Samarra Journal of Pure and Applied Science

A simple electrochemical method was developed to estimate famotidine (FMT) in pure solutions and pharmaceutical preparatives by a flow-injection analysis system (FlA). This method includes preparation of a coated graphite electrode using FMT with the Phosphotungstic acid precipitant (PTA), di-butyl phthalate (DBP) and poly vinyl chloride (PVC). The electrode characteristics were examined by studying the optimum conditions, the carrier current, the injected model size, flow rate, the reaction coil length, pH, the range of concentrations, the minimum Nernst response, the response time, the detection limits, and the study also included identifying the electrode selectivity of the toward other interfering ions. The electrode showed that the best Nernst slope for the calibration curve was at 33.82 mv/decade within linear range of (1.0 × 10-6- 1.0 × 10-1M), correlation coefficient (R2) 0.9999, response time 10 seconds, the estimating speed is 90 sample/hour, while the rate of dilution degree (D) is 2.6452. The obtained results confirmed that it is of high accuracy and precision (Err 0.119%), (Rec 100.11%), (RSD 0.1233%). The proposed method was successfully applied to assay of FMT in commercial tablet forms.

Hypertension is a silent killer that increases disease infection and death rates, It is one of the most common diseases that greatly affect the heart, blood vessels, brain and kidneys. It is associated with high fat profile and is considered one of the most common risk factors for hypertension. The study included (89) samples at the rate of 57) a sample with high blood pressure disease consisting of (27) female and (30) male and (32) control samples consisting of (13) female and (19) male age ranged between (85- 30) years, the results indicated a significant increase in the concentration of apolipoprotein B in patients with hypertension, as it reached 2017 ng/ml compared to the healthy group, which amounted to ng/ml1830. Likewise, there was a significant increase in the level of cholesterol and triglycerides in patients with hypertension compared to healthy subjects, a significant significant decrease in the level of HDL-C concentration in patients with high blood pressure compared with healthy people, and a significant significant increase in the level of LDL-C and VLDL in patients with high blood pressure compared with healthy people., While the results showed that the concentration of blood glucose did not record significant differences between the two groups.

This study included preparation of the new Ligand of azo dyes Derivative of 4-aminoantipyrin Procedure was by couple aldiazoniom salt of amino compound 4-aminoantipyrin with acetyl acetone, the ligand were obtained (L1) ,second step was ligand complexity process (L1) with metal ions and salts) Ni(ІІ) ,Ag(І (, Hg(ІІ)( with ratio 2:1 ligand Identify these ligands by C.H.N.S analysis ,Uv-Visible spectra and infrared spectra .Different techniques have also been used to identify these complexes. Identify these complexes by C.H.N analysis to some of this complexes, Uv-Visible spectra . The infrared spectra of them were also studied .They show obvious changes with the ligands spectrum . New bands have been appeared which are not existed in the ligands spectra this was due to the combination of the metal ions with the donating atoms in the ligands .On the other hand ,other bands showed changes in the shape; position and intensity which is an evidence that a process of coordination had happened between the studied metal ions and the ligands. The conductivity measurements have of complexes in our research that. The magnetic susceptibility of the metal ions was also measured showing that the complexes of the Ni (ІІ) ions are paramagnetic. From the above observations, the geometrical structure of all complexes was octahedral, while complexes of the Ag(I) and Hg (II) the geometrical structure of complexes was Tetrahedral. As in the figure below. Ligand was studying bacterial efficiency and coordination prepared using two types of bacteria (Sehigella dysenteria, Bacillus pumilus) where he demonstrated ability to kill bacteria.

Acetyle CoA Carboxylase-1was purified from serum of premenop- ausal women with breast cancer (after Mastectomy or treatment ) by Gel Filtration using Sephadex G-100 and by Ion Exchange using DEAE-Cellulose A-50, also the molecular weight was estimated by the Electrophoresis on Acrylamide in the absence of denaturing elements . The result showed that a single band was obtained at 210 KD by Gel Filtering while Ion Exchange showed one band at 210 KD. The optimum temperature of purified Acetyle CoA Carboxylase-1 was 40 ℃ , optimal pH at 7.5 and the optimum substrate concentration at 1.8mM. Michaelis-Menten constant (km) was 0.3mM and Vmax was 23mM.min-1.

