Basrah Journal of Veterinary Research

A component of Fish extract has been isolated by a combination of 65% ammonium
’ sulphat salt precipitation and gel filtration. The purified allergen appeared as two major
peaks. Peak 1 1 exhibited the highest lglj binding inhibition and showed a specific
. allergenic activity about two fold higher than that of the peak 1. Peak 1 and peak 11 cross
reacted in IgE binding inhibition. Completely inhibiting the binding of specific IgE to each
other. The allergencity of purified allergen was not lost during purification process.

Random 'l‘n5 mutagenesis produced 2l cysteine/methionine and 13 methionine
au.'otrophic mutants of‘ Sinorliiznbimn me/1'/or/' strain Rmd20l. Linkage of Tn5 to
auxotroph indicated that each mutant had a single 'l‘n5 insertion. All auxotroph
showed spontaneous reversion to prototrophy and they resembled the parental strain
in production ofcell surface molecule and utilization of sugars and dicarboxylic acid.

The study was conducted on two groups of individuals. Group A (treatment)
consisted of l0 healthy volunteers who were administrated garlic (Allium sativum) for
thirty days and then followed for another 30 days without garlic. Group B(control) ll
healthy subjects served as a control. Garlic ingestion significantly lowered (p<0.05) in
diastolic B. Pr. in male and total after 7 days and non-significantly (p>0.05) in systolic B.
Pr. on male, female and total. Significantly lowered (p<0.05 and p<0.025) in diastolic
B.Pr. in male and total after 14 days respectively and non—significantly (p>0.05) in systolic
B.Pr. at male, female and total. Significantly lowered (p<0.025 and p<0.01) in diastolic
A B.Pr. in male and total after 21 days. Respectively and non-significantly (p>0.05) in
systolic B.Pr. at male, female and total. Significantly lowered (p<0.0l) in diastolic B.Pr. in
total and non-significantly (p>0.05) in diastolic B.Pr. at male and female whereas
significantly lowered (p<0.01) in systolic B.Pr. on female and non-significantly (p>0.05) in
systolic B.Pr. at male and female after 28 days. Non-significantly (p>0.05) in diastolic and
systole B.Pr. on male, female and total after 30 days without garlic.

Throug a complete year a 1000 cows were subjected to asexual health examination
two hundred and ten cases of affection with infertility problems were discovered in local
Iraqi cows (Al-Jenubi) and their crosses with Fresian .It was found that 88 cows ( 41.9 %)
as anestruse ,they were 28 cows (l3.33%) local cows and 60 cows (28.57%) crosses cows
and .the percentage of anestrus did not affected by certain seasonal trend, and 49 cows
(23.33%) were affected by repeat breeder,l7 cows (8.09%) of them were local cows and
32 cows (l5.23%) were crosses cows and the higher percentage of repeat breeders was in
summer season. Uterine infection were 73 cows (34.76%) cases devided as 45 cows
(2l.42%)local cows and 28cows (l3.33%) crosses cows and they were not affected by
acertianseason with the exception of their highest incidence in winter. the results shows
. that the percentage of cross cows higher than the percentage of local cows
signilicantly(p< 0.0l).
There is not significant correlation between seasons and affection cows with
infertility .But the results shows that the local breed is more resistant to infertility problems
than cross breed.

