University of Thi-Qar Journal of Science

power plant emissions upon two species of plant Alhagi mourorum and Suaeda vermiculata which growth in the region close to Thermal power plant. triplicater samples were collected seasonllys from autumn 2016 up to summer 2017 one time from each season. three station were selected in the study area to execute this study meters. The first station distances 400 meters from the thermal power station, The second station is located 800 meters from the thermal power plant . While, the third station distances 1200 meters from the thermal power plant . Three stations were with prevailing wind direction (north wind) , while control station situated far from the thermal power plant about 12 km. the concentratios of heavy metals Cd, Cu, Pb and Zn were estimate in root and shoot systems for both plants, their concentration in root system more higher than their concentrations in shoot system. its concluded from the present study that thermal electric power station emissions affected upon the plants which growth in the surrounding region with thermal power plant.

The current study was conducted in Dhi Qar governorate, Iraq to determine the main types of microorganisms that cause female genital tract infection and the extent of the effect of the level of sex hormones (prolactin, progesterone, estrogen) on the presence of infection , 245 samples were collected for both vaginal swabs and blood (where take a vaginal swab and blood from every woman) , where 150 women were pregnant and 95 women non-pregnant women , aged 16-45 years .

Among non-pregnant women, most were women who had a reproductive tract infection within the leuteal phases , where our study included 58 women at leuteal phase , 20 women were in follicular phase , 17 women within ovulation phase of the menstrual cycle .

The study showed that Candida albicans , Staphylococcus aureus , Escherichia coli , Gardnerella vaginalis , Klebsiella pneumonia , Staphylococcus epidermids , Enterobacter cloaceae were isolated from women in follicular phase , the diversity of infections was lower in women in the ovulation stage, where they were isolated only Candida albicans , Staphylococcus aureus , Escherichia coli , Gardnerella vaginalis ,and from women with leuteal phase was obtained Candida albicans , Staphylococcus aureus , Escherichia col ,Gardnerella vaginalis , Klebsiella pneumonia , Staphylococcus epidermids , Proteus mirabilis, Pseudomonas aeruginosa , the precence of all type of pathogens in our study was associated with elevated estrogen and progesterone levels except the growth of Staphylococcus epidermids , Proteus mirabilis, Pseudomonas aeruginosa.

worldwide and is associated with gastric disorders. H. pylori is genetically unstable and this reflected on its virulence factors and type of diseases. Cytotoxin associated gene A (CagA) product is a major virulence factor is thought to be associated with gastric diseases. In the present study, we used RT-PCR for rapid detection of H. pylori infection in peptic ulcer patient, the determination of CagA gene directly from gastric biopsy specimens and determining relationship between gastric cancer and H. pylori. Gastric biopsy were collected from 54 patients with disorders in digestive system from AL-Hussein teaching hospital. The results of current study showed that the presence of 16SrRNA for H. pylori was 21(38.9%), 15 of 24(62.5%) were males and 6 of 30(20.0%) were females, whereas the percentage of CagA gene was 18(33.3%), 12 of 24 (50%) were males and 6 of 30 (20%) were females by using Real time quantitative PCR in 54 biopsy specimens. These result revealed a significant difference between males and females. Our study indicated that CagA gene RT-PCR is the most specific for the detection of H. pylori in gastric biopsy specimens in patients with gastric cancer compared with 16SrRNA gene . There were significant differences (p≤0.05) in CagA positive rate, among different diseases. In this study the presence of H. pylori in patients with Gastric cancer and peptic ulcer has been demonstrated by using RT-PCR .