This research was conducted to purpose preparing and characterization various types of hydrogels such as blending, composite and grafting. This was done by reacting monomers such as acrylamide (A.M), methyl acrylate (M.A) and polymers. Polyvinyl Pyrrolidine (PVP) and carboxymethyl cellulose (CMC) with pectin was prepared from citrus peels. Hydrogel polymers (graft, blend and composite) were characterized using different techniques such as Fourier Transformed Infrared spectroscopy (FT-IR), Differential Scanning Calorimetry (DSC) and Scanning Electron Microscopy (SEM). The results of the reactions were identical to the theoretical expectations. The study was conducted to find out some properties such as swelling in water and absorption at different temperatures 25 °C and 20 °C, and the results were shown as a (559.9%, 429.2%) and (515.2%, 363.2%) respectively.

In this study, some of the (1- Phenyl-2-pyrazoline) compounds were synthesized by multistep synthesis. The first step was involved the reaction of phthalic anhydride with 4-Amino acetophenone to producing 2-((4-acetyl phenyl)carbamoyl)benzoic acid (A1), which subsequently dehydrated to the corresponding 2-(4-acetyl phenyl)isoindoline-1,3-dione (A2) by reaction with acetic anhydride and anhydrous sodium acetate, A2 was introduced in a condensation reaction in basic medium with various substituents aromatic aldehydes in a third step to produce α, β-Unsaturated ketone compounds (A3-A12), These compounds were reacted with phenylhydrazine and by using glacial acetic acid as a solvent to produce 1-phenyl-2-pyrazoline compounds (A13-A22) . The prepared compounds were characterized using infrared spectroscopy (FT-IR) and 1H-NMR and 13C-NMR for some of them. The antibacterial activity was also evaluated for these compounds.

This study was carried out in according to completely randomized design(CRD) in one of the private nurseries of the city of Ramadi / Anbar Governorate during the agricultural season of 2019. the aim of study was to determine the effect of nano-organic fertilizer and proline acid in some of the vegetative growth characteristics of Moringa oleifera . The study included three concentrations of nano-organic fertilizer (0, 50, 100) mg. L -1 and three concentrations of proline acid (0, 100, 200) mg. L -1, with three replicates, and after applying the studied factors and the passage of five months from the germination date : data were collected for the studied characteristics , statistically analysed , and the means were compared using the least significant difference (L.S.D) at the probability level 0.05. The results showed that spraying with organic fertilizer gave significant differences in the mean of the studied traits, since the treatment T1 achieved a concentration of 50 mg.L -1 (Plant length, leaf area, stem diameter, and dry weight of shoots ) either treatment T2 at a concentration of 100 mg. L -1 gave the highest rate in the two characteristics leaves number and dry weight of roots . As for spraying with proline acid, the treatment achieved T3 at a concentration of 100 mg. L -1 the highest rate in the two traits of dry weight of the vegetative group and stem diameter and treatment T4 at a concentration of 200 mg. L -1 achieved the highest rate in the rest of the other characteristics, namely (plant height, number of leaves, leaf area and dry weight of the Root) .The results also showed that the interaction between the organic fertilizer and proline concentrations achieved significant differences in some of the studied traits , as T6 treatment included 100 mg . L-1 of nano-organic fertilizer and 100mg. L-1 of proline acid gave the highest rate in plant height , number of leaves , leaf area and dry weight of shoots and roots .

factor field experiment with two factors was implemented according to the randomized complete blocks design with three replicates during the autumn cultivation season of 2019 for the period from 15/8/2019 to 30/1/2020 in one of the private fields in Fallujah city study the effect of adding some biological fertilizers and sprinkling some nutrients on the growth and yield of broccoli .A . The first factor included terrestrial applications as follows (without application T0 and application a fertilizer recommendation T1 and application half a fertilizer recommendation + trichoderma fungus T2 and application humic acid + trichoderma fungusT3) . The second factor included foliar spray, and it includes (without spraying D0 , spraying with biozyme D1, amino acid spraying D2, and licorice Glycyrrhiza glabra extract sprayD3) . The results showed that the effect of adding some biological fertilizers and foliar spray with some nutrients to a significant increase in (plant height, leaf area, total chlorophyll in the leaves, dry weight of the vegetative group, early flowering, weight of the main flower disc, total yield of floral discs, nitrogen in the flower discs Phosphorous in floral discs, potassium in floral discs.

