Basrah Journal of Veterinary Research

The current study was undertaken to investigate the possible protective
effect of Ocimum basilicum seeds oil and Linum usitatissimum seeds oil on some
physiological parameter and histopathological changes when induced acute
myocardial infarction in rabbits by isoproterenol.Thirty-six male rabbits were divided
into six groups: group (C): control negative,group (ISO): received isoproterenol (control
positive), (BP) group: basil seed oil protective group, (FP) group: flaxseed oilprotective
group, (BT) group: basil seed oil treated group and (FT)group: flax seed oil treated group.
The results showed thata significant decrease in the biomarker enzymes(CK-MB, LDH,
AST, ALT and ALP) concentration and serum MDA in the BP, FP, BT, and FT groups
compare with ISO group. In addition to that, the basil oil and flaxseed oil protect and
improvement histopathological findings in the myocardium tissue of isoproterenol effect.

The functions of the ovaries are controlled by many exogenous and endogenous
factors, including changes in the biochemical and endocrine glands that occur in the
follicular fluid during the breeding season in the bitches. The aim of this study was to
determine and compare the concentrations some hormonal in the peripheral
circulation and follicular fluid of bitches during the breeding season. For this
purpose, ovaries collected from adult bitches immediately after ovariectomy, and
blood samples were also collected from these bitches before and after season. The
follicular fluid and blood serum samples were analyzed for hormonal concentrations
using commercial kits.
The results showed that the concentrations of estrogen, progesterone and
testosterone in blood serum at season (54.31±0.49, 20.75 ±0.12, 0.75±0.05)
respectively. While the concentrations of estrogen, progesterone and testosterone in
blood serum at out season (10.88±0.39, 0. 61±0.036, 0.063±0.042) respectively. The
concentrations of estrogen, progesterone and testosterone in follicular fluid at season
(69.9±0.44, 28.46±0.82, 0.331±0.65) respectively. While the concentrations of
estrogen, progesterone and testosterone in follicular fluid out season (there are no
found ovarian follicles).
The present study a significantly higher (P< 0.05) in blood serum and follicular fluid
at season than that out season blood serum.

This study was undertaken to isolate alkaloids from Haloxylon Salicornicum plant to
show the curative effect after short-term daily orall administration for 10 days (300 mg/Kg
B.W.) on gastric ulcer,hematological parameters and some biochemical parameters in
gastric ulceration rabbits induced by indomethacin.Moreover,some qualitative chemical
analysis,thin layer chromatography,UVspectroscopy and FTIR spectroscopy were used to
identify alkaloid compound .Rabbits were divided randomly into three groups,6 rabbits in
each.Group one received 3 ml normal saline orally (control group).Group two was treated
with indomethacin (75 mg/Kg B.W.) (gastric ulceration group).Third group gastric
ulceration rabbits post-treated with isolated alkaloid (300 mg/Kg B.W.).The results of the
present study indicated that isolated alkaloid has Rf= 0.86,high significant decreased
(p≤0.001) in RBC,Hb,PCVand mucin,(P ≤ 0.01) in MCHC and PH,no significant
difference (P ≥ 0.05) in MCH in gastric ulceration rabbits compared with control group
and observed high significant increased (p≤0.001) in MCV, MDA and gastric juce
volume,WBCcount appeared high significant increased (P≤0.01).While, observed high
significant increased(P≤ 0.001) in Hb,PCVand mucin,(P≤ 0.01) in RBC,MCHC and
pH.Significant increased(P≤0.05) in MCH after treated with isolated alkaloid and high
significantly decrease (P ≤ 0.001)in MCV,gastric juce volume and MDA and significant decrease (p≤0.05) inWBC count compared with gastric ulceration group
.This study concluded that isolated alkaloid from Haloxylon Salicoricum plant can be used
to treat gastric ulcer effectively

This research was already prescribed for the identification and serotyping of
Salmonella isolates from 205 samples totally different, including frozen chicken
meat(thigh, wings, liver, 40 samples for each one), 40 samples of frozen beef meat 4
and fresh beef meat ( liver, muscle, and ground beef , 15 each ) in Basrah throughout
the amount between 4th October 2017 to 27th February2018. Results showed that the
overall rate of Salmonella isolates were 22.4 percent by conventional victimization
isolation of Xylose Lysine Deoxycholate Agar (XLD).The highest percentagewas
appeared in liver of imported frozen chicken meat (80%), while the lowest percentage
was in liver of local fresh beef (6.6%). The results of identification of Salmonella by
conventional biochemical test and API 20 E system were76.0% and 84% ,
respectively, while the results of PCR technique by using 16srRNA was( 20/20)100%
. The result of serotyping on Salmonellaisolates revealed that serotype
SalmonellaTyphimurium (40%), whereas the lower number was Salmonella serotype
Kentucky (15%).

One hundred fifty cheese samples were collected between 8 October 2017 to
February 2018. Fifty samples from each cows , buffalos and sheep. The sample
transferred to TSB (tryptone soy broth) and PBS (Phosphate buffer saline) for
enrichment and cooled enrichment procedure respectively. Using Yersinia selective
agar TSB enrichment showed high percentage of suspected Yersinia isolation. Eleven
isolates from cow cheese (22%), 10 isolates from buffaloes cheese (20%) and 8
isolates from sheep cheese (12%). In contrast PBS enrichment showed better
selectivity to reduce bacterial number other than suspected Yersinia enterocolitica
isolates. The results indicate there were 8 isolates from cow cheese (22%), 9 isolates
from buffaloes cheese (20%) and 7 isolates from sheep cheese (16 %). The suspected
colonies that grow on selective agar and having bull eye appearance were subjected to
biochemical identification. The results showed that cow and buffaloes cheese were
contaminated with this bacterium at the percentage of 8% and 6% respectively. Sheep
cheese were also contaminated with Yersinia enterocolitica at the percentage of 4% .
The total percentage of isolation of Yersinia enterocolitica from all animals were
6.%.. The isolated strains were highly susceptible toward azithromycin, streptomycin,
and Gentamycin, followed by Ciprofloxacin and Chloramphenicol (93.3%). The low
susceptibility were found toward vancomycin (6.66%) followed by Cloxacillin
(33.3%). The result of polymerase chain reaction (PCR) for enterotoxin genes, ystA
and ystB were investigated by PCR using a pair of primers for each. The results showed that ystA gene was absent in all nine investigated strains while ystB gene was
present in four strain at a ratio 44.4%.