Dental caries is one of an important common human infectious disease that can lead to loss of tooth structure, it is occur due to the metabolic activation of the plague microorganisms especially Streptococcus mutans bacterium. Eighteen samples from saliva and dental plaque were collected from patients with dental caries active at ages from (7-25) years from Specialized dental hospital and dental clinics at Thi-Qar province during the period from November to December 2016.Thirty-three isolates belong Streptococcus mutans were identified by cultural methods, biochemical tests and Api 20 Strep. system, while twenty-eight isolates identified by using Polymerase Chain Reaction (PCR) technique and sequencing of 16S rRNA gene with 507 bp, whereas by using 16S rRNA gene the result confirmed that these isolates were belong to Streptococcus mutans. The aim of study is Isolation and identification of Streptococcus mutans bacterium from the dental caries and dental biofilm patients at Thi-Qar province by of 16S rRNA gene.

molecular features, as well as the antimicrobial susceptibilities of Vibrio.fluvialis isolated from diarrheal (children) in Nasiriya city from (July 2016 to February2017) . A total of (20) samples of Vibrio. fluvialis were collected and analyzed by bacteriological, molecular and antimicrobial methods. Green and yellow bacterial colonies were recovered on thiosulfate citrate bile salt sucrose agar plates. The phenotypic characteristics of the isolates, including morphological, physiological and biochemical traits were determined and confirmed using API 20E system. Following isolation, the 16S rRNA gene specific for the genus Vibrio.fluvailis was investigated by using PCR. Antimicrobial susceptibility to 10 antimicrobial agents was determined by the disc agar-diffusion method .In this study the isolates were more resistance to polymaxin B (70%) & streptomycin (65%) and tetracycline (60%),while doxycycline was (60%) ,ampicillin and chloramphenicol (55%) ,while trimethoprim was resistance (50%).while was more sensitative to Nalidixic acid &Azithromycin (35%)and ciprofloxacin (45%). The study showed an increase presences of Vibrio.fluvialis in diarrheal children &ahight resistance of many antimicrobial agent that importance to find suitable methods to control the infection transmission.

Norovirus consided as the second cause of viral diarrhea in children.The current study included examination of 200 (115 females and 85 males)stool sample of diarrhea and abdominal pain suffering children, less than 5 years of age.The samples were divided into nine groups according to the age of the children: 1-2, 3-5, 6-8, 9-11, 12-17, 18-23, 24-35, 36-48, 50-55 month. Stool samples were divided for two part, the first part for the molecular diagnosis of virus by real time PCR and then gene sequences of virus and the second part used for ELISA test. Results of real time PCR test revealed that 35 positive sample out of 200 with a percentage of (17.5%).( AccuZo1TM total RNA extraction kit(Bioneer, Korea was used in this test). Real time PCR results showed that Depend on age groups the highly morbidity between 18-23 months (25.8 %) and there was significant differences between age groups on probability ≤0.05. Results showed that high percentage of infection between children depend on artificial nutrition Top valuewas (21.7%) and the low percentage of infection in children depend on mixed nutrition (breast milk and cereal meals) was (12.6%). ELISA test results included 25 positive samples from total of 200 samples percentage was (12.5%). The sensitivity of this test was 71.4% while specifity was 94% compare to Real time PCR. ELISA test showed that the age group (18-23 months) was (25.8%). with a significant difference at (P>0.05). Artificial feeding is higher than (15.2%) and normal feeding is lower (9.7%). From the positive samples 8 samples sent for gene sequences, four samples were revealed compatibility to GII.2, three samples to GII.4 and one sample compatible with GΙI.3. depend on base data of NCBI.Key words: Norovirus, children, Thi-Qar, real time PCR, Phylogenic.

The present study was carried out in the Labs of collage of education for pure science and Center for Diabetes and Endocrinology of the Health Directorate in Nassiriya city, during period from January 2017 to end July of the same year. The immune status investigates for patients with diabetes type I and type II by measuring the levels of cytokines (TGFβ1, IL4, IL6,IL17 ) in sera using a technique enzyme-linked immune Sorbent adsorptive (ELISA). The study included 88 subjects with (34) type I, (34) II diabetes and (20) were healthy control. The statistical analysis showed that a high significant increase (P ≤ 0.05) in serum IL6 and IL17 as the rate of concentration of IL-6 in patients (33.07 pg / ml) compared to the control group (4.68 pg/ml) with significant difference (0.00), while IL-17 concentration (113.66 pg / ml) for patients compared with the healthy control (43.67 pg / ml ) with a significant difference (0.01) in type I patients. While a highly significant (p<0.05) of (TGFβ1 , IL-6, and IL-17 ) in patients with type 2 diabetes compared with the concentration in IL – 4 and a healthy control group. The highest concentration recorded was (132.26 , 24.69 and102.02 pg/ml) respectively, compared to the other groups . These results revealed that the excessive presence of cytokines might play a role in diabetic in Nassryain population.

