Muthanna Journal of Pure Science (MJPS)

Binding of 35[S]- methionine-labeled Helicobacter pylori to tissue culture
was carried out using human colon carcinoma epithelial cells (Caco-2),
and human adenocarcinoma tissue culture cells (AGS). In this research,
clinical isolates (smooth) as well as type culture collection (rough) strains
were used in binding and inhibition of the binding study of H. pylori to
tissue cultures using monoclonal antibodies (MAbs) raised against
bacterial lipopolysaccharides (LPS). The task of the work was to
discriminate and evaluate the role of LPS as bacterial adhesin in the
binding as well as the specificity of the binding of various H. pylori strains
to cell lines. Binding of H. pylori, clinical isolate Hel 305(smooth
strain),to Caco-2 cells was strongly inhibitedby species-specific MAbs
raised against Hel 305-LPS antigen. It was also recognized that inhibition
of binding of Hel 305 strain to Caco-2 cells was high effective when using
species-specific MAbs in comparison with MAbs raised against LPS from
type culture collection CCUG 17874 (rough strain). In the same manner,
binding of rough CCUG 17874 strain to Caco-2 cell line was inhibited by
species-specific LPS MAbs stronger than with MAbs raised against Hel
305 LPS. In both overlay cases, no significant inhibition with other LPS
MAbs of different origins was detected, after bacterial incubation with
MAbs raised against LPSs from other tested H. pylori strains.

A series of novel Schiff bases have been prepared from the condensation
reaction of benzil with 4- chloro aniline compound , the product have been
treated with many derivatives of( 2-, 3-, 4-, 2,3-, 3,4-fluoro) phenyl boronic
acids by Suzuki coupling reaction to give new schiff bases.
These compounds have been characterized by spectroscopic methods (FTIR,
UV-VIS.), TLC and melting point.

Someanatomical remarks of male reproductive system of adult
perdix, histological structure of testes of perdix and the sequence
events of spermatogenesis and spermiogenesis are dealed in this
research. The anatomical study of the male reproductive system
in perdix include: the paired testes which remain within the
abdominal cavity and connect with the dorsal wall via
mesorchium. The testes are covered by a thin tunica albuginea.
The spermatic cord, tunica vaginalis, and scrotum are lacking. the
seminiferous tubule has seminiferous epithelium; spermatogenic
cells and sertoli cells lie on the basement membrane and that
whole is circumscribed by interstitial tissue which contain leydig
cells. The sequence events of spermatogenesis and
spermiogenesis in seminiferous tubule of adult perdix start with
spermatogonia. Three types of spermatogonia are present which
include: A-spermatogonia, B-spermatogonia and Intermediate
spermatogonia. The B-spermatogonia give rise to primary
spermatocytes. The primary spermatocytes appear six different
phases. Which are include: (preleptotene, leptotene, zygotene,
pachytene, diplotene,anddiakinesis phases ). The secondary
spermatocytes being short - living cells and are derived from the
primary spermatocytes. With the appearance of spermatids,
spermatocytogenesis end. The spermatids undergo complex
processes of differentiation depending on changes in the
acrosome and nuclei. These changes show 17 phases lead to form
sperms.
The sertoli cells possess elongated nuclei which are often
triangular in shape. The sertoli cells form an extensive
ramification around and between the germinal cells making
intimate contact with them. A cycle of stages in sertoli cells
structure are not identified.
The leydig cells are scattered singly or in small groups and are
chiefly found in the larger intertubular space. The basement
membrane of convoluted seminiferous tubule is comprised of a
layer of connective tissue and myoepithelial cells. The myoid cells
are elongated cells, with oval nuclei, surrounded by a small
amount of cytoplasm.

