Basrah Journal of Veterinary Research

In order to estimate the hepato-protective activity of Vit.B12 in diazinon hepatotoxic effect, the current study was performed on twenty-four male adult Wistar rats. They’d divided into four equal groups for 30 days: group A (control group) served as control; group B (Dzn group) administered an oral daily dose of 1/10 LD50 (3.8mg/ kg.bw) from diazinon; group C (Dzn + Vit.B12 group) administered an oral daily dose of 1/10 LD50 (3.8 mg / kg bw) from diazinon, in addition to the systemic intramuscular doses of (4 mg/kg) from Vit.B12 in daily basis; group D (Vit.B12 group) administered daily systemic intramuscular doses of (4 mg/kg) Vit.B12 only. The results showed that group C had a significant improvement in the antioxidant indicators compared with group B in which the CAT, GSH, and SOD serum activities showed significant increasing (P≤0.05) in group C, in addition, it revealed a significant decreasing (P≤0.05) in the values of MDA and peroxynitrite comparing to the same levels of the group B. The histological results of the liver of group C showed a great improvement compared with group B which showed normal hepatic architecture consisting of normal hepatocytes, and normal sinusoids with normal other hepatic components except mild central vein dilation. The current study concludes that vitamin B12 helps to ameliorate the diazinon hepatotoxic effects as a model for a wide spread of organophosphorus compounds in agriculture and veterinary sectors by ameliorating its harmful oxidation on the liver since vitamin B12 is cheap and available as a commercial supplement therefore we recommend its uses

Nineveh is one of the main governorates of Iraq for sheep ranching. However, in recent years, E.coli has caused many problems and diseases in sheep. Therefore, the current study was conducted to isolate E.coli from sheep and their surrounding environment in two types of breeding systems (open and closed) and to study the effect of the breeding system on the rates of its isolation. During the period from 1 Feb to 30 Apr 2022, 380 samples from milk, skin swabs, oral swabs from lambs, water, dry fodder, feces, bedding and green grass were collected. Eight flocks of sheep classified into two groups open and closed breeding system. Standard microbiological methods were done using Eosin Methylene Blue (EMB), MacConkey and Brilliance agars cultures. Then, the isolates were confirmed using biochemical tests. Molecular confirmation of E.coli was performed using specific primers uidA gene. The current study showed that out of 240 samples, 130 samples from open breeding system at a rate of 54.2% and out of 140 samples, 90 samples from closed breeding system at a rate of 64.3%, were positive for the bacterial isolation and PCR assay. The high isolation rate of E.coli from the sheep closed breeding system, indicates the lack of hygiene procedures application with regard to flocks health, as well as the sanitary procedures followed in animal housing. Close monitoring of food animal production is essential and that it could reduce the potential public health risks in both animal and human medicines.

The study was conducted to investigate the Seroprevalence of Glanders in south part or Iraq. Eight hundred (800) local and different horse breeds aged from (1.8–25) years old and from both sexes was examine during the period started from a15.oct. 2020 to 20 April 2021. The 184 serum samples were drained from suspected horses to examined by using double antigen multispecies Eliza of Burkholderia mallei. Results show that only 4 (2.17%) horses give a positive result with the Elisa test in Maysan governorate. Whereas negative horses for glanders included 180 (97.82%) in the south of Iraq, and that Basrah and Thi-Qar governorates were negatively against glanders. Such favorable result help for equestrian regions and horse breeding.
It has been concluded that, although a very low infection rate was indicated in maysan governorate, However, Glanders could be re-emerging compared with other south governorates (Basrah and Thi-Qar) which being free. Moreover, Basrah and Thi-Qar governorates can be used as free areas.

The present research was designed to evaluate the protective effect of crude alkaloid extract of Matricaria chamomilla L. (MC) against pentylenetetrazole (PTZ) induced convulsions in chicks. A total of seventy-two chicks were randomly divided into six groups each one consisting of 12 chicks. The first group was considered to be a negative group that received subcutaneous injection of normal saline, the second group (positive control) received subcutaneous injection of PTZ (90 mg/kg), and the third group received sodium valproate (200 mg/kg) orally for six days. The other groups (fourth, fifth and sixth) were treated with single intraperitoneal injection of crude alkaloids extract of MC (20, 40, 80 mg/kg) respectively. After 30 minutes of the treatment, the groups (third to sixth) received PTZ and observed the convulsion signs for the next 30 minutes. At the end of the experiment, various biochemical parameters were assessed including, brain gamma-aminobutyric acid (GABA) and glutamate, serum catalase (CAT), glutathione reductase (GR), malondialdehyde (MDA), and 8-isoprostane, and some electrolytes (potassium K+, sodium Na+, chloride Cl-, ionized calcium iCa2+, total calcium TCa2+), pH, and glucose levels in serum. The results indicated that the group treated with crude alkaloids extract at higher dose (80 mg/kg) showed a significant increase in CAT and significantly decreased in 8-isoprostane, and an increase in Na+ ions concentration in serum. It was concluded that pretreatment of crude alkaloids extract (80 mg/kg) of MC offers anticonvulsant activity by reducing the mortality rate induced by PTZ, as well as it plays an important role in inhibiting oxidative stress.