This study was aimed to determine the relationship between ABO blood group with hemoglobin level ,glucose level and urinary track infection in pregnant women inAL- Naissary city Thi-Qar province ,Iraq .Atotal of 100 pregnant women have beenfound to be screened in this study .Blood samples were collected to examine blood group Hb,and glucose level Also,urine samples were collected to examine urinary track infection .The results showed a significant decrease in hemoglobin level in pregnant women which carried the blood group O when compared with the rest groups(P<0.029) .But there was a significant increase in urinary track infection in the same blood group(P<0.012) . From the another side the blood group type B have significant increase in glucose level(P<0.027) .The finding of the present study was that we the blood group type O was more susceptible to urinary track infection and low level Hb concentration .but the blood group type B was more susceptible to infected with Diabetes.

Atrazine (ATZ)has been associated with its capability to interact and damage DNA in neuronal cells. However, the molecular mechanisms of ATZ are not fully understood. In vitro and In vivo studies indicated that atrazine induces cytotoxicity and oxidative stress in human and animals. This study sought to determine the role of two antioxidants against the toxic impact of atrazine. Main Methods: N27 cell line pretreated to N-acetyl Cysteine NAC or Ascorbic acid AA alone or in combination before 24h atrazine exposure. Multiple intercellular antioxidant parameters measured such as (GSH, GSSG, MDA). Genotoxicity represented by single gel electrophoreses assay (comet assay). Results: the outcomes of this work demonstrated that higher protection against atrazine exposure at combination of NAC and AA comparing to the single exposure to each. The antioxidant acted in synergistic way to rescue cells from reactive oxygen species generated from atrazine exposure. DNA breaking or damage also prepared in better way in combination exposure comparing to the single exposure. Conclusion: the evidence of atrazine increase the oxidative damage have been approved by enhancing defense system to increase cell availability and DNA repair. While antioxidant (NAC&AA) prevent oxidative damage that causes by atrazine via increase cell viabilities and DNA fix.

The present study was conducted for the period from November 2011 to October 2012. Three sites were identified in this study to measure some of the physical and chemical properties of the main outfall drain water, the passer Al-Qadisiyah Governorate to determine the quality of the public water, physical and chemical, and its suitability for irrigation. The results of the current study showed that the values of the air temperature ranged between (4 - 45 C°) and water (9.5-30C°). The pH values were bent to the basic side and ranged from (7.7-8.4), dissolved oxygen values (5.1-10.8) mg / l and electrical conductivity was ranged from(3380-11520) μs / cm, Salinity ranged between(2.1 -7.3g / L), where water is classified as oligohalic, The total dissolved solids values ranged between (3310 - 7650) mg / L. The high alkalinity in the study , total alkalinity ranged from (1140 -2280mg CaCo3/ L), Calcium ions were recorded (131.4 -320-mg CaCo3 / L), and Magnesium (135.9-401.2 mg CaCo3/ L). The values of sodium exceeded the Iraqi and international standard limits and ranged between (708.7-1256mg/l), Potassium values ranged between (13.3 -28.2mg / L), The values of chloride were high, ranging between (849.7 -1939.3mg / L) and sulphate (734.8-1074.2) mg / l), The ratio of sodium adsorption exceeded the permissible limits for irrigation of agricultural crops according to the classification of the American salinity laboratory (1954) ranging from (35.973.5 %). The results of the study showed that the MOD water is physically and chemically unfit for the determinants.