Hydatidosis is a parasitic disease caused by infection
with larval stage of cestode worm Echinococcus
granulosus ,it is a worle_wide disease results in serious
health and financial loss. Iraq considered as an endemic
region for this disease. the association of HLA –alleles
frequency with the occurrence of various diseases were
studied . and significant associations were found, no
sufficient studies on hydatidosis and possible role of HLA
typing and the immune status of the patients and
association of them with cyst characteristics ,and
cytokines profile and their effect on production of
immunoglobulin . blood samples were collected for
extraction of DNA and amplification by (PCR-SSO) and
HLA typing by Automatic Line Probe Assay (Auto-Lipa) for
HLA typing of both patients and healthy control groups, to
study the association of certain HLA alleles. With
occurrence of Hydatid disease. The present study
reported increase in frequency of HLA-A*0273 in hydatid
patients compare to healthy control .

A set of orthonormal polynomials, which represent scalar
functions, are given in this paper that represent balanced basis
over an area of annular pupil. These functions are used to
represent the aberration of imaging systems with annular
pupils. Another orthonormal set of polynomials related to those,
which represent vector quantities, can be generated from the
gradients of annular Zernike polynomials (ZP), and the
orthogonality is made by MATLAB code for Gram Schmidt
orthogonalization method (GSOM). A relation of these
polynomials to the circular ZP and circular ZP gradients are
represented also in this work.

A new chelate complexes of Zn(II),Cd(II) and Hg(II), have been prepared by
reacting these ions with the ligand 1,3-Bis (4,5-diphenyl imidazole azo) benzene
(BABI),The preparation of the complexes have been conducted after fixing
the optimum conditions such as pH and concentration .UV- visible spectra of
these complexes solution have been studied for a range of pH and concentration
which obey Lampert-Beers Law .The structure of complexes are deduced
according to mole ratio method which were obtained from the spectroscopic
studies of the complexes solution .The ratios of metal: ligand obtained are (1:1)
for these complexes . (UV-Vis) absorption spectra of ethanolic solution of
complexes showed bathchromic shift , as compared with that of free ligand .The
infrared spectra of the chelating complexes have been studied , Depending on
these results the coordination between our ligand and the metal ions take place.
The conductivity measurements , elemental analysis ,the percentage of metal
ions was determined .Depending on these results ,we may conclude that the
ligand was tetradentate . Also the proposed geometrical structure of the
complexes of Zn(II),Cd(II) and Hg(II) ions are octahedral. .Semi-empirical
methods (ZINDO/1, PM3 and ZINDO/S) were carried out by using hyper
chem.program to evaluate heat of formation ΔH˚f, binding energy ΔEb, dipole
moment, for all complexes , also the vibration frequencies, and electronic
transitions was calculated for prepared ligand(BABI)..

Buried structures of Babylonian houses district are investigated using 2D
resistivity imaging techniques . 2D resistivity imaging results show upper
zone , near the surface with a high resistivity value which can often be found
throughout the whole area and is characterized by dried clayed and sandy
soil with broken and weathered different archaeological materials such as
broken brick and slag mixed with core boulders. Underneath this upper zone,
there is a prominent low resistive layer probably caused by the moisture in
this region that reduces the resistivity with thickness which differs from
parts of the site to the others . Also, 2D resistivity images illustrate zone
with a high resistivity value indicating more intact zone of archaeological
structures down to the bottom of the image . It indicates an archaeological
structure extending vertically through the images which is typical for
archaeological clay brick walls . 2D results show presence of several
anomalies at different depths ranging from a very high to a low resistive
anomaly. The interpretation of these anomalies , ranged from core boulders,
voids , air or clay –filled cavity or grave based on the geophysical and
archaeological data of the site .

