Al-Qadisiyah Journal of Veterinary Medicine Sciences

The effect of different types of red blood cells and temperature on haemagglutination of Paramyxo that cause Newcastle disease virus ( vaccinal strain) Lasota was studied. Results showed that the titer of vaccinal strain in haemagglutination test with red blood cells of chicken, turkey and (O) blood group of were 1:64 after 30 minutes and 1:16 in Cattle , sheep and rate through (45) minutes , In horse and buffalo 1:4 after 90min . The effect of temperature on haemagglutination of Newcastle dense virus with chicken red blood cells showed the titer 1:64 in room temperature at 28°C and 1:8 in refrigerator temperature at 4°C but give the titer of 1:16 incubator temperature at 37°C .

The study was aimed to investigate the prevalence of macroscopic and microscopic sarcocystosis in 428 goats ( male = 215, female = 213) slaughtered in Babylon province abattoirs.The macroscopic examination used to detected macroscopic cysts in carcasses and the microscopic examination (peptic digestion method, trichinoscopy, squeezing and histological examination) used to detect the microscopic cysts exists in esophagus, skeletal muscle, diaphragm and heart. The results were appeared macroscopic type infestation in goats with S. caprifelis reach 0.93 %. all the infection were found in esophagus with 0.93 % and didn’t appear in the other organs. we were found two types of macroscopic cysts which are fat & thin ones, it’s appear spindle or oval diffusing and embedding in muscular fibers of the infestation organs. The macroscopic cyst bradyzoites characterized by crescentic shape.The microscopic type S. capracanis in goats was 93.22 %, the percentage were variable depending on methods of examination, the higher percentage was found in peptic digestion method was 95.99 % then squeezing method was 91.22 % fallowing by trichinoscopy was 84.48 % and finally the histological examination method was 36.59 %. The microscopic cysts examinated by trichinoscopy appeared were in different shapes, it was oval, ellipsoidal, cylindrical and cone shaped and divided into locules interactive with each other contain the microscopic cyst bradyzoites which appeared as crescentic shape and have pointed anterior end and rounded posterior end in peptic digestion & squeezing methods.There were two type of bradyzoites, the first type was long thin arching and second type was the short fat and less arching, in addition to that the microscopic cysts appeared two different shapes by histological examination method which the first shape with thin wall and the other have thick and transversally striated wall.

The current research was aimed to study some chemical, quality changes in addition to the know the level of bacterial contamination for imported canned beef to Iraq in Diwaniyah city .The results of chemical analysis appeared that meat of Lebanese origin with Hana mark had a highest dry matter and fat percentage which were 44.35% and 19.475% respectively compared to other meat, while meat of Jordanian with Almaraaia mark had a highest protein and ash percentage were 14.925% and 5.025% respectively compared to other meat, and the highest percentage of carbohydrate was recorded for meat of Brazilian origin with Creat cow mark and was 5.425% .Also qualitative assessments, which included total volatile nitrogen were conducted and the highest value of its was for meat of Brazilian origin with xtrah mark was 13.100mg/100g of meat moreover Brazilian meat with xtrah mark recorded a highest average for Thiobarbituric acid 0.92 malonaldehid /kg meat the results revealed that amount of dye myoglobin was 168.500mg/100g of meat as the highest value in Almarraaia mark .On concerning bacteriological test the result were within limits allowed according to international standard where the number of total anaerobic bacteria ranged from 0-7 X 110 /gm of meat and the average of the numbers of Clostridium bacteria was 0-5 X 110 /gm of meat

Records of 600 Holstein cows were analyzed statistically to investigate the factors that effect the incidence of retained placenta. Blood samples were collected from 40 cows with retained placenta to estimate calcium, magnesium and phosphorus that associated with the incidence of retained placenta.Result of the present study showed that parity, season of calving, sex of calf, type of calving and level of milk production, were the most factors might cause or increase the incidence of retained placenta in Holstein cows. Blood levels of calcium, Magnesium and phosphorous significant decrease (7.82, 4.89, 4.66 mg/ml respectively) compared to normally expelled placenta (9.15, 6.39, 5.18 mg/ml respectively