In this study isolate 134 organism from tap water at the time reaserach through the seasens winter and Sumer , to determinate pathogenic bacteria of drinking water from different regions in Nasseriya city from October 2014 to September 2015 , many people do not have access to clean and safe drinking water and many die of waterborne bacterial infections, Salmonella typhi ,Salmonella typhimurium Streptococcus faecalis ,Esherichia coli ,Aeromonas hydrophilli , Campylobacter jejuni ,Enterococcus faecalis ,Staphylococcus aureus ,Citrobacter freundii,Citrobacter diversus , Vibrio cholerae Pseudomonas aeruginosa , Helicobacter pylori , Mycobacterium avium complex, (MAC is presented, focusing on the biology and ecology of the causal agents and on the diseases‘ characteristics and their life cycles in the environment, the different city ,that contain many microbial pathogenic in tap water,the main bacteria present in human feces for example Enterobacter cloacae ,Pseudomonas aerogenosa , important sources of bacterial fecal pollution of waters are also briefly indicatedin pollution and break point in pipe line ,the aim of this study was to isolate and identify bacteria from drinking water distributions system in Nasseriya city.

This paper elaborated transverse sections of leaves and stems of nineteen species belongs to 18 genera of Brassicaceae family. Anatomical characteristics were important in separation of taxa. The presence or absence of Collenchyma, Chlorenchyma and Pith, Mesophyll structure and epidermal surface were found to be important characters for the identification of Brassicaceae species. Two types of mesophyll have been recognized, dorsiventral (Bifacial) found in Sinaps alba L., Biscutella didyma L., Sameraria stylophora(Jaub. et Sp.) Boiss., Drabopsis nuda (Bel.) Stapf., Eruca sativa Mill., Capsella bursa-pastoris (L.) Medic., Calepina irregularis(Asso)Thell., Diplotaxis harra (Forssk.) Boiss., Sinapis arvensis L. and Nasturtium officinale R.Br. and isobilateral in the remaining species. Transverse sections of the midrib showed that most of species contains one vascular plants in all the species except Brassica oleracea L., Cheiranthus cheiri L., Crambe orientalis L., Draba bruniifolia Stev. and Sinaps alba L. which contains (1-3) vascular bundles. Collenchyma tissue present only in Diplotaxis harra, sinaps alba, Drabopsis nuda (Bel.) Stapf. Camelina rumelica, Cramba orientalis L., Cheiranthus cheiri L. and Cardaria drab Thell, it was higher in Cardaria drab was (150.50) um and lower in Crambe orientalis was (18.75) um. A diagnostic key based on combined stem and leaf were presented.

The study included isolating and diagnosing of Bacillus subtilis different soils at Thi Qar province. The ability of bacterial isolates to produce Endo-β-1,4-glucanase was determined after growing on carboxymethyl cellulose CMC medium, Using Congo red and NaCl. Enzyme Endo-β-1,4-glucanase isolates were identified using biochemical tests and VITEK2 as B. subtilis. Isolates were identified using 16S rRNA tests after extraction of DNA from isolates and amplified by PCR using 27F primers (Forward) and 1492R (Reverse) . All isolates in the current study were positive for this gene and the size of the gene for all isolates was at 1500 pb. After identifying the gene sequences and comparing them with the data available in the Gen Bank, NCBI data showed that they were new strains of B. subtilis strain m1 (MF449304) and B. subtilis strain M2 (MF449461) bacteria. Isolates were recorded in NCBI GenBank and were design for each evolutionary tree isolation by Software MEGA6. After the diagnosis of bacterial isolates, the ideal conditions for the production of Endo-β-1,4-glucanase were changed for incubation period, temperature, pH , Incubator Shake, carbon and nitrogen sources. Enzymatic efficacy was determined using of dinitro salicylic acid DNS detector to detect the glucose releasing glucose molecules Endo-β-1,4-glucanase. The enzyme was produced after growing of bacterial isolates of plant culture containing plant and cardboard residues as natural sources of carbon and alternative to costly industrial sources as a source of carbon at a concentration of 1% at 45 ° C for 48 hours .