A common characteristic of Mycoplasma diseases is their chronicity, Mycoplasma
spp. infection have been frequently associated with upper respiratory infections, chronic lung
disease , asthma, meningeal encephalitis, mastitis, arthritis, heart problems, sterility, bone
problems , oviduct dysfunctions and death in human and different animal species. In former
years, changeable expression of membrane antigens has been detected in a number of
Mycoplasma spp., resulting in the assumption that immune prevarication may be an important
part of the their infection and pathogenesis. It has been determined how the attachment
organelle, which mediates the complicated interactions between various adhesins and auxiliary
adhesion proteins to mediate the critical first stage of cytoadherence to respiratory tract
epithelium. Additionally, it has been demonstrated that inflammatory cytokines cause tissue
damage by intracellular localization, direct cytotoxicity, and activation of the inflammatory
cascade via Toll-like receptors (TLRs), and inflammosome activation, which causes air passage
inflammation. All of these play crucial roles in the infectious process. This paper seeks to
provide a thorough assessment of recent developments in our understanding of Mycoplasma
pathogenesis with the understanding of its virulence mechanisms. A common characteristic of Mycoplasma diseases is their chronicity, Mycoplasma
spp. infection have been frequently associated with upper respiratory infections, chronic lung
disease , asthma, meningeal encephalitis, mastitis, arthritis, heart problems, sterility, bone
problems , oviduct dysfunctions and death in human and different animal species. In former
years, changeable expression of membrane antigens has been detected in a number of
Mycoplasma spp., resulting in the assumption that immune prevarication may be an important
part of the their infection and pathogenesis. It has been determined how the attachment
organelle, which mediates the complicated interactions between various adhesins and auxiliary
adhesion proteins to mediate the critical first stage of cytoadherence to respiratory tract
epithelium. Additionally, it has been demonstrated that inflammatory cytokines cause tissue
damage by intracellular localization, direct cytotoxicity, and activation of the inflammatory
cascade via Toll-like receptors (TLRs), and inflammosome activation, which causes air passage
inflammation. All of these play crucial roles in the infectious process. This paper seeks to
provide a thorough assessment of recent developments in our understanding of Mycoplasma
pathogenesis with the understanding of its virulence mechanisms.

Rosmarinus officinalis, L. (Lamiaceae) offers medicinal potential against hepatotoxicity and nephrotoxicity Due to its antioxidant and anti-toxicity properties. The objective, of this research is to explore into the ethanol extract of Rosmarinus officianalis leaves and its efficacy against cypremethrin induce toxicity in The liver and The kidney of male rabbits. Forty male rabbits were employed in this experience, group one ( without exposure); rabbits received corn oil ( 1 ml) group two; rabbits oral exposure to cypermethrin with dose (66.5 mg/kg/b.w ( 1/10 of LD50) dissolved in corn oil , group three( protective group) received rosemary extract with dose 100mg/kg plus cypermethrine with dose 66.5 mg/kg/b.w., group four received rosemary extract with dose 200mg/kg plus cypermethrine with dose 66.5 mg/kg/b.w. the above doses once per day for 6 weeks respectively.In accordance with the current study's findings, liver enzyme levels significantly increased. ALT,ALSand ALP),Lipid profile (TC,TG.LDL-cand VLDL_c) and kidney function tests (urea, a creatinine, uric acid and blood urea, nitrogen concentrations ) but significant decrease in (Total protein and HDL_ c value ) in the treated group with cypermethrin , .whereas the animals received rosemary extract in both dose plus cypermethrin produced notable decrease in the levels of the vital parameters of the liver and kidneys are with the normal level. : the current study displayed that the Rosmarinus officinalis has antitoxicity activities as seen via decreasing liver enzymes, lipid profile and kidney function tests is accompanied by elevated the levels of total protein .

Grape seed extraction has many beneficial effects on the cardiovascular system and blood pressure and has also been proven to have anti-obesity effects and/or improve fatty acid oxidation and insulin responses. The objective of this study is to evaluate the effectiveness of grape seed extract on some physiological parameters in multiple systems. To this end, male mice were injected intraperitoneally with (100 mg/kg B.W) for 7 days. Serum was collected to detect the concentration of ALT and TSB (liver functions). Urea and creatinine (a kidney function) concentration was measured. Testosterone, LH, and FSH (male reproductive function) concentration were evaluated. Our results revealed a significant reduction in ALT concentration in the grape seed extraction group compared with the control group. Total serum bilirubin was non-detectable in both the grape seed extraction group as well as the control group. Urea and creatinine concentrations appeared to have no significant change between both grape seed extraction and the control group. Testosterone, LH, and FSH hormone levels were significantly raised in the grape seed extraction group compared with the control group. Prolactin hormone results revealed no change in both the grape seed extraction group and the control group. Our study showed up that grape seed extract can improve male reproductive hormones without any toxic effect on the liver or kidney.

Fasciolosis is disease caused by a liver worm called Fasciola is one of the dangerous parasites that affects the livestock specially sheep are wide spread in many countries of the world including some Arab countries. This worm attacks animals such as cows, buffaloes, camels, horses, donkeys, sheep, goats and rabbits that feed on grass and plants. The worms inhabit the bile ducts in the liver of humans and animals and they multiply and spread in all tissues causing many damages and risks to public health and great losses in animal production Therefore we will present the detailed aspects of this disease its classification and description of species symptoms methods of infection life cycle, epidemiology pathological diagnosis of prevention