This study was conducted to investigate the effect of chitosan sheets on
A inflammation. Accordingly chitosan sheet was isolated and prepared from the
exoskeleton of the native shrimps in Basrah Province. The inflammation was induced and
evaluated by polyvinyl sponge to determine the events which occur during the
inflammatory process during different intervals in the presence or absence of chitosan
‘ sheets .For this purpose 24 male rabbits were used and two linear skin incisions (2 cm)
were made on the dorsal aspect in both sides of each rabbit. The inflammatory process at
l,3,7&l5 day post wounding was evaluated macroscopically in terms of hyperemia,
suppuration, dryness, and adhesion of margins and microscopically in terms of infiltration
of inflammatory cells specially neutrophils and macrophages, fibrovascular granulation
tissue. _
. The ‘results of macroscopic evaluation revealed that '-It first post wounding day the
test wound was significantly (p<0.05) more hyperemic than control wounds followed by
a significant decrease in the severity of hyperemia in test wounds at ls‘, 3rd, 7"‘, and 15"‘
post wounding days (p<0.05). The test wounds was more dry with less suppuration to
_ words the end of the experiment than control wounds (p<0.05). The adhesive contact
between the margin of the wounds was significantly higher in test wounds than in control
wounds through out the period of experiment (p<0.05)

Injection of l mg / kg of uranyle acetate caused an obvious changes in the numbers of white blood
cells with progressing time ofexperiment, specially in the numbers of neutropiiiles and monocytes which
decreased significantly, while the numbers of eosinophiles has increased significantly after the first week
of injection and then decreased significantly in the 2nd and 3rd week of exposure as compared with
control group. Also, the injection caused significant decreasing in Hb and PCV values, while the
activities of hepatic enzymes GPT, and GOT were not affected in comparison with control group.
. So, it may be useful to use this variation of WBC percentage as a primary indication to persons who D
exposed to different doses of radioactive compound

The study was carried out on (30) kidneys of fifteen adult clinically healthy sheep.
- The gross study ofkidneys, latex casts and corrosive casts of renal arteries were performed
as well as corrosive casts of ureter.
The study showed that the normal sheep kidney had a bean—shaped appearance
covered by transparent capsule . the casts preparation mapped the division of renal
arteries , within the renal hilus which divided into dorsal and ventral artery , each of them
I were bifurcated into cranial and caudal artery , the cranial Artery divided into three inter
lobar arteries , while the caudal artery divided into four inter lobar artery, each one
bifurcated into arcuate arteries from which multiple inter-Lobular arteries were detached.

Hypoglycemic activity of Haloxylon salicornicum and Glibenclamide were studied in
normal and diabetic rabbits. The drugs were adminstered once daily at dose of lg / Kg at
H.salicorm'cum and 10 mg / Kg at Glibenclamide by gavage for one month. Serum glucose
level, creatinine level, total bilirubine and total protein were reduced significantly in 15-30
days. The serum Transaminase (AST and ALT activities) had effect on the treated groups.

A total of 531 serum samples were subjected to Rose Bengal test (RBT) , serum
agglutination test (SAT) and indirect flurocent antibody test (IFAT) and ELISA.
The incidence of brucellosis was found to be 55.76%, 47.64% ,55.76% , and 58.70%
,on basis ofRBT.SAT.lFATand ELISA respectively . The agreement between the RBT and
. SAT was 85.47% and between RBT and IFAT was 100% ingiving apositive result. lncase of
ELISA the positive results was higher and we recommended using it as the defenitive test for
detection of brucellosis

A total of99 healthy genitalia ofAwassi ewes were collected from AL-Shula . and
local abattoirs , Baghdad province , for biometrical measurement of uteri . viz. .weight
and length of uterus , no. of caruncles in uterus body and horns . the greater and leaser
curvature length of both left and right horns and The diameters of both horns . The
results revealed that the mean weight and length of uterus respectively 34.365 g and
l.354 cm were significantly differs due to different phases of estrus cycle. The mean total
of caruncles in uterus body and homs \ere 3.566 and 90.939 which were insignificantl}
differ due U. different phases ofestrus respectively The greater lengths ofthe right ,and
left horn were 17.270 & l7.769 cm while the leaser lengths were I i. 974 & l2.542 cm
respectively which were insignificantly differ due to different oiuses of estrus .
However the mean different of the right & left horns were 1 £62 & 1 .339 cm
respectively which were significantly differ due to different phases of: strus; met estrus ,
showed the highest values