The present study was designed to explore the biochemical properties and microbial activity of two natural plant species (Achillea conferta، Echinops charadinii). Biochemical analysis revealed the presence of different types of secondary metabolites such as (turbines، phenols and flavonoids). Achillea conferta showed a higher qualitative and quantitative amount of these compounds compared to E. charadinii. The methanol extracts of the two plant species were evaluatedfor antibacterial activity against five medically important pathogens: Staphylococcus aureus، Bacillus pumilus Escherichia coli، Pseudomonas aeruginosa، and one fungus Candida Albicans. The results showed different activities against all the tested pathogens in the different concentrations. Staphylococcus aureus was the most susceptible pathogen to A. conferta 100mg/ml in comparison to Tetracycline. (14.6 – 12.1 Respectively). The concentration 100 mg/ml was higher than all the other concentration in both species of plant in A.conferta scored (9.4-14.6) mm diameter of inhibition ,in E.charadinii scored ( 8.4-12.8 ) mm in all bacterial species except for the fungal species C.albicans wich did not record any inhibition, In conclusion، A. conferta methanol extract could treat diverse human bacterial pathogens due to its high antimicrobial activity.

This study aimed to isolate the antibiotics-resistant bacteria causing burns and wounds infections in military operation patients, and to compare the inhibitory efficacy of probiotics with the most common effective antibiotics. For this purpose, collected a total of 52 male patients of ages from 18 to 55 years suffering of wounds and burns infection from Military Muthanna Hospital form the period September 2019 to January 2020. Distributed between 34 of the samples were collected from wounds and 18 from burns. 27 (51.92%) from the wounds and 14 (26.92%) from the burns gave positive results for bacterial growth. A total of 61 bacterial isolates, 40 from wounds and 21 from burns, were obtained after culturing on MacConkey agar and blood agar and incubating at 37ºC for 24h. The isolates were identified by cultural, microscopic and biochemical tests and found to belong to both Gram positive and Gram-negative bacteria .The susceptibility test was performed by subjected each of the wound and burn isolates to four most common antibiotics to each isolate bacterium. Results showed that majority of the isolates were highly sensitive to Ciprofloxacin and Gentamicin. 8 of the highly antibiotic-resistant isolates were selected for treatment by two bacterial probiotics; Lactobacillus casei and L. paracasei Cultivated independently, in MRS broth (de Man, Rogosa and Sharpe broth) medium. The fermentation products were then concentrated (50, 25, and12.5) ml and the inhibitory activity of the eight most antibiotic-resistant isolates used was tested. Experiment was repeated three times and analysed with SPSS software. ANOVA Table with Tukey's Multiple Comparison Test. Results showed that despite the three-fold concentrated filtrates of both probiotics exerted good inhibitory activity against the pathogenic isolates, but L. casei filtrate was highly effective than that of L. paracasei. Moreover, L. casei filtrate was even competitor to Ciprofloxacin and Gentamicin.

The current research included studying the concentrations of some heavy elements (cadmium, zinc and copper) in the muscles, gills and liver of four types of fish (Leuciscus vorax, Chondrostoma regium, Carasobarbus luteus and Planiliza abu) quarterly catches starting from August 2018 until March 2019, the results showed the accumulation of heavy elements in the tissues and organs of fish (Leuciscus vorax, Chondrostoma regium, Carasobarbus luteus and Planiliza abu) in the two study stations and followed the following descending sequence: Leuciscus vorax > Carasobarbus luteus > Chondrostoma regium > Planiliza abu, and the results showed a difference in the accumulation of heavy elements in the tissues and organs of four types of fish, as the following descending order was followed by the liver> gills> muscles T, the accumulation of elements in which the annual rate and followed in descending order as follows: Zn> Cu> Cd. So the first station less polluted with three elements

The purpose of this paper is to study some definitions in general topology and generalized them to Micro topological space. These definitions are (Micro i-open and N-Micro open) set. The relations among these definitions with (Microopen, Micro i-open, N-Microopen, N-Micro semiopen, N- Microα- open,) set are studded, some new result are proved finally we define and study a new space namely N-Micro topological spaces.