This study was performed to detect the presence of CTX-M-1 gene in Klebseilla pneumonia and Escherchia coli are that causing urinary tract infections in pregnant women in Al- Nassyriah city , during the period from september (2016) to february (2017). Three hundred and thirty (n= 330) urine samples were collected and cultured on appropriate media for initial isolation. Morphological, conventional biochemical tests and API 20 system were performed to identify the pathological bacteria agents.Ninety samples showed positive culture, the most common isolates were E. coli 57 (63.3%) followed by K. pneumonia 21(23.3%) and 12(13.3%) from other Gram negative bacteria. ESBLs gene CTX-M-1(688 bp) was found in 22 isolates 17/57 (29.8%) in E.coli and 5/21 (23.8 %) in K. pneumonia.

Diabetes mellitus keeps on developing in worldwide pervasiveness and consume amount of health care resources. The management of diabetes incorporates capacity to change lifestyle, and physical activity.Objectives:Evaluate of management role (counting adherence) in control status and appraisal of adherence in control status of diabetic. Method: A cross-sectional design was used a convenience sample of 139 subjects with type 1, type 2 diabetes, ≥25 years old, included residents of the community in the city of Nasiriyah only registered in the Diabetes Center for both gender (62male and77female), between March and September 2016.Questionnaire sheet was used designed for collect data. Results: The study results indicated that the persons with a mean age of 50.01 ±12.4years, the cause of disease for participants' more than half of them are hereditary (n=88;63.3%), Considering the uncontrolled more than half of them (n = 102 ; 73.4% ), less than half of them is within 10-14.9years duration of disease (n=46; 33.1%), more than half of them are used double hypoglycemic drug and poorly controlled in HbA1c (n= 111 ; 79.9%), (n =83 ;59.6%); respectively; there is a significant association of HbA1c with type of drug, significant association between determinants and duration of disease and type of drug with level of p-value ≤ 0.05. Conclusions. The majority of subjects haven’t their diabetes controlled; their low self-efficacy, and decline self-management. Therefore, strategies to promote self-management for patients are essential components of diabetes education programs, skill-building interventions are critical to be able to manage their diabetes.

To assess the characteristics of 195 cases with nasal foreign bodies regarding the sex , age , types , site and complain at presentation. Materials and Methods : One hundred ninety five patients with nasal foreign bodies , their age range of 1-12 years old visiting the private clinic from March 2010 – June 2015 retrospectively analyzed. Results : The nasal foreign bodies mostly presented with males , the most common age group from 1-6 years and the most common foreign bodies were beads and plastic toys. Conclusions : Nasal foreign bodies one of the most common otolaryngological emergencies in pediatric practice , although it is not life threatening , it still need special care to prevent complications.

The ear infections are highly popular disease in the world. Chronic suppurative otitis media (CSOM) is one of the prevalent hearing problems, this infection of the middle ear may extend to cranium that can cause many serious complications if not treated duly. It is famed for its return enduring infection. The causative agents of bacteria or fungi may be cause the CSOM infection. Therefore, our study is mainly aimed to identify the bacterial isolates which causing of CSOM and detect some isolates of fungi which implicated in this ear infection with performed of the antimicrobial susceptibility test. The study was executed on people whose attending the outpatients clinic of ENT in AL-Mwanee General Hospital during the period from November, 2016 to May, 2017 in Basrah governorate. Ear swab samples were collected and processed from eighty five (85) patients of CSOM by the following of standard bacteriological procedures mainly, for isolation and identification the bacterial pathogens, in addition, common diagnosis methods was used to detect of some fungal isolates. The positive microbial growth cultures were seen in 59 cases with frequent of (69.41 %) and 26 cases were negative in frequent of (30.58%). Their ages ranged from 1-60 years with high incidence of CSOM in age groups ≤ 10 and 31-50 years old in percent's of (38.82 % and 24.70 %) respectively. Polymicrobial samples from the total isolates (89) which identified in this study were (82) aerobic bacterial isolates in frequent (92.13 %) and 7 fungal isolates in frequent.