A total of (80) samples of two types of cheese were collected (local cheese (40) and processed cheese (40)) during May-December of 2005 .The Microbial examination showed in crease their micro organisms content:-Means of total bacteria numbers in local and processed cheese were (7.2×108 )CFU)gr, 2.8×106 )CFU)gr) respectively .Means of coliform bacteria numbers were (6.6×106 )CFU)gr, 2.6×105 )CFU)gr) res.Mean of streptococcus (4.95×105 )CFU)gr, 2.9×104 )CFU)gr) res.Means of staphylococcus aureus numbers (8.6×105 )CFU)gr, 4.2×104 )CFU)gr) res.Means of psychotrophic bacteria numbers (8.6×106 )CFU)gr, 2.7×105 )CFU)gr) res.Means of thermphilic bacteria numbers (11.17×106 )CFU)gr, 3.0×106 )CFU)gr) res.Means of proteolytic bacteria numbers 5.7 × 106 )CFU)gr, 2.2×105 )CFU)gr) res.eans of liplytic bacteria numbers (5.1×107 )CFU)gr, 3.5×106 )CFU)gr) res.Means of spore forming b.

This study was conducted on (335) pregnant cow which infected with ephemeral fever disease.(238) cow was treated with antibiotic and antipyretic (187 cow multipara and 51 unipara) , and (97) cow was left with out treatment (69 cow multiparp and 28 cow unipara) when the signs of disease appear .Than the percentage of abortion and retained placenta estimated in two groups ( treated and non treated ) and also estimated the total abortion percentage according to pregnancy months. And also subjected to bacteriological study on secretions of aborted fetus to excluded the specific and nonspecific bacteria combined with abortion cases.The results of this study revealed the incidence of abortion in treated cow in ratio ( 10.5%) and (14.43%) in untreated cow; also the results showed that most abortion cases were occurred in the late three months of pregnancy especially in 8th month of pregnancy in ratio (41.02%) from the total cases of abortion.The study also showed the occurrence of retained placenta in treated and untreated aborted cows with ratio (72 , 85.71%) respectively.The results of bacterial study of aborted fetus secretions revealed that the rate of positive bacteriological isolation was (100%) from the total aborted cases ; the nonspecific bacteria formed the most of the bacterial isolation ,and recoded that the E.coli , Streptococcus spp. and Klebsiella spp. the highest rate of isolated microorganisms

This study was conducted to characterize the pathological changes result from infection with both F.gigantica and hydatid cyst in livers of cattle slaughtered in Al Hella abattoir a total of 327 livers were examined during period from 1st march to 30th November 2008 .55 of these examined cattle were infected with F.gigantica and 51 were infected with Hydatid cyst while 17 of these infected cases were infected with both F.gigantica and Hydatid cyst that’s mean the infection of liver with one of these two parasite not prevent from infection with another and these cases were isolated from females with age of more than 4 yearsthen took samples from livers which were with both infection for histopathological changes .Economical losses due to both infection was 26.300320 I.D.

The sensitivity of 20 gram-positive and gram- negative bacterial isolates obtained from patients suffered from respiratory diseases were studied. Gram- positive bacteria included: 4 isolates of both Staphylococcus aureus and Streptococcus pneumoniae and one isolate of Corynebacterium spp., while gram- negative bacteria consisted of Klebsiella pneumoniae (1 isolate), Klebsiella oxytoca (1 isolate), Escherichia coli (2 isolates), Pseudomonas aeruginosa (6 isolates) and Neisseria meningitidis (1 isolate). Camellia sinensis Linn leaf ethanolic extract antimicrobial activity was studied. We compared the mean zone of growth inhibition produced by green tea extract with that of the eight antibiotics used in this study. Green tea was effective against all tested gram- positive bacteria and some gram- negative bacteria, in which mean zone of growth inhibition of 14.66 mm in S. aureus, 14.5 mm in S. pneumoniae and 14 mm in Corynebacterium spp. was observed. As far as gram- negative bacteria, green tea extract produced the highest zone of growth inhibition of 15 mm against N. meningitidis and the lowest growth inhibition zone (8.5 mm) in case of E. coli and it was not effective in inhibition of P. aeruginosa, K. pneumoniae and K. oxytoca.The antimicrobial activity of green tea extract was similar to that of Neomycin when testing the sensitivity of N. meningitidis, while it was similar to oxytetracycline when testing S. aureus. The green tea extract gave low growth inhibition zones, but was higher than that reported by 5 antibiotics: (cefaclor, clindamycin, oxytetracycline, neomycin and cefotaxime) when studying S. Pneumoniae sensitivity. P. aeruginosa revealed wide antibiotic resistant, in which it resisted 7 antibiotics, followed by E. coli and S. aureus, as both of them resisted 6 antibiotics.