Basrah Journal of Veterinary Research

This study was aimed to compare sanitary conditions by detection of hygiene statue of the
chicken breast meat taken from license and unlicensed slaughterhouses. Different
microbiological indicators were measured to determine some traits of the local fresh chicken
breast meat during different storage periods under refrigeration and freezing temperature. All
tests were made in the post-graduate laboratories of Animal Sciences Department, College of
Agricultural sciences, University of Sulaimani. The microbial content of breast meat in this study
showed that the TPC was increased from 3.63×104 to 8.56×104 CFU/gm meat and from 4.13×103
to 9.33×103 CFU/gm meat for both unlicensed and license slaughterhouses respectively, that
stored at fridge temperature. In freezing storage, total bacterial count was fluctuated in all
samples of breast meats during 90 days of storage. The coliform bacteria count in breast meat for
unlicensed slaughterhouse were increased from 7.65×103 to 1.14×104 CFU/gm meat and were
significant differences (P≤0.05) in all storage periods at refrigeration temperature were found. At
freezing temperature, coliform have found only in 0 and 15 days of storage for both unlicensed
and licensed. For psychrotrophic bacteria, the count have increased after 6 days of refrigerated
storage for both unlicensed and licensed slaughterhouses samples and significant differences
were obtained in most storage periods, whereas the count have fluctuated after 90 days of
freezing storage. In generally, the results microbial indicators were within the standard limits of
permission, Coliform bacteria count revealed that the number was higher than the acceptable
count (more than 103 CFU/gm meat) in case of unlicensed samples.

The present study investigated the presence of bla-VIM1in clinical isolates of
Pseudomonas aeruginosa. During the period from November 2017 to February 2018,
a total of two hundred patients admitted to (Al-Zahraa Teaching Hospital, Al-Karama
Teaching Hospital and Al-Kut Hospital for Gynecology, Obstetrics and Pediatrics) in
Wasit province. One hundred and three of isolates were diagnosed as P. aeruginosa.
High prevalence of P. aeruginosa isolates were detected in burn swab samples 70
(35%) followed by sputum 12 (6%) and ear 11 (5.5%). All 103 P. aeruginosa isolates
were primarily screened for carbapenems - resistance, 36 (34.95%) were resistant to
carbapenems. Carbapenems resistant isolates were underwent antimicrobial
susceptibility to 14 antibiotics using Kirby-Bauer disk diffusion method. The
phenotypic and molecular methods of carbapenem resistance were investigated.
Carbapenems resistant isolates were identified by the Double disc synergy test, which
detects the probability of isolates able to produce Metallo-beta- lactamases (MBL).
Out of the isolates 32 (88.89%)were positive. Imipenem-EDTA combined disk
method showed 30 (83.33%) of isolates possessed ability to produce the Metallobeta-
lactamases. In addition , the Modified Hodge test (MHT) showed the ability of
isolates to produce Carbapenemases enzyme. 16 (44.44%) of the isolates were
positive to Modified Hodge test. Carbapenemase gene were detected by PCR
technique. The results demonstrated that out of 36 carbapenems resistant
Pseudomonas aeruginosa (CRPA) isolates 34 (94.44%) were positive to bla-VIM1
gene.

This project was designed to evaluate the effect of low level leaser therapy
(LLLT) on the induced chronic defect in the tibial bone of rabbits radiographically ,
and physically,eight adults local breed rabbits of both sexes were employed to
induced 2.5 mm in diameter circular defect in the tibial bone surgically under general
anesthesia and highly aseptic technique ,the induced hole was implanted with 2.5 mm
stainless steel screw ,that removed from the bone after one and half month post
operation , the experimental animals randomly divided into two equal groups four
animas of each, the control group was lifted for normal healing process of the bone
defect without any treatment ,while the treatment group was exposed daily with single
dosage of continuous diode laser for five minutes of 850 nm and 148.4 j/cm2 at 72
hours interval for 14 days post operation (p.o.) ,the radiographic results in the
treatment group showed increase in the bone density around the induced defect with
new bone formation fill the cavity at the end of 2nd week p.o. then increase in the
bone density and volume at the end of 4th week p.o. compare with the control group
that the density and the new bone formation less than treatment group ,the physical
analysis revealed increase in the bone density and hardiness with significant increase
in fractures tolerance in the treatment group compare with the control group .In
conclusion that the LLLT successfully used for repairing the critical ( chronic) defect
in tibial bones in rabbits .

The present study was conducted to evaluate the effect of Cumin on diabetes mellitus induced by alloxan in rats. Male Wister rats were used, and randomly divided into five groups (6-8 rats for each group): The first group: was administered distilled water and served as normal control, the second group: induced diabetes by single subcutaneous injection of alloxan 100 mg/kg.body weight and served as diabetic control, the 3,4,5 groups was administered 50,100,200 gm/kg of rat's forage respectively for four weeks, then diabetes are induced in this groups by same route as above. Cumin treatment in all doses lead to significant decrease glucose levels, and triglyceride and significant decrease in cholesterol level in dose 100, 200 gm/kg of forage and no change occur in total protein level. These results indicate that cumin have a role in delaying of diabetes through the effects of cumin in decrease some biochemical parameters.

The present study was carried out to determine and investigate the
antihyperglycemic action of the alcoholic extract and alkaloids extracted from Iraqi
Medicinal Plant Haloxylon salicornicum on blood glucose level of Alloxan –Induced
Diabetic Rabbits (150 mg/kg intraperitoneal) . In present study were prepared are
alcoholic extract and isolated alkaloids . Preliminary qualitative tests were
carried out for all prepared extracts and identification bio active chemicals alkaloid
compounds (Acetic acid (amino carbonyl) ; 4-fluoro histamine ; hydroxyl urea; nhexyl
methyl amine ; 1-methyl dodecyl amine ; Octadomine) by TLC and GC-MS
chromatography . Alloxan –induced diabetic rabbits was prepared after injecting the
experimental rabbit with three dose of alloxan (I.V).Alloxan –induced diabetic rabbit
, the results indicated that alcoholic extract and alkaloids extracted from Haloxylon
salicornicum showed significant decreasing in glucose conc. levels. Alkaloids
isolated from Haloxylon salicornicum showed very high activity to decrease blood
glucose levels in hyperglycemic rabbits ,where significant decreasing (P<0.05)
was found at second hrs , significant decreasing (P<0.01) at fourth hr. and high
significant decreasing (P<0.001) at sex and twenty forth hrs. Both alcoholic extract