JOURNAL OF KUFA FOR CHEMICAL SCIENCES

In this study we extract the the most important bioactive compound from Iraqi plant Tagets
pltula L. (ALcadifa, Algafary) by using column chromatography, and detected on thin layer
chromatography (TLC) plates in comparison with standard. Moreover, Fourier transform
infrared (FTIR), and Ultra violet- Visible (Uv-visible) spectrometer were used to confirm the
characterization of the extracted. The antimicrobial activity of extracts was tested against six
species of microorganisms: The Gram-positive bacteria; Staphylococcus aurous, and Gram
negative bacteria included; Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, and
Klebsiella pneumonia and Candida.

To achieve this work, Methyl- D- fructpyranoside (1) was synthesized first by treating D
Fructose with Methanol in acidic medium under thermodynamically controlled conditions to
make sure that the fructopyranoside are the predominant product. The hydroxyl group at C4
and C5 were converted into Isopropylidine (2) by treating (1) with Acetone in acidic medium,
and hydroxyl group on C1 and C3 were converted into mesylester (3) by treated (2) with two
equivalents of mesylchloride at 0Cº. The vital compound in this synthesis compound (4) was
obtained by treatment of (3) with two moles of sodiumazide in presence of TBAB. Reaction (4)
with unsaturated compound like maleicanhydride, p-benzoquinone, cinnamylalcohol,
acrylamide, benzalacetophenone and vinylacetate formed compounds (5), (6), (7), (8), (9) and
(10) respectively. The course of the reactions as well as the purity of products were monitored
by means of T.L.C. Identification of products were achieved by elemental analysis (C.H.N),
I.R. spectroscopy and 1HN.M.R. 17

A simple , sensitive and selective method has been developed for the determination of
Manganese (II). The method is based on the chelation of metal ion with 4-(4- Amino antipyrine
azo ) resorcinol (APAR)to form an intense color soluble product , that is stable and has a
maximum absorption at 486 nm , εmax of 1.62x104 L.mol-1.cm-1 . A linear correlation (0.1-1.2
µg/ml) was found between absorbance at λmax and concentration .The accuracy and
reproducibility of the determination method for various known amounts of Manganese (II)
were tested . The results obtained are both precise (%RSD was better than 0.462 % ) and
accurate (relative error was better than 0.2%) .The effect of diverse ions on the determination
of Manganese (II) to investigate the selectivity of the method were also studied .The stability
constant of the complex under optimized conditions and at room temperature was 7.8x107
L.mole-1 .The method was applied to the determination of Mn(II) ion in some standard
practical samples and the determination of the content of Mn (II) in fallen dust particles upon
AL- Kut governerate in Iraq .The fallen dust particles were collected form the buildings roof .

A new , simple , sensitive and rapid spectrophotometric method is proposed for the
determination of trace amount of cobalt (II) . The method is based on the formation of a 1:1
complex with 4-(4′- pyrazolon azo) pyrogallol (APAP) as a new reagent is developed . The
complex has a maximum absorption at 463 nm and εmax of 1.8 X 104 L. mol-1. cm-1 . A linear
correlation (0.05 – 1.5 μg. ml-1) was found between absorbance at λmax and concentration . The
accuracy and reproducibility of the determination method for various known amounts of
cobalt (II) were tested . The results obtained are both precise (RSD was better than 1.45 % )
and accurate (relative error was better than 0.6 % ) . The effect of diverse ions on the
determination of cobalt (II) to investigate the selectivity of the method were also studied . The
stability constant of the product was 5.71 X 104 L. mol-1 . The proposed method was
successfully applied to the analysis of dust and synthetic mixtures without any preliminary
concentration or separation.

In this study, new derivatives of D-Mannose have been synthesized . These derivatives
are esters of D-Mannose at anomeric center .Treatment of free sugar containing
hydroxyl groups such as D-Mannose with acetone using sulfuric acid (Conc.)as
catalyst lead to the formation of two five membered acetal rings ; 2,3:5,6-O
diisopropyldine-D-mannofuranose.

In this study Schiff basses(1) have been prepared by the reaction of 2-aminopyrimidine
with benzaldehyde derivatives then Thiazolidene-4-none (2) , imidazolidine-4-ones (3) ,
and oxazepine-4,7-diones (4) have been prepared from Schiff bases (1) by reaction with 2
mercaptoacetic acid ,glycine and malic anhydride respectively .1-phenyl-3-pyrimidin-2-yl
urea(5) and thiourea(6) have been prepared from reaction of 2-aminopyrimidine with
naphthylisocyanate and phenylisothiocyanate respectively.1,3-oxazolidine -4-one (7) ,3
phenyl-2,3-dihydrothiazol-2-yl pyrimidine amines (8)
have been prepared from the
reaction of urea or thiourea derivatives (5,6) with ethylchloroacetate ,p-phenylphenacyl
bromide respectively. 2-chloro N-(pyrimidin-2-yl)acetamide (10) have been prepared from
reaction of 2-aminopyrimidine
with α-chloroacetyl chloride .thenoxazole-2,5-
diamines(11) Thiazole-2,5-diamine (12) have been prepared by the reaction of the reaction
of (10) with urea and thiourea respectively. All prepared compounds are elucidated by
some spectroscopic methods (FTIR, H NMR ,C13 NMR).The biological and enzymatic
activity of some prepared compounds are evaluated

Background: Hypertension is a major health problem in adults, and contributes
to cardiovascular disease. Malnutrition or deficiency in different nutrients found to be
a risk factor for pathogenesis of essential hypertension. No clear association between
calcium or magnesium with blood pressure level could be identified. In this work
serum total, ionized, albumin corrected calcium and total and ionized Mg in primary
hypertension patients were measured and compared with healthy control.
Patients and Methods: Seventy-three uncomplicated, untreated, hypertensive,
but otherwise have no other systemic diseases males, ranging in age from (26–55)
years old, were entered into the study. Their blood pressure values were more than
95/140 mm Hg (seated posture). Control group consists of thirty healthy males with
normal blood pressure and their age range is between (22-46) years old. Serum
calcium and serum albumin were measured colorimetrically using ready for use kits.
Results: There is a significant decrease (p<0.05) in S.Total Ca, S.Ionized Ca, and
albumin-corrected Ca in hypertensive group in comparing with control group. No
significant change noticed in serum total and ionized magnesium, and in different
ratios between the groups.
Conclusion: Hypertensive patients have significantly lower serum total calcium
and Mg than control group. Calcium supplements may be required as adjuvant
treatment in addition to decrease NaCl in the diet to reduce excretion of calcium in
urine. In this work a new ratio (S.albumin/ S.calcium) is introduced as a useful marker
for calcium state. Further studies are required for other biochemical parameters in
larger patients sample size

A new system of functionally graded materials is considered in this study. The
samples were obtained by sintering titanium with hydroxyapatite powders (HAP) at
900◦c. Hydroxyapatite type grains were prepared by wet chemical method by addition
of H3PO4 to a suspension of Ca(OH)2 with close control of the all process parameters
like pH, reaction temperature, ageing, digestion time and sintering temperature.
The sintering conditions lead to convenient values of samples density as
compared with bone density. The bioactivity was tested in simulated body fluid (SBF).
The size of HAP type phases developed at the sample surface after soaking for several
weeks in SBF is about 100 µm and tends to form a continuous network of a new
developed bioactive layer.