Present study was conducted at Dialysis unit in Al-Sadder
medical city /Al-najaf Al-Ashraf during the period from July
till November, 2012,wich aims to determined the effect of
chronic renal failure on gonadal hormone in male by using
70 male subjects divided into two groups 50 of them had
chronic renal failure and the remaining 20 subjects were
normal healthy individuals served as a control .
The result of this study revealed that significant increased
p<0.05 in serum FSH 30.651 ± 0.12 (U/L), renal failure
patients compared with healthy group 9.363± 0.65 (U/L).
and significant increased p<0.05 of serum LH
concentration in renal failure patients 17.452± 0.22(U/L)
as compare with HT group 6.185±0.43(U/L). also the study
showed significant increase p<0.05 of serum PRL
concentration in renal failure patients 29.779 ± 0.58(Ug/L)
as compare with HT group 8.188± 0.33

Wild type E. coli DS 17 is a clinical isolate associated with
epidemic UTI was studied. This isolate recognized selectively
well Galα1-4Gal sequence of globoseries glycolipids
separated on thin-layer chromatogram (TLC), coated in
microtiter wells and incorporated into liposomes. E.coli DS17,
representing a class II adhesin, showed high affinity to the
disaccharide Galα1-4Gal sequence of the globoside
glycolipid receptor in all assay systems. Weak recognition of
the epitope, Galα1-4Gal, in the terminal position of
globotriaosylceramide (Galα1-4Galβ4Glcβ1Cer) and also
weak binding of DS17 to the disaccharide sequence within
Forssman glycolipid GalNAcα3GalNAcβ3Galα1-
4Galβ4Glcβ1Cer. This strain failed to recognize glycolipids
missing the disaccharide sequence like
gangliotetraosylceramide Galβ3GalNAcβ4Galβ4Glcβ1Cer.
The modified E. coli DS 17 by replacing class II adhesin with
class III (PrsG) type, (DS17-1), bound Galα1-4Gal sequence of
globoside less than Forssman glycolipid. E. coli DS17-8
strain, DS17 with the knocked out adhesin, revealed no
binding to the glycolipids in all assay system. Using
liposomes aggregation assay, E. coli J96, representing class I
adhesin, showed strong recognition of globoside
incorporated into liposomes, weakly when Forssman
glycolipid was used and very weak binding to
globotriaosylceramide was detected.
Introduction

In this study, two novel azo compounds were synthesized by diazonation of 4-
aminoantipyran followed by coupling with 4-(dimethylamino)benzaldehyde and N-
(4-hydroxyphenyl)acetamide. The synthesis azo compounds were characterized by
IR, CHN, UV-Vis., TLC and melting points. Also, was carried out to determine the
antibacterial activity of these compounds against burn infection isolated bacteria.
Thirty samples were collected from patients related to Samawa-teaching hospital
burn unit. Sixty five isolate from both sex males (53.33%) and females (46.66%).
Staphylococcus aureus was found to be must common isolated (43.07%), then
Pseudomonas aeuroginosa (21.53%), Klebsiella spp. (12.30%), E. coli (9.23%),
Proteus mirabilis (7.69%), Streptococcus pyogenes (4.61%) and Aeromonas
hydrophila (1.53%) . The synthesis azo compounds showed a good antibacterial
activity against the isolated bacteria by using disk diffusion method. MICs values
were determined for all types in broth modulation assay.

A new , simple , sensitive and rapid spectrophotometric method is
proposed for the determination of trace amount of cobalt (II) . The method is
based on the formation of a 2:1 complex with 3-(4′- antipyriyl azo) 1- nitroso-2-
naphthol (APAN) as a new reagent is developed . The complex has a maximum
absorption at 432 nm and εmax of 3.3 X 104 L. mol-1. cm-1 . A linear
correlation (0.1 – 2.5 μg. ml-1) was found between absorbance at λmax and
concentration . The accuracy and reproducibility of the determination method
for various known amounts of cobalt (II) were tested . The results obtained are
both precise (RSD was better than 0.60 % ) and accurate (relative error was
better than 0.2 % ) . The effect of diverse ions on the determination of cobalt
(II) to investigate the selectivity of the method were also studied . The stability
constant of the product was 1.0 X 108 L. mol-1 . The proposed method was
successfully applied to the analysis of synthetic mixtures and raw milk without
any preliminary concentration or separation.