The protein extract from powder milk were prepared by extraction, followed by
purification and fractionation using gel filtraction. One peak was obtained from powder
milk with molecular weight of 22KDa.
ELISA and skin test were performed on 195 patients tested with powder milk.The
rate of positive results to skin test and ELISA was 60.51%.
- ‘ ‘ There were significant differences P<0.05 among age groups regarding the number
of patients who had positive skin test and ELISA results ,the mean value of flare diameter
and OD-values, in addition, a significant differences P<0.05 occurred among males and
females examined with powder milk

An allergic extracts from cow's, goat's and buffalo's milk were prepared with extraction,
followed by purification and fractionation by gel filtration, one major peak was obtained from cow,
goat and buffalo milk with molecular weight of23KD, 26 KD, ISKD respectively.
Total and specific IgE ELISA testing was performed on I37 patients serum samples. The
. rate of specific IgE positive ELISA results was 58%in ease of patients tested with goat milk
allergen and 57% in case of patients tested with buffalo milk allergen. There were significant
differences I’<0.05 among age groups, males and females regarding the rate of milk allergic
patients who had positive specific IgE ELISA results. .
In rural region ,the rate of patients who had goat's and buffalo's milk allergy was higher
than that in urban region. ‘
There was a cross-reaction among cow's milk extract protein , goat's and buffalo's milk
protein extract and the IgE binding capacity of buffalo's milk protein extract was higher than that of
goat's milk protein extract since lower concentration ofthis protein extract was needed to inhibit tip
to 50% the binding of specific IgE to cow's milk allergosorbent.

The effect of three dietary regimes: - high fat ((1-If), low protein (Lp), and High. fat low protein
(HfLp) was studied on 3 groups of 21 days old Balb/c mice .A fourth group received balanced diet as
' control (C). histological pictures of large intestine (colon) was examined after 3,6,l2 weeks after
weaning time (day 0) ofexperiment .body weight was determined on weekly bases for the four groups.
Significant differences were detected amongmeans of body weights ofexperimental animals in
comparison with control group as following: - Hf>C>HfLp>LP.
— ' - Profound histological changes of colon were observed specially in the Hflp group in process of
time. Significant differences in numbers of goblet cells and thickness ofthe adventitial layer of colon
was detected, the numbers of goblet cells were reduced in LP group, while their numbers increased in
Hf group.

Greater attention has been paid to the traditional medical practices in general and
herbal medicine in particular even in the developed countries. This study aimed to
investigate Allium porrum seeds for its potential contraction or relaxation effect on small
intestine in rabbits by using organ bath in vitro. In order to establish a dose-response
curve for the plant, six male rabbits were used in the experiment.
Pharmacological studies of the plant were done by comparing between intestinal
contraction after addition of the plant alone as agonist, and intestinal contraction after the
addition ofboth antaigon”ist’iidrugs then the plant extract.
A Allium porrum, showed a statistically significant dose-depend increase of intestinal
contraction.Atropine, adrenaline, and chlorpheniramine reduce the effect of A. porrum on
intestinal contraction. Both cholinergic and adrenergic mechanisms may involve the
action of the plant with more potent muscarinic action

Conjunctiva ofthirty rats and ten mice were examined for the presence ofthe normal flora
Streptobacillus moniliformis which is the causative agent of rat-bite fever in man . 60 % of each
rats and mice were found to harbor. this bacteria suggesting a potential source for human infection
. biochemical characterization revealed occurence of biotypes which are valuable in tracing
epidemics . A test for pathogenicity was carried out on rabbite

This study was conducted in Basrah at period from March through July 2000
.The protein extract from Bovine dander was prepared with extraction followed by
purification and fractionation by gel filtration into tow peaks. Elisa inhibition with
serum samples from 77 patients who were sensitive to bovine dander, revealed
similar lgE,lgG binding patterns for each peak. Also, there is a cross —reaction .
between these two peaks.PeakII exhibited the highest IgE binding inhibition and
showed a specific allergenic activity about two fold higher than that of peakI.The
allergenecity ofpurified allergen was not lost during purification process.

