Abstract
Biosynthesis method of NPs is considered one of the most promising methods, aimed to biosynthesize ZnO NPs using edible truffles Terfezia claveryi and evaluate their antimicrobial activity by using the optimum concentration from among three different concentrations (8,10,13) μg/ml of ZnO NPs, as well as evaluation of their effectiveness as an antioxidant and 1anti-degradation of red blood cells, The study was conducted during from November 11, 2020 to October 22, 2021 in the Research Laboratory of the College of Education, University of Al-Qadisiyah and the Al-Ameen Scientific Laboratory for Scientific Research , ZnO NPs were initially characterized by UV spectroscopy, ( AFM), (SEM),to detect the size, shape and distribution of nanoparticles, as the shape of the ZnO NPs manufactured by Terfezia claveriy , ZnO NPs was tested towards the isolates of Gram-positive bacteria (Staphylococcus aureus, Enterococcus faecalis) and Gram-negative bacteria (Escherich coli, Klebsiella pneumonia and isolate of Candida albicans). ZnONPs showed the anti-bacterial activity, as the largest inhibition diameter of ZnO NPs 23 mm. 21mm in Escherichia coli and Klebsiella pneumonia bacteria at a concentration of 13 μg / ml respectively,. While there was no effect on the Candida yeast, and some antibiotics were tested to show their ability to inhibit pathogenic isolates, nanoparticles were used in synergy with three antibiotics that are considered the most inhibiting (Levofloxacin, Ciprofloxacin Amikacin), larger diameters were recorded when ZnO NPs was mixed with antibiotics. and its effectiveness as anti-cancer agents was evaluated using a series of nanoscale concentrations of ZnO NPs using the breast cancer cell line (MCF7) at 100 μg/ml gave a more toxic effect towards the (MCF7) . While there was no significant effect on the orientation of normal breast cells.