The objective of this study was to assess the levels of lead
(Pb) in the human milk samples of healthy lactating
women who were living in Al- Muthanna province in Iraq
and to investigate the effect of region (urban and rural)
and mother’s age on the Pb concentration. Pb levels were
measured by flame atomic absorption spectrometry. The
total samples were 70 milk samples at different stages of
lactation) from 5 days to 56 weeks postpartum). Pb levels
of milk samples were found to be (6 .81 ± 1.31) μg/dl. The
concentrations of Pb in human milk of lactating women in
urban areas were higher than rural areas. There was a
positive correlation between the concentration of lead in
milk samples and mother’s age (P < 0.05). Considering
the high level of Pb in breast milk in this study, it is
important to identify the possible sources of this heavy
metal in maternal environment.

In this study we used two doses of indomethacin drug
for two cases mild and sever of oligospermia in men
Twenty patients were suffering for long time of
infertility, for this reason sperm concentration was 1 ×
106 or less . The patients divided into two groups.
The first group received 25 mg/twice a day. and the
second group received 25 mg/three time a day for every
cases mild and sever.
Blood sample in all cases were taken for FSH LH and
T(Testosterone). assay before treatment (base line level)
as well semen sample were taken from each patients for
analysis.
After 30,40,50 and 60 days of indomethacin treatment
were showed a significant differences for gonadotrphin
hormones and sperm concentration as well after
treatment pregnancies were happened for patients
sufered from infertility.

The potency of silymarin in ameliorating the lipid peroxidation and antioxidant
status has been evaluated in streptozotocin-induced diabetic rats. After induction
of diabetes, by single injection of streptozotocin (60 mg/ kg bw), ninety six (48
intact and 48 diabetic) mature male rats were assigned to 4 equal groups, treated
for three periods (15, 25, and 35 days) by drenching intact and diabetic rats with
drinking water (C and D groups) and drenching intact and diabetic rats with 200
mg/kg bw of silymarin suspension (SD and S groups). Body weights has been
registered at five days interval. After the last day of each period, 8 males from
each group were anaesthetized, sacrificed, and blood samples were obtained for
assessment of serum lipid profile (total cholesterol; T-c, triglycerides; TG, high
density lipoprotein; HDL-c, low density lipoprotein; LDL-c, and very low
density lipoprotein; VLDL-c), liver function test (ALT, AST, and ALP
concentrations), and oxidant- antioxidants activity ( superoxide dismutase; SOD,
catalase; CAT, total glutathione; GSH, glutathione reductase; GSH-r, glutathione
transferase; GTH-t, glutathione peroxidase; GSH-px and malondialdehyde;
MDA). Body weight showed marked increase in control and silymarin treated
male rats compared with diabetic control in all periods of experiment. Serum ALT, AST, ALP, T-c, LDL-c, and VLDL-c concentrations decreased
significantly in C, DS, and S groups compared with diabetic control (D group) at
the end of each period of the experiment, whereas TG and HDL concentration
showed significant increase at the same periods. At the end of each period of the
experiment, serum oxidant-antioxidant status revealed significant decrease of
serum MDA, SOD, CAT, GSH-t, GSH-r and GSH-px activity, whereas total
GSH activity was significantly increased. It can be concluded that 200 mg/kg
b.w. of silymarin drenching for 15, 25, and 35 days perform positive role in
ameliorating the different complications of oxidative stress produced by diabetes
mellitus.

Anti-tuberculosis drugs susceptibility were conducted among the new
case, the findings showed that 18 isolates were resisted to five first drugs
treatment (Isoniazid, Rifampicin, Ethambutol, Streptomycin and
Pyrazinamide) among 131 isolates with different resistance patterns that
monoresistance was shown in 12 (9.2%), 2 drugs resistance in 3 (2.3 %),
3 drugs in 2 (1.5 %) and 4 drugs in 1(0.76) patients. The MDR was found
in 6 (4.6 %) patients. *This work is part from PhD thesis

We tried to review the registered annual cancer cases in
Al-Muthana and evaluate the environmental influences on
our results.

We introduce the definitions of uncertain measure and convergence in
measure , also we proved some properties of convergence in measure for
uncertain sequence.