Three groups of male mice, ten mice each, were treated by gossypol acetic acid
for (38) day by oral rote successively: group one treated with normal saline served as
control, group two treated with 20 mg/Kg. BW. and group three treated with 40 mg/Kg.
BW. The treated males were direct faced individually with adult males for aggressive
behavior tests, and virgin female diestrus for sexual behavior tests. The results were as
follow: the group two and three did not mice show any copulation activities during the 3-
hour of observation, group two as well as three mice had less initiative in attacking their
opponents and tended to avoid any longer contacts in combating. Afterward group two and
three mice got significant body weight changes compared with group one (control).
Therefore, the suggested conclusion of the study was gossypol tended to reduce the
aggressiveness and sexual activity, but moreover body weight of the male mice was
decreased.

The present study is an attempt to determine the Etiology (Bacterial & Parasitic) of
Buffaloes Pulmonary Lesions. For this purpose samples of(105) affected lungs were collected
’ "from abattoirs. of the cities in southern part of Iraq named (Basra, Amara, Nassirya and the
Qurna). * 1
Samples of the investigation were obtained through the examination of a total of (917) lung
apparently healthy buffaloes examined in six months period from 1st of October 1980 to end
ofMarch 1990.
Among the encountered Pulmonary Lesions, (68) = eases ((>4.7(>%) were associated with
bacterial isolation and from (6) cases (5.71%) no etiology identified.
The various bacterial isolates associate with different pulmonary lesions are mentioned and it's
significance is discussed. Pulmonary parasitic infection were encountered in (31) cases
« (29.5%) of all types of lung affection observed among which one case (3.2%) was caused by
V Dict. viviparous and one case of migration of liver flakes (Fasciola gigantic) twenty nine
(93.2%) due to larval ofthe tape worm (echinococcus granulose ).

The heads ol‘ ten adult goats ol‘ both sexes were used to study the hypothalamic blood
supply .lt was found that the hypothalamus is ‘supplied by branches of cranial cerebral ,
cranial communicating arteries, caudal communicating as well as from tiny branches of
ophthalmic artery. The blood supply ol‘ mammillary body branches from the caudal
cerebral, communicating and last branches ol‘ basilar artery. The hypothalamus is rather
small in comparison to the rest of brain. Its blood supply is extremely rich , feeding from
many branches ofcranial cerebral arteries that forms the circle ofwillis. This indicates that
the hypothalamus has very high activity.

This study was conducted to investigate the effect of aqueous extract of Mentha
longifolia supplemented to broilers on some physiological parameters represented by
hematological characteristics .
A total of 90 chick of broiler faobre strain from IPA center for agricultural researches
were used at one day old . The birds at 4th day old , were randomly assigned into three
groups .
Each group consisted of thirty birds . The control groub ( c ) was not treated , T1
group treated with aqueous extract 1 ml / day of concentration 0.25 (gm / ml water) and
T2 group was treated with aqueous extract 1 ml / day of 0.125 gm/ml concentration and
for eight weeks .
All groups were fed the same commercial basal ration of broiler and the same
environmental condition and vaccinations were allowed to each group .
The results of blood samples tests were as follows .
Treated birds expressed high values of blood hemoglobin concentration and packed
cell volume percentage and showed significant difference ( p < 0.05 ) compared with
control group .
The birds ofT1 group indicated high significant
increased( p < 0.05 ) in total leukocytic count compared V
with the T2 & control group .
Theres is , significant increased ( p< 0.05 ) in numbers V
of TWBCS in T2 group compared with a control group .
A total erythrocytic counts revealed that there were Significant differences ( p <
0.05 ) between groupsln numbers of Red blood cells by increasing the number of red
blood cells in treated group .
These results refered to the benefit of aqueous extract of Men!/m /ongifolia to broiler
chicken at concentrations of 0.25 and 0.125 gm /ml