded the tetra dentate behavior of the ligand.

This research includes preparation of (2-chloro-N-p-tolylacetamide) (1) from the reaction of (p-aminotoluene) with chloro acetyl chloride. Compound (1) reacted with thiosemi carbazide and gave compound (2), and when compound (1) reacted with semicarbazide gave compound (3). While when compound (1) reacted with thiourea it produced compound (4).

Compounds (2-4) when reacted with appropriate aromatic aldehydes or ketones produced Shiff bass (5-16), which in turn reacted with chloro acetyl chloride in the present of tri ethyl amine and dioxin gave β-lactam derivatives (14-22). The structures of these compounds were characterized from their melting points, FT-IR, and NMR.

This research includes preparation of (2-chloro-N-p-tolylacetamide) (1) from the reaction of (p-aminotoluene) with chloro acetyl chloride. Compound (1) reacted with thiosemi carbazide and gave compound (2), and when compound (1) reacted with semicarbazide gave compound (3). While when compound (1) reacted with thiourea it produced compound (4).

Compounds (2-4) when reacted with appropriate aromatic aldehydes or ketones produced Shiff bass (5-16), which in turn reacted with chloro acetyl chloride in the present of tri ethyl amine and dioxin gave β-lactam derivatives (14-22). The structures of these compounds were characterized from their melting points, FT-IR, and NMR.

In this study binary and ternary solutions are prepared by using the sodium acetate concentrations (0.1, 0.125, 0.2, 0.25, 0.4, 0.5, 0.8, 1 M) in water and acetone –water mixtures .The important parameters such as apparent molal volume, the partial molal volume transfer, apparent molal compressibility, free energy of activation of viscous flow and thermodynamic activation parameter (enthalpy and entropy) determined of sodium acetate in water , 20%, 40% ,60% and 80% V/V acetone –water mixtures at 298.15K, 303.15K, and 308.15K from density and viscosity measurements espectively. The limiting apparent molal volumes and experimental slopes were derived from the Masson equation, have been interpreted in terms of solute–solvent and solute–solute interactions respectively. The viscosity data were analyzed using theJones–Dole equation and the derived parameter B - coefficient has also been interpreted in terms of solute–solvent interactions in the solutions.