Abstract
Streptococcus pluranimalium was first isolated in 1999. Recently, several case
reports have been published that have revealed that S. pluranimalium can infect
humans as well. The pathogenicity and pathophysiology of this pathogen is poorly
studied and its characteristics are not well known. In this study, S. pluranimalium
was first isolated and then identified from infants and children who suffered from
upper respiratory infections. 90 samples were collected from nasopharyngeal cavity.
Among them, 83 Streptococcus spp. isolates were identified. 3 out of which were
biochemically and molecularly identified as S. pluranimalium. 16S rRNA
sequencing based identification revealed that all isolates showed a very close
identity match (92%) with subjected S. pluranimalium when blasted with gene bank
database. Furthermore, the antibiotic susceptibility of S. pluranimalium towards ten
antibiotics was conducted using Kirby-Bauer disk diffusion test. The results
revealed that S. pluranimalium was 100% susceptible to penicillin, levofloxacin, and
ofloxacin. However, other antibiotics showed diverse effects on S. pluranimalium.
reports have been published that have revealed that S. pluranimalium can infect
humans as well. The pathogenicity and pathophysiology of this pathogen is poorly
studied and its characteristics are not well known. In this study, S. pluranimalium
was first isolated and then identified from infants and children who suffered from
upper respiratory infections. 90 samples were collected from nasopharyngeal cavity.
Among them, 83 Streptococcus spp. isolates were identified. 3 out of which were
biochemically and molecularly identified as S. pluranimalium. 16S rRNA
sequencing based identification revealed that all isolates showed a very close
identity match (92%) with subjected S. pluranimalium when blasted with gene bank
database. Furthermore, the antibiotic susceptibility of S. pluranimalium towards ten
antibiotics was conducted using Kirby-Bauer disk diffusion test. The results
revealed that S. pluranimalium was 100% susceptible to penicillin, levofloxacin, and
ofloxacin. However, other antibiotics showed diverse effects on S. pluranimalium.
Keywords
16S rRNA sequencing
antibiotic susceptibility.
Streptococcus pluranimalium