Diabetes mellitus is a common disease in most parts of the world, the metabolic abnormalities of diabetes potentially affect bone metabolism, structure, mineral density, and bone resorption, as part of physiological bone turnover. Aim: This study aimed to estimate urinary total deoxypyridinoline as a biomarker of mature bone collagen degradation and bone resorption in type I diabetes mellitus patients and evaluate its relationship with fasting blood sugar, serum bone minerals profile and the duration of disease and also to investigate the degree of severity of bone resorption and the probability of fracture risk. Patients and methods:165patients with type I diabetes mellitus from Diabetes mellitus and Endocrine Disease Centre at AL -Nasiriyah city in Thi-Qar province, Iraq were studied.100 (60.6%) were female and 65(39.4%) were male, age was between(25-65) year, mean44.27±11.1,their duration with type 1 DM disease ranged from 5 months to 10 years, they were classified into three groups: the first group included 45 (27.3 %) patient their duration with type 1 DM were less than one year, the second group included 65 (39.4%) their duration with type I DM were 1 to 5 year and the third group included 55 (33.3%) patient their duration with disease were from 6 to 10 year. Fasting blood venous and first-morning urine samples were collected for biochemical investigation according to standard methods and Urine DPD was measured by the quantitative competitive enzyme immunoassay. Also, this study included 165 normal people selected as a control group.

Avian influenza is one of the important viral diseases in poultry that can cause serious economic losses in many countries around the world, and occasionally infect human causing mild to severe illness. It is caused by avian influenza A viruses which have been isolated from avian species, particularly waterfowls that are considered the main reservoir for all influenza A virus subtypes. The aim of this study was to detect influenza virus from domestic ducks and geese in different geographical regions(Abu Al-Khasseb, Shatt Al-Arab, Zubair, and Al-Qurnah) of Basrah province, Southern Iraq. The study was conducted on 115 cloacal swabs collected from 60 ducks and 55 geese distributed in different regions of Basrah province. Samples were processed for RT-PCR to amplify matrix (M) gene that is conserved between all influenza A subtypes by using a set of universal primer. We demonstrated that influenza A viruses is prevalent in domestic waterfowls with a significantly higher percentage in ducks than geese; 42 samples (29 ducks and 13geese) were positive to viral M gene. In addition, the virus prevalence was significantly higher in the North of Basrah (Al-Qurnah region) than the other geographical regions. The overall findings of this study obtained a primary picture about the distribution of avian influenza viruses in our region, which is important to prevent the spread of infection to poultry and eventually minimize the risk of acquiring infection to humans. According to our knowledge, this is the first study aimed to identify avian influenza viruses in waterfowls in Basrah province, Southern Iraq.

The purpose of this study is to investigate the in vitro effect of Ho – YAG laser on Complete Blood Picture in blood sample from ureteroscopic lithotripsy ( patients with urinary stones ) . The study is conduct the effect Ho-YAG laser on Complete Blood Count (CBC) was estimated before and after exposure in vitro . The study was conducted on Complete Blood Picture , the taken number of (10) parameters was estimated before and after exposure . Result showed that there was a significant decreased in the percentage of Lymphocyte , PLT , HCT , RBC , HBG , MCHC , after exposure to 2100 nm (Ho-YAG ) laser . It is found that (Ho-YAG ) laser increase some parameters of WBCs , MCV , MONO and MCH and after exposure.