The work consists of two parts . The first including of
photodegradation of Methylene Blue by using advanced oxidation
processes at lab temperature in presence and absence of UV light . The
second part consists of photocatalytic reaction of Methylene Blue has
been carried out with sun light on ZnO powder. Advanced oxidation
processes have proved to be highly effective in degradation of most
pollutants in wastewaters. The photocatalytic bleaching of the dye was
observed Spectrophotometer . The aim of this study was to show the
some parameters such effect of initial dye concentration, pH , initial
hydrogen peroxide dosage and catalyst ZnO on the oxidation of MB dye
. Generally it has been found that the rates of photodegradation of MB
dye was higher in presence of UV radiation . Also, photocatalytic ZnO
showed the importance with sun light lead to enhance the generation of
hydroxyl radicals which drive the photooxidation processes. It has been
found the photodegradation reaction of dye under UV obeys the first
order kinetics. The work was done on the roof of the laboratory building
and the time of irradiation was between 1.00 am - 3.00 pm and the date
of experiments was in July .

A two types of bridged complexes of Iron (III) with two types
of ligands phthalocynine & tetrapyrazino Porphrazine were
prepared according to adjusted procedure as in the litratures to
forms a two complexes , Phthalocyanina to separately Iron(III)
chloride[I] and tetrapyrazino Porphrazine iron (III) chloride[II]
were converted to phthalocyanato Iron(III) hydroxide [III] and
tetrapyrazino Porphrazine Iron(III) hydroxide[IV] separately. The
hydroxide complexes then converted to the bridged polymeric
fluoride complexes [V] and [VI] .The copolymer [VII] were
prepared from the mixed of hydroxide complexes [III] and [IV]
of(1:1) ratio to the polymeric fluoride of two ligands .The
compounds then characterized by elemental analysis and
spectroscopic methods.

The house mice kidneys lay on each side of the upper
lumbar vertebra in the abdominal cavity behind the
peritoneum. The right kidney was situated more cranially
than the left. The shape of two kidneyswas bean like
structure. The mean weight of the right kidney was
0.076±0.013gm while, the left kidney was
0.072±0.011gm. The mean length, width and thickness of
the right kidneys were 7.4±0.647mm, 5.098±0.427mm,
3.624±0.198mm respectively while those of the left were
6.8±0.518mm, 4.768±0.481mm,
3.468±0.123mmrespectively. The kidneys consist of two
distinct regions, the outer cortex is reddish brown in color
and inner medulla, and both terminate bysingle elongated
renal papillae. The house mice kidneys were covered by a
thin dense connective tissue capsule with adipose tissue.
The nephron composed of large and small rounded or
irregular renal corpuscle; each The renal corpuscle is
forms the glomeruli that enveloped by Bowman’s capsule,
and the proximal convoluted tubules, loop of Henle and
the distal convoluted tubules. The cortex consists from
renal corpuscles, proximal convoluted tubules and distal
convoluted tubules. The proximal convoluted tubule arises
from the parietal epithelium of Bowman’s capsule of the
urinary pole of the renal corpuscle lined by simple high
cuboidal epithelium. The distal convoluted tubules were
lined by simple low cuboidal epithelium. The part of the
distal convoluted tubule in contact with glomerular root
has the macula densa. The medulla of each kidney is
formed from thick parts lined bysimple cuboidal
epithelium and thin parts of the loops of Henle lined by
simple squamous epithelium and the thin limb had a
distinct rounded lumen and collecting tubules are lined by
pale cuboidal epithelium with rounded nuclei and
MJPS….Vol.(2)….No(1)….December 2014
basophilic cytoplasm. The terminal portion of these
tubules is lined by columnar epithelium and is called
papillary ducts.

Fifty samples of adults German cockroach Blattella germanica were collected
from different parts of Al-Diwaniya city during the period between October
2013 until March 2014 in order to isolate and identify the parasitic pathogens
from external and digestive tract of German cockroach.
78% of the examined cockroaches were infected with one or more species of
protozoa or worms, the infected percentage of protozoa was 62% while the
percentage of infection by worms was 70%. Prevalence of Endoparasites was
statistically higher significantly than the parasites carried on the external surface
by using Chi square test at (P< 0.05).
Eight species of parasites were isolated from the external surface and digestive
tract of German cockroaches included two species of protozoa were
Nyctotherus ovalis and Entamoeba coli cyst by infection present of (62 and
4)%, respectively; and four species of worms Hammerschmiditella diesingi,
Thelastoma bulhoesi, Blattellicola blattae and Gordius robustus (42, 28, 16 and
14)%, respectively, in addition to the eggs of worms Enterobius vermicularis
and Ascaris lumbricoids by infection present of (6 and 4)%, respectively.
The results showed presence of three species of parasites shared between
humans and animals which carried by German cockroach were Entamoeba coli
cyst, Enterobius vermicularis eggs and Ascaris lumbricoids eggs which were
isolated from the external body of German cockroach.

The aim of this study is to investigate the temperature
dependence of semiconductor band gaps in low
dimensional semiconductor heterostructures. Low
dimensional semiconductor systems are considered as a
foundation of modern quantum devices. In this study, the
temperature characterization of Ga1-xAIxAs/GaAs quantum
well systems was determined by using Varshni relation.
The band gap of the investigated semiconductors were
observed at deffirent temperatures. The energy bandgap
of semiconductors tends to decrease as the temperature is
increased.

Ferrites are ceramic compound with Iron oxide as there principle
component. Hexagonal ferrites are commonly used in microwave
absorption applications. Hexagonal barium cobalt oxide prepared
by traditional ceramic method in both of micro and nanosacles
then the characterization of the prepared powders were done by
using Scanning Electron Microscopy (SEM) and X-ray diffraction
(XRD). From X-ray diffraction pattern results of prepared powders
it’s found that the nano compound need higher temperature to
form desired ferrite (nano BaCo2Fe12O27). The XRD results shows
that the nano barium cobalt hexaferrite has more noise peaks.
The SEM images shows that the hexagonal shape crystals appears
more clearly in micro barium cobalt hexaferrite.

In this paper , we have discussed transistors made from single
molecules in which one silver ion is connected to gold electrodes by
organic barriers. By tuning the length of the organic barrier we are
able to control the coupling between the ion and the electrodes. For
relatively long linker molecules, giving weak coupling, the molecule
functions as a quantum dot . This work shows that the properties of
a molecular transistor can be controlled by the physical properties
of the molecule .The ability to design the electronic states of a
molecular device using(SIESTA and Smeagol) , together with the
ability to measure individual molecules, will play an important role
in molecular electronics and in the physics of nanometer-scale
systems . This paper is also to study electron transport in
nanoscale objects, especially the devices made from single
molecules . We show that the qualitative features of this
interference effect arecaptured by the Smeagol COD described
above through an appropriate choice of parameters. Finally, we
note thatquantum interference in such multibranch structures leads
tothe appearance of large, internal countercurrents, which
exceedthe external current carried by the electrodes , these
calculations presented in this work were processing in Centre for
Nanoscale Dynamics and Mathematical Modeling in the Lancaster
universe uk .

In this research several main parameters/factors that effect on each of
structures of semi-conductor lasers have been investigated. This investigation
depends mainly on two procedures : (1) A classification for these parameters
including each of threshold current density, injecting threshold carrier density,
absolute threshold current density and their relations with each of number of
wells and cavity length.(2) A simulation process for the previous parameters
with variable values of number of wells and cavity length. Non ideal
contribution to the total output current like Auger recombination, Interface
recombination and Leakage recombination have also been estimated through the
simulation process. In other words, this research is devoted to specify the
optimum performance of multi-quantum well structure of semi-conductor laser.
The calculations were performed for a representative separate confinement
(MQW) laser structure in GaAs/AlGaAs system of: 7.5nm as a QW size, 250
nm as a thickness of the waveguide region and 8nm as a barrier size.