The increasing number of internet users around the world and their demand for multimedia applications like VoIP and video conference force the network providers to think about Quality of Services. Since these new applications are sensitive to network performance, the demand for larger bandwidth is increased especially when network parameters like Jitter, End to End (E2E) delay and Packet loss play a very important role to guarantee such application in terms of quality. In such environment, Quality of Service (QoS) is considered to be the most important network performance parameter which has a significant effect on multimedia applications like Voice Over Internet Protocol (VoIP) and others. internet protocol IPv6 was designed to improve QoS supported by internet protocol IPv4 and to enhance the reliability of packet based network to handle such services. This paper considered three queuing systems which are First In First Out (FIFO), Priority Queuing (PQ) and Weighted Fair Queuing (WFQ) as a comparative study to investigate their effects on real-time applications in IPv4 and IPv6 networks support. The proposed simulation environment was executed by Optimized Network Engineering Tools (OPNET) Modeler 14.5 where the simulation results are collected and analyzed. The results show that IPv6 with PQ provides better performance for real-time applications, despite that IPv6 with WFQ is more efficient with low priority traffic.

This paper aims to study the bifurcation of solution in singularly perturbed ODEs:




the bifurcation of solution in the ODE system will be studied by effect of the system by using Lyapunov Schmidt reduction. Is the study of behaviour of solution of singularly perturbed ODEs when perturbation parameter The bifurcation of solution in this kind of ordinary differential equation was studied in n-dimensional. Sufficient conditions for the system to undergoes (fold,transcritical and pitchfork) bifurcation are given. The ODE will be reduced to an equivalent system by using Lyapunov Schmidt reduction method. Moreover, for this purpose of obtaining curve of the system (Fast-Slow system).

In this manuscript, we apply a new technique, namely local fractional Laplace variational iteration method (LFVITM) on Helmholtz and coupled Helmholtz equations to obtain the analytical approximate solutions. The iteration procedure is based on local fractional derivative operators (LFDOs). This method is the combined of the local fractional Laplace transform (LFLT) and variational iteration method (VIM). The method in general is easy to implement and yields good results. Illustrative examples are included to demonstrate the validity and applicability of the new technique.

Pure Poly methyl methacrylate (PMMA) thin films and PMMA mixed with 1 %, 3%, 5% &7% by weight of ZnS nanoparticles (NP) were prepared by plasma jet technique. Formulation of the polymer with nanoparticles allows for modification of polymer physical properties. The PMMA /ZnS nanocomposite prepared by polymerization in plasma jet and characterized by Ultra Violet Visible UV-Vis, Fourier Transform Infra-Red Spectrophotometer (FTIR), Atomic Force Microscopy (AFM) and SEM to study the effect of ZnS nanoparticles on the optical properties, morphology and structure of the thin films. The FTIR spectra for the films show an interaction between PMMA and NPs. There is a decrease in the optical band gap of films prepared with an increase of the concentration of NPs. AFM and SEM images show that there are a few clusters of ZnS and there is a homogeneous distribution of the nanoparticles in the PMMA matrix.

The study was carried out through a period from June to November 2017 from out-patients and in-patients of Al-Hussein Teaching Hospital in Thi-Qar province South of Iraq. A sum of 132 specimens from the hospital facility condition and different clinical samples of various patients were randomly collected and analyzed for diagnosis of Pseudomonas aeruginosa. These clinical specimens were included wound, burn, ear swabs, urine and sputum specimens. Every single specimen was screened to detect of P. aeruginosa by culturing on suitable media, and 36 isolates of P. aeruginosa were distinguished through biochemical tests and using API 20NE system for confirmation the isolates. All P. aeruginosa isolates were tested against nine antimicrobial discs in vitro. They revealed that having different classes and the results demonstrated that variable resistance to the anti-microbial agents. In attempting to the recognizable proof of P. aeruginosa having the DNA, Polymerase chain reaction (PCR) was utilized in light of particular groundwork for 16S rRNA genetic marker. The results demonstrated that PCR has observed to be quick and sensitive and particular for recognizable proof of P. aeruginosa. Furthermore, 16S rRNA was utilized as confirmation feature, while lasB detected as virulence gene also to utilize as pathogenesis quality of